Radio tlc scanner

⁸⁹Zr-labeled liposomes (0.67 ± 0.064 MBq (n = 3), 1 mg, 50 μL) in saline were added to human serum (0.5 mL) and diluted with saline (pH 7, 0.45 mL) to produce 50% serum. The stability of ⁸⁹Zr-labled liposomes was evaluated in 50% serum as in previously-reported liposomal studies ^{13 , 14 (link), 16} . Dilution of serum for such studies is typically performed to approximate the components of various proteins in blood. The ⁸⁹Zr-liposome serum mixture was incubated at 37 °C for 48 hours at a pH of 7. The serum mixture (100 μL) was drawn at 48 hours and isolated via a size-exclusion column (Sephacryl-300 HR, height: 300 mm, ID: 10 mm) with PBS (pH 7) as a mobile phase. Radioactivity and absorbance (280 nm) were measured by a radio- (Bioscan INC, NW) and UV- detector connected to the HPLC (Thermoscientific, Dionex UltimMate 3000). Liposomal stability was also evaluated with instant thin layer chromatography (ITLC, Biodex, NY). Liposomal serum mixtures (1 μL) were spotted on the ITLC and eluted with 0.1 M ammonium citrate (pH 5.5). Radioactivity was detected by a radioTLC scanner (Bioscan, NW).