Brains from eight WT and eight Celf6 mutant mice (see) were extracted, frozen in liquid nitrogen, and crushed into a fine powder, from which RNA was extracted using QIAGEN RNEasy columns on a Qiacube robot, following manufacturer’s protocol. RNA was DNase I treated, and RNA quantity and integrity were confirmed using Agilent Bioanalyzer. cDNA was prepared and chemically labeled with Kreatech ULS RNA labeling kit (Kreatech Diagnostics) and Cy5-labeled cDNAs were hybridized to Agilent Mouse v2 4×44K microarrays (G4846A-026655). Hybridization of the labeled cDNAs was done in Agilent 2x gene expression hybridization buffer, Agilent 10x blocking reagent and Kreatech Kreablock onto Agilent 4×44K V2 microarrays at 65°C for 20 min. Slides were scanned on an Agilent C-class Microarray scanner. Gridding and analysis of images was performed using Agilent Feature Extraction V11.5.1.1.
Agilent mouse v2 4 44k microarrays
Manufactured by Agilent Technologies
The Agilent Mouse v2 4×44K microarray is a genome-wide gene expression analysis tool designed for use with mouse samples. The microarray contains approximately 44,000 probes that target well-characterized mouse genes and transcripts. It provides a comprehensive coverage of the mouse transcriptome to enable researchers to study gene expression patterns and profiles in mouse models.
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2 protocols using agilent mouse v2 4 44k microarrays
1
Transcriptome Analysis of Celf6 Mutant Mice
2
Transcriptome Analysis of Celf6 Mutant Mice
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Brains from eight WT and eight Celf6 mutant mice (see) were extracted, frozen in liquid nitrogen, and crushed into a fine powder, from which RNA was extracted using QIAGEN RNEasy columns on a Qiacube robot, following manufacturer’s protocol. RNA was DNase I treated, and RNA quantity and integrity were confirmed using Agilent Bioanalyzer. cDNA was prepared and chemically labeled with Kreatech ULS RNA labeling kit (Kreatech Diagnostics) and Cy5-labeled cDNAs were hybridized to Agilent Mouse v2 4×44K microarrays (G4846A-026655). Hybridization of the labeled cDNAs was done in Agilent 2x gene expression hybridization buffer, Agilent 10x blocking reagent and Kreatech Kreablock onto Agilent 4×44K V2 microarrays at 65°C for 20 min. Slides were scanned on an Agilent C-class Microarray scanner. Gridding and analysis of images was performed using Agilent Feature Extraction V11.5.1.1.
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