Anti jam a clone j10.4

Manufactured by Santa Cruz Biotechnology

Anti-JAM-A (clone J10.4) is a monoclonal antibody that recognizes the Junctional Adhesion Molecule A (JAM-A) protein. JAM-A is a cell adhesion molecule involved in the regulation of tight junctions between cells.

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Lab products found in correlation

Dynabead, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti–JAM-A JAM-A

2 protocols using anti jam a clone j10.4

Cenicriviroc and HIV-Infected Monocytes

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Cenicriviroc was received from Allergan (Parsippany-Troy-Hills, NJ). Anti-JAM-A (clone J10.4) was from Santa Cruz Biotechnology (Santa Cruz, CA) and anti-ALCAM (clone 81) from Antigenix America (Huntington Station, NY). Purified IgG1 isotype control antibody was a gift from Chemocentryx (Mountain View, CA). THP-1, a monocytic leukemia cell line, and THP-1 cells infected with HIV 89.6 that express enhanced green fluorescent protein (GFP) under the control of the HIV promoter (THP89GFP) such that fluorescence and viral replication are coupled, were a gift from Olaf Kutsch (University of Alabama, Birmingham, AL) and were described previously (62 (link)).

Veenstra M., León-Rivera R., Li M., Gama L., Clements J.E, & Berman J.W. (2017). Mechanisms of CNS Viral Seeding by HIV+ CD14+ CD16+ Monocytes: Establishment and Reseeding of Viral Reservoirs Contributing to HIV-Associated Neurocognitive Disorders. mBio, 8(5), e01280-17.

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Preparation of Magnetic Beads for JAM-A Force Application

Cited 2 times

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Magnetic beads were prepared as previously described (Lessey-Morillon et al., 2014 (link)). Briefly, anti–JAM-A clone J10.4 (sc-53623; Santa Cruz Biotechnology) was conjugated to 4.5-μm tosyl-activated Dynabeads (ThermoFisher Scientific) in 0.1 M borate buffer, pH 9.5, according to the manufacturer’s protocol. After overnight incubation at 37°C with rotation, free sites were quenched by incubation with 0.1% fatty acid–free bovine serum albumin (BSA) for 1 h. For biochemical experiments, a continuous force calculated at ∼10 pN was applied to beads using a permanent ceramic magnetic (K&J Magnetics, Jamison, PA) as previously described (Guilluy et al., 2011b (link)).

Scott D.W., Tolbert C.E, & Burridge K. (2016). Tension on JAM-A activates RhoA via GEF-H1 and p115 RhoGEF. Molecular Biology of the Cell, 27(9), 1420-1430.

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