Repo gal4

Manufactured by BD

Sourced in Germany

Repo-Gal4 is a laboratory equipment product. It serves as a core function, without further interpretation or extrapolation on its intended use.

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Lab products found in correlation

Elav gal4, by BD (4 mentions) Pdf gal4, by BD (2 mentions) Uas cd8 gfp, by BD (2 mentions) Repo qf2, by BD (2 mentions) Ddc gal4, by BD (2 mentions) Nsyb gal4, by BD (2 mentions) Th gal4, by BD (2 mentions) R54h02 gal4, by BD (2 mentions) Uas dcr2, by BD (2 mentions) Gmr gal4, by BD (1 mentions) Trh gal4, by BD (1 mentions) Uas draper rnai, by BD (1 mentions) Uas redstinger, by BD (1 mentions) Uas mcd8 gfp, by BD (1 mentions) Frt19a, by BD (1 mentions) Standard corn meal media, by Bloomington Drosophila Stock Center (1 mentions) Insc gal4, by BD (1 mentions) Tub gal4, by BD (1 mentions) Uas mcd8 rfp, by BD (1 mentions) Uas myrrfp, by BD (1 mentions)

13 protocols using repo gal4

Drosophila melanogaster Strains for Circadian Research

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The following strains of Drosophila melanogaster were used: w¹¹¹⁸, CantonS, yw;tim-Gal4 (Emery et al., 1998 (link)), UAS-belleRNAi (VDRC #6299), GMR-Gal4 (BDSC #8440), ninaE-Gal4 (BDSC #1104), repo-Gal4 (BDSC #7415), pdf-Gal4 (BDSC #6900), Eaat1-Gal4 (BDSC #8849), Lama-Gal4 (BDSC #35543), per⁰ (Smith and Konopka, 1981 (link)), yw;P{EPgy2}bel^{EY 08943} (BDSC #19945); P{PZ}bel^cap-1ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹ (BDSC #11778), bel::GFP (Morin et al., 2001 (link)) (Kyoto Stock Centre #ZCL2696), UAS-CD8-GFP (BDSC #5137). Co-IP was performed with yw; tim-GAL4/+; UAS-HAcry/+ (Dissel et al., 2004 (link)). Flies were maintained on a standard cornmeal medium under LD 12:12 regime and at constant 23°C.

Cusumano P., Damulewicz M., Carbognin E., Caccin L., Puricella A., Specchia V., Bozzetti M.P., Costa R, & Mazzotta G.M. (2019). The RNA Helicase BELLE Is Involved in Circadian Rhythmicity and in Transposons Regulation in Drosophila melanogaster. Frontiers in Physiology, 10, 133.

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Drosophila Neuronal Cell Labeling and Imaging

Cited 4 times

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The following stocks were employed - y[1] w[*]; Mi{y[+mDint2]=MIC}Syt1[MI02197] (BDSC#35973) (Venken et al., 2011 (link)), y(1) w(*) Mi(y[+mDint2]=MIC) Sh(MI10885) (BDSC#56260), y(1) w(*);Mi(y[+mDint2]=MIC)Shal(MI10881) (BDSC#56089) (Venken et al., 2011 (link)), y(1) w(*); Mi(y[+mDint2]=MIC) Shab(MI00848) (BDSC#34115) (Venken et al., 2011 (link)), nSyb-GAL4(GMR57C10)(BDSC#39171) (Pfeiffer et al., 2008 (link)), repo-GAL4 (BDSC#7415) (Sepp et al., 2001 (link)), repo-QF2 (BDSC#66477) (Lin and Potter, 2016 (link)), Shaw-GAL4 (BDSC#60325) (Venken et al., 2011 (link); Li-Kroeger et al., 2018 (link)), Ddc-GAL4(BDSC#7009) (Feany and Bender, 2000 (link)), TH-GAL4(BDSC#8848) (Friggi-Grelin et al., 2003b (link)), Trh-GAL4(BDSC#38389) (Alekseyenko et al., 2010 (link)), UAS_H2A-GFP (Sherer et al., 2020 (link)), QUAS_H2B-mCherry (Sherer et al., 2020 (link)), brp-GAL4 (this manuscript), UAS_H2A::GFP-T2A-mKok::Caax (this manuscript). All lines were raised on standard media at 25°C, 50% RH.

Jiao W., Spreemann G., Ruchti E., Banerjee S., Vernon S., Shi Y., Stowers R.S., Hess K, & McCabe B.D. (2022). Intact Drosophila central nervous system cellular quantitation reveals sexual dimorphism. eLife, 11, e74968.

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Drosophila Housing and Crossing Protocols

Cited 4 times

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Animals were housed at 25 °C with a 12-h light:dark cycle on standard cornmeal, agar, and molasses food media. For conditional studies with temperature-sensitive (ts) shibire^ts mutants^{8 (link),10 (link),40 (link)}, animals were raised at 18 °C with 12-h light:dark cycle until pharate pupal day 4 (D4), then transferred to 30 °C for 2 days before analyses. All lines were generated from the following stocks via either recombination or genetic crossing: (1) w¹¹¹⁸ ^{68 (link)}; (2) y,w;pin¹/cyo;repo-QF2/Tm6,tb (BDSC#66477); (3) QUAS-shibire^ts (BDSC#30012); (4) wg,sp/ cyo,draper^∆5/Tm6,Sb,Tb^{36 (link)}; (5) repo-Gal4/Tm3,Sb (BDSC#7415); (6) y,w; UAS-draper-RNAi/cyo^{36 (link)}; (7) w¹¹¹⁸;R86E01-Gal4 (BDSC#45914); (8) w¹¹¹⁸; R56F03-Gal4; (BDSC#39157); (9) w¹¹¹⁸; R54H02-Gal4 (BDSC#45784); (10) w¹¹¹⁸; dfmr1^50M/Tm6,Tb,GFP^{50 (link)}; (11) y,w; UAS-Draper-I (BDSC#67035); (12) w¹¹¹⁸; PDF-Gal4^{28 (link)}; (13) w¹¹¹⁸; UAS-mCD8::GFP^{45 (link)}; (14) w¹¹¹⁸; elav-Gal4^{29 (link)}; (15) y,w; UAS-dfmr1-RNAi (BDSC#35200); (16) y,w; UAS-InR^Del (BDSC#8248); (17) w¹¹¹⁸; shrub⁴/cyo^{67 (link)}; (18) w¹¹¹⁸; UAS-shrub^{67 (link)}; (19) w¹¹¹⁸; InR^E19(HR)⁶²; and (20) w¹¹¹⁸; InR^WildType(HR)⁶².

Vita D.J., Meier C.J, & Broadie K. (2021). Neuronal fragile X mental retardation protein activates glial insulin receptor mediated PDF-Tri neuron developmental clearance. Nature Communications, 12, 1160.

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Drosophila Melanogaster Genetic Toolkit

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Fly stocks are maintained in the standard cornmeal-based fly food in a 25°C incubator with humidity control. Larvae and adults are cultured in the light: dark (LD) condition with a 12-hour light: 12-hour dark light schedule. Unless otherwise noted, all larvae were collected between ZT1-ZT3 (ZT: zeitgeber time in a 12:12 hr light dark cycle; lights-on at ZT0, lights-off at ZT12). The following Drosophila melanogaster stocks were used for experiments: Obp44a-gRNA, UAS-Obp44a::GFP, UAS-CD2::mCherry, Obp44a::GFP CRISPR knock-in, and Obp44a CRISPR knock-out, are generated in this study. Obp44a-Gal4 (GMR90C03; BDSC 47122); UAS-CD8::GFP (BDSC 5137); UAS-RedStinger (BDSC 8547); Canton-S (wild-type for this study; BDSC 64349); UAS-Nsf2-RNAi (BDSC 27685); alrm-Gal4 (gifted by Dr. Marc Freeman); repo-Gal4 (BDSC 7415); elav-Gal4 (BDSC 458); repo-Cas9 (gifted by Dr. Chun Han); Pdf-Gal4 (BDSC 6899); UAS-GCaMP7f (BDSC 86320); tub-mito-roGFP2-Grx1 (BDSC 67669)

Yin J., Chen H.L., Grigsby-Brown A., He Y., Cotten M.L., Short J., Dermady A., Lei J., Gibbs M., Cheng E.S., Zhang D., Long C., Xu L., Zhong T., Abzalimov R., Haider M., Sun R., He Y., Zhou Q., Tjandra N, & Yuan Q. (2024). Glia-derived secretory fatty acid binding protein Obp44a regulates lipid storage and efflux in the developing Drosophila brain. bioRxiv.

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Transgenic Fly Strain Protocols

Cited 9 times

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Injections of transgenes for fly strains reported in this study were performed by Bestgene, Inc. Other fly stocks: repo-GAL4 (Sepp et al., 2001 (link)) (BDSC #7415), n-syb-GAL4 (Bushey et al., 2009 (link)), n-syb-QF2 (Riabinina et al., 2015 (link)), n-syb-LexA (Shearin et al., 2013 (link)), TrpA1-QF, and nompC-QF (Petersen and Stowers, 2011 (link)), QUAS-FLP (Potter et al., 2010 (link)), Brp-FRT-STOP-FRT-V5 (Chen et al., 2014 (link)), LexAop-GFP11 and UAS-CD4-GFP1-10 (Gordon and Scott, 2009 (link)), UAS-spGFP1-10::Nrx (Fan et al., 2013 (link)), UAS-syb::spGFP1-10 (Macpherson et al., 2015 (link)), vGlut-LexA (Baek et al., 2013 (link)), 24B-GAL4 (Brand and Perrimon, 1993 (link)).

Shearin H.K., Quinn C.D., Mackin R.D., Macdonald I.S, & Stowers R.S. (2018). t-GRASP, a targeted GRASP for assessing neuronal connectivity. Journal of neuroscience methods, 306, 94-102.

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Comprehensive Drosophila Genetics Toolkit

Cited 1 time

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FRT19A, fzr^A (#52384), FRT19A, fzr^B (#52385), and Dp(1;3)DC120 (#30265) were obtained from BDSC. FRT19A, fzr^G0418 (#111943) was obtained from Kyoto Stock Center. UAS-Fzr-HA (#F000893) was obtained from FlyORF. UAS-HA-Rca1 is a gift from Dr. Frank Sprenger (Grosskortenhaus and Sprenger, 2002 (link)) and FRT19A, fzr^8F3 (P{neoFRT}19A/FM7) was a gift from Dr. Christian Klambt. EGFP-Fzr^BAC was generated in this study.
RNAi lines used in this study: erm_RNAi (BDSC #26778), fzr_RNAi (GD#25553), cdc20_RNAi_1 (GD#40500), cdc20_RNAi_2 (GD#44834), ida_RNAi (BDSC#34522); brm_RNAi (GD#37720), β-gal_RNAi (BDSC#50680), and bcd_RNAi (GD#48966).
Neural stem cell drivers included insc-Gal4 (BDSC#8751; 1407-Gal4) or wor-Gal4 (BDSC#56553). Glial driver was repo-Gal4 (BDSC#7415). Type I NSC driver (ase-Gal4; UAS-mCD8-GFP, T. Lee). Type II NSC driver (w; UAS-Dicer2, wor-Gal4, ase-Gal80; UAS-mCD8-GFP) (Bowman et al., 2008 (link)). INP driver (erm-Gal4/CyO) (Pfeiffer et al., 2008 (link)). Other drivers used in this study are: nerfin-1-Gal4, UAS-mCD8-GFP (Louis Y. Cheng), pros-Gal4 (BDSC#80572), and elav-Gal4 (BDSC#458). UAS-Dcr2 (BDSC#24650) or/and UAS-CD8-GFP (BDSC#32186) was used together with various Gal4 drivers or RNAi stocks.
Experiments with mutants were performed at 25°C, and experiments for RNAi-mediated knockdown or overexpression were carried out at 29°C.

Ly P.T, & Wang H. (2020). Fzr/Cdh1 Promotes the Differentiation of Neural Stem Cell Lineages in Drosophila. Frontiers in Cell and Developmental Biology, 8, 60.

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Genetic Manipulation of Drosophila Neural Circuits

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All flies were maintained at 25°C on a 12-hour light/dark cycle and raised on standard corn meal media (Bloomington Stock Center). The following stocks were obtained from the Bloomington Drosophila Stock Center (Bloomington, IN): UAS-ATPα-RNAi (BDSC# 32913, RRID:BDSC_32913), 10XUAS-IVS-myr::tdTomato (referred to as UAS-tdTomato, BDSC#32222, RRID:BDSC_32222) and repo-GAL4 (BDSC# 7415, RRID:BDSC_7415). The ATPα::GFP reporter line was generated by the FlyTRAP project (Morin et al., 2001 (link)), and the UAS-ATPα line (RRID:FlyBase_FBst0500173) was obtained from FlyORF (Bischof et al., 2013 (link)). The following Dube3a and UBE3A lines were used in this study and described previously (Reiter et al., 2006 (link)): UAS-Dube3a, UAS-Dube3aC/A, and UAS-UBE3A. elav-GAL4 was provided by Dr. Hugo Bellen and UAS-GFP was provided by Dr. Cynthia Hughes. See Table S1 for a complete list of stocks.

Hope K.A., LeDoux M.S, & Reiter L.T. (2017). Glial overexpression of Dube3a causes seizures and synaptic impairments in Drosophila concomitant with down regulation of the Na+/K+ pump ATPα. Neurobiology of disease, 108, 238-248.

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Investigating Drosophila DNA Damage Repair

Cited 1 time

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The following fly stocks were used in this study: UAS-DDB1, UAS-DDB1^RNAi-Res, ddb1^HK-2-3, ddb1^W197 (generated in this study), ddb1^5-1, cul4^G1-3, cul4^JJ11, UAS-Flag-Cul4, UAS-Flag-Cul4^KR (CT Chien); mahj¹ [36 (link)]; UAS-Myc-Mahj, UAS-Myc-Mahj^R1120/1123E (this study). The following fly strains were obtained from BDSC: β-gal^RNAi (#50680), UAS-CD8-GFP (#32186), wts^RNAi (#34064), mahj^RNAi1 (#34912) and UAS-Yki^S168A (#28818); RNAi lines including ddb1^RNAi (#44974) and cul4^RNAi1 (#105668), cul4^RNAi2 (#44829) and mahj^RNAi2 (#110669) were obtained from the VDRC. Df(2R) XE2900 (#108418) is from the Kyoto Drosophila Genomics and Genetic Resource.
NSC drivers included insc-Gal4 (BDSC#8751; 1407-Gal4) or insc-Gal4, tub-Gal80^ts. Glial driver was repo-Gal4 (BDSC# 7145). Ubiquitous driver was tub-Gal4 (BDSC#5138). UAS-Dcr2 (BDSC#24650) was used together with various RNAi stocks. ddb1 and mahj RNAi knockdown efficiency was verified by immunostaining of anti-DDB1 and anti-Mahj antibodies in larval brains.
All experiments with mutants were carried out at 25 °C, and experiments for RNAi knockdown or overexpression were performed at 29 °C.

Ly P.T., Tan Y.S., Koe C.T., Zhang Y., Xie G., Endow S., Deng W.M., Yu F, & Wang H. (2019). CRL4Mahj E3 ubiquitin ligase promotes neural stem cell reactivation. PLoS Biology, 17(6), e3000276.

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Drosophila Neurological Genetics Toolkit

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Fly stocks were maintained using standard techniques, cultured at 25°C in 60% relative humidity. The following stocks were used in this study: Repo-Gal4 (BDSC #7415); R54H02-Gal4 (BDSC #45784); Nrv2-Gal4 (BDSC# 6799); UAS-Ube3A (BDSC# 90376); UAS-Wnk RNAi (BDSC #62150; #42521); UAS-Fray RNAi (BDSC #55878; #42569); UAS-HDAC4 RNAi (BDSC #28549; #34774); UAS-LexA RNAi (BDSC #67945); UAS-mCD8-RFP (BDSC# 32218); Sei^TS1, Eag¹Shaker¹²⁰ and NCKX^zydeco are gifts from Troy Littleton; UAS-RnSPAK^D219A, UAS-Wnk^WT, and UAS-Fray^T206E were gifts from Aylin Rodan.

Lones L, & DiAntonio A. (2023). SIK3 and Wnk converge on Fray to regulate glial K+ buffering and seizure susceptibility. PLOS Genetics, 19(1), e1010581.

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Fly Rearing and Gene Knockdown

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Flies were raised on standard yeast/cornmeal/agar media, at 25°C, 50% humidity and a 12-hour light/12-hour dark daily cycle, under uncrowded conditions. For the RNAi experiments, flies were raised at 29°C to reach the maximum efficacy of the apllied Gal4-UAS directed expression system. The following D. melanogaster stocks were used: Oregon R, Nrv2-GFP (BDSC stock no. 6828), repoGal4 (BDSC, stock no. 7415), UAS-CD2-HRP (BDSC, stock no. 9906), UAS-Dfabp RNAi (Transgenic RNAi Project—HMS01163), UAS-myr-RFP (BDSC, stock no. 7119), repoflp (gift from Christian Klämbt, Institut für Neurobiologie, Universitat Münster, Münster, Germany); UAS-Lsd2-EGFP (gift from Ronald P. Kühnlein, Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Germany), cortex glia specific Gal4 driver (NP2222, Kyoto Stock Center), Act > CD2 > GAL4 (gift from Gábor Juhász, Eötvös Loránd University, Budapest, Hungary), dfabp^EP3252 (Szeged Stock Center), Dfabp-GFP (115–074, Kyoto Stock Center). Oregon R flies were used as control for the histological experiment. For the RNAi experiments, control animals carried the same chromosome set except for the UAS-dfabp-RNAi transgene containing chromosome which was replaced with a wild type one (Oregon R).

Kis V., Barti B., Lippai M, & Sass M. (2015). Specialized Cortex Glial Cells Accumulate Lipid Droplets in Drosophila melanogaster. PLoS ONE, 10(7), e0131250.

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