The SEM images and EDS analysis were acquired using a Hitachi SU-8010 equipped with an EDX analyzer operated at an accelerating voltage of 5 kV. The TEM images were obtained on a JEM 2100 operating at 200 kV. The UV-vis diffuse reflectance spectroscopy (DRS) measurements were obtained on a UV-vis spectrometer (Shimadzu UV-2550) using BaSO4 as a reference standard. The specific surface area of the samples was measured by the Brunauer–Emmett–Teller (BET) method using nitrogen adsorption and desorption isotherms on a Micrometrics ASAP 2020 system. The UV-vis spectra were obtained on a Perkin Elmer Lamda 25 spectrophotometer. The NMR spectra were recorded on a Mercury Vx-300 MHz NMR spectrometer. XRD patterns were obtained on a Bruker D8-Advance. The luminescence spectra were measured using a Hitachi F-4500 spectrophotometer in MeOH at room temperature. A 500 W xenon lamp (CHFXQ 500 W, Global xenon lamp power) with a λ ≥ 420 nm optical filter, AM 1.5 optical filter and a heat cut-off filter provided visible light or simulated sunlight illumination.
Jem 2100
Manufactured by Shimadzu
Sourced in Japan
The JEM-2100 is a Transmission Electron Microscope (TEM) manufactured by Shimadzu. It is designed to provide high-resolution imaging of samples at the nanoscale level. The JEM-2100 utilizes an electron beam to magnify and focus the image of a specimen, allowing users to observe the internal structure and morphology of a wide range of materials.
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Lab products found in correlation
3 protocols using jem 2100
1
Characterization of Nanostructured Materials
2
Characterization of Chaxiong Oil Nanoemulsion
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The appearance and morphology of Chaxiong volatile oil nanoemulsion were studied by transmission electron microscopy (JEM-2100, Shimadzu, Japan) [27 (link)]. After a 5-fold dilution, the formulations were placed on a 400-mesh copper net coated with a carbon film and dried for 3–5 min at room temperature, the excess liquid was absorbed with filter paper, negatively stained with 2% phosphotungstic acid for 30 s, and placed for 2–3 min to dry naturally, then the morphology of nanoemulsion was observed under a transmission electron microscope. The morphology of nanoemulsions can be observed by scanning with a transmission electron microscope.
3
Characterization and Release Kinetics of Transferrin-Conjugated IR780 Nanoparticles
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The particle size and zeta potential were measured at room temperature by dynamic light scattering (DLS, 90Plus, Brookhaven Instrum. Corp). The morphologic and particle size examination of the Tf-IR780 NPs was further conducted by transmission electron microscopy (TEM, JEM-2100, Japan).
The absorptions of free IR780 and Tf-IR780 NPs dispersed in water were measured on an UV/vis spectrophotometer (UV-2450, Shimadzu, Japan) with a quartz cell with a 1 cm path length.
The release study of Tf-IR780 NPs at different pH values was performed. Predetermined amount of lyophilized nanoparticles were dissolved in 1 mL of phosphate buffer solution (PBS, 0.01 M, PH 7.4) and put into a dialysis bag (MWCO 3500). Then the bag was immerged into 15 mL release medium (PBS containing 0.1% v/v Tween 80) at different pH values (pH5.5, pH6.8 and pH7.4), and kept in an incubator at 37 °C. At predetermined time intervals, the release medium was withdrawn for UV–vis analysis. The amount of the IR780 was determined by UV–vis absorption spectra according to standard curve.
The absorptions of free IR780 and Tf-IR780 NPs dispersed in water were measured on an UV/vis spectrophotometer (UV-2450, Shimadzu, Japan) with a quartz cell with a 1 cm path length.
The release study of Tf-IR780 NPs at different pH values was performed. Predetermined amount of lyophilized nanoparticles were dissolved in 1 mL of phosphate buffer solution (PBS, 0.01 M, PH 7.4) and put into a dialysis bag (MWCO 3500). Then the bag was immerged into 15 mL release medium (PBS containing 0.1% v/v Tween 80) at different pH values (pH5.5, pH6.8 and pH7.4), and kept in an incubator at 37 °C. At predetermined time intervals, the release medium was withdrawn for UV–vis analysis. The amount of the IR780 was determined by UV–vis absorption spectra according to standard curve.
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