Bicuculline methobromide, CGP55845 hydrochloride, D-AP5, CNQX, QX314, and tetraethylammonium chloride (TEA) were purchased from Tocris Bioscience (Bristol, UK). 4-Aminopyridine (4-AP) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Bicuculline methobromide
Manufactured by Bio-Techne
Sourced in United Kingdom
Bicuculline methobromide is a chemical compound used in research applications. It functions as a competitive antagonist of the GABA-A receptor, which is a major inhibitory neurotransmitter receptor in the central nervous system.
Automatically generated - may contain errors
Lab products found in correlation
D ap5, by Bio-Techne (4 mentions)
Qx 314, by Bio-Techne (2 mentions)
Cgp 55845 hydrochloride, by Bio-Techne (2 mentions)
4 aminopyridine 4 ap, by Merck Group (1 mentions)
6 cyano 7 nitroquinoxaline 2 3 dione disodium salt hydrate cnqx, by Bio-Techne (1 mentions)
Complete freund s adjuvant cfa, by Merck Group (1 mentions)
Axio examiner d1 microscope, by Zeiss (1 mentions)
L apv, by Bio-Techne (1 mentions)
Bapta, by Merck Group (1 mentions)
D serine, by Bio-Techne (1 mentions)
Glutaraldehyde, by Bio-Rad (1 mentions)
8 cpt, by Bio-Techne (1 mentions)
Mk 801 maleate, by Bio-Techne (1 mentions)
Mouse anti myc, by Merck Group (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by GE Healthcare (1 mentions)
Rabbit anti ha, by Merck Group (1 mentions)
Mouse anti flag m2, by Merck Group (1 mentions)
Tetrodotoxin citrate ttx, by Bio-Techne (1 mentions)
Guinea pig anti vglut1, by Merck Group (1 mentions)
Rabbit anti myc, by Cell Signaling Technology (1 mentions)
7 protocols using bicuculline methobromide
1
Pharmacological Agents for Neurophysiology
2
Immune Modulator and Neuronal Inhibitor Protocol
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Complete Freund’s adjuvant (CFA) and Capsaisin (Cap) were purchased from Sigma-Aldrich. DL-2-Amino-5-phosphonovaleric acid lithium salt (APV), bicuculline methobromide (BIC), and 6-Cyano-7-nitro-quinoxaline-2, 3-dione disodium salt hydrate (CNQX) were purchased from Tocris.
3
Targeted Optogenetic Stimulation of ChR2-expressing Neurons
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To specifically activate selected subset of neurons expressing soma-tagged ChR2 in multiple neurons, targeted pattern illumination was performed using a digital micromirror device (Mosaic 2, Andor Technologies UK) mounted on Axio Examiner D1 microscope (Zeiss) connected to an X-LED1 light source.
Nuclear fluorescent tag (mRuby) was used to identify ChR2-expressing neurons and corresponding circular spots (12 µm diameter) were placed on individual cells. To illuminate individual spots with 470 nm blue light, Andor iQ 3.0 software (Andor Technologies, UK) and X-Cite XLED1 software (Lumen Dynamics) were used to control the micromirror array and XLED light source, respectively. IPSCs in response to stimulated spots were recorded at 0 mV in the presence of excitatory blockers (1 µM NBQX, 50 µM L-APV).
Bicuculline methobromide was purchased from Tocris Bioscience (Bristol, UK). All other chemicals and drugs were obtained from Sigma-Aldrich (Missouri, USA).
Nuclear fluorescent tag (mRuby) was used to identify ChR2-expressing neurons and corresponding circular spots (12 µm diameter) were placed on individual cells. To illuminate individual spots with 470 nm blue light, Andor iQ 3.0 software (Andor Technologies, UK) and X-Cite XLED1 software (Lumen Dynamics) were used to control the micromirror array and XLED light source, respectively. IPSCs in response to stimulated spots were recorded at 0 mV in the presence of excitatory blockers (1 µM NBQX, 50 µM L-APV).
Bicuculline methobromide was purchased from Tocris Bioscience (Bristol, UK). All other chemicals and drugs were obtained from Sigma-Aldrich (Missouri, USA).
4
Pharmacological Modulation of Neurons
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For the pharmacological modulation of neuronal activity, we used 1 μM TTX (Na+ Channel blocker; Cayman Chemical, USA), 20 μM bicuculline methobromide (GABAA receptor antagonist; Tocris Bioscience, Cambridge, UK), 100 μM 4-AP (K+ channel blocker; Tocris Bioscience, Cambridge, UK), 10 μM CNQX (AMPA/kainate receptor antagonist; Tocris Bioscience, Cambridge, UK), and 50 μM D-AP5 (N-methyl-d -aspartate receptor antagonist; Tocris Bioscience, Cambridge, UK). A total of >200 neurons from three different cultures were blindly analyzed for each condition.
5
Neurophysiological Experiment Reagents
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Pharmacological agents were purchased from:
Sigma Aldrich—BAPTA, bicuculline methobromide, TTX, paraformaldehyde, tannic acid, tergitol,
6
Immunofluorescence and Immunoblot Protocols
7
Neurotransmitter Receptor Antagonists
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Bicuculline methobromide, CGP 55845 hydrochloride, D-AP5, CNQX, and QX-314 were purchased from Tocris. Other reagents were purchased from Sigma or from Calbiochem.
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