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Albumin fluorescein isothiocyanate conjugate albumin fitc

Manufactured by Merck Group
Sourced in United States

Albumin-fluorescein isothiocyanate conjugate (albumin-FITC) is a fluorescent labeling reagent consisting of the protein albumin covalently linked to the fluorescent dye fluorescein isothiocyanate (FITC). It is used as a tool in various biomedical research applications that involve the detection and visualization of proteins.

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3 protocols using albumin fluorescein isothiocyanate conjugate albumin fitc

1

Monocrotaline-Induced Cardiac Injury Model

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Monocrotaline (MCT), Mdivi-1, dimethyl sulfoxide (DMSO), Krebs-Henseleit (KH) buffer, sodium bicarbonate, calcium chloride dehydrate, heparin sodium salt and albumin-fluorescein isothiocyanate conjugate (albumin-FITC) were purchased from Sigma (St. Louis, MO, USA). The P110 peptide includes TAT to enhance membrane permeability. Both P110 and the peptide control sequence, TAT, were purchased from United Peptide (Herndon, VA, USA). Phosphate buffered saline (PBS) was purchased from Fisher Scientific (Fair Lawn, NJ, USA).
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2

Molecular Mechanisms of RNA Immune Activation

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Poly(I:C) HMW (synthetic double-stranded RNA with high molecular weight) was purchased from InvivoGen (San Diego, CA). IκBα inhibitor Bay11-7082 was purchased from EMD Chemicals (Gibbstown, NJ). Endosomal acidification inhibitor chloroquine (CQ), cycloheximide, and albumin-fluorescein isothiocyanate conjugate (albumin-FITC) were purchased from Sigma Chemical Co. (St. Louis, MO). Recombinant human interferon beta 1a (catalog #11415–1) was purchased from PBL Assay Science (Piscataway, NJ). Recombinant human interleukin-6 (catalog #rhil-6) was obtained from InvivoGen.
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3

Functionalized Silica Nanoparticles for VEGF Delivery

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Methanol,
tetraethyl orthosilicate (TEOS,
≥99.0%), triethyl phosphate (TEP, ≥99.8%), calcium nitrate
tetrahydrate (CNT, ≥98%), 3-aminopropyl triethoxysilane (APTES),
albumin–fluorescein isothiocyanate conjugate (FITC albumin), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide
hydrochloride (EDC), N-hydroxysulfosuccinimide sodium
salt (sulfo-NHS), albumin–fluorescein isothiocyanate conjugate
(albuminFITC), and 37% paraformaldehyde (PFA) were all
obtained from Sigma Aldrich. Aqueous ammonia (25%) was purchased form
Carl Roth. Absolute ethanol (EtOH) and phosphate-buffered saline (tablets,
PBS) were purchased from VWR. NHS–Fluorescein (5/6-carboxyfluorescein
succinimidyl ester) was purchased from Thermo Fisher. Human vascular
endothelial growth factor (VEGF165) was purchased from
Miltenyi.
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