Ab2712

Manufactured by Abcam

Ab2712 is a rabbit monoclonal antibody that recognizes the CYP1A2 protein. It is suitable for use in Western blotting applications. The antibody is affinity purified and provided in PBS buffer.

Automatically generated - may contain errors

Lab products found in correlation

Ab6584, by Abcam (1 mentions) Prolong gold mounting medium, by Thermo Fisher Scientific (1 mentions) Tissuefaxs imaging system, by TissueGnostics (1 mentions) Streptavidin dylight488, by Thermo Fisher Scientific (1 mentions) Clone c8 144b, by Agilent Technologies (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Ab21060, by Abcam (1 mentions) Ab3523, by Abcam (1 mentions) Ab5076, by Abcam (1 mentions) Rabbit igg, by Merck Group (1 mentions)

2 protocols using ab2712

Multicolor Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following de-paraffinization, antigen retrieval and blocking of peroxidase activity, whole slides were stained overnight at 4°C with primary antibody against TIA-1 (1:50, ab2712, Abcam). Sections were subsequently incubated with anti-mouse Envision+ reagent (K4000, DAKO) for 30 minutes and HRP visualized using cyanine 5 tyramide signal amplification (TSA) according to the manufacturer’s instructions (PerkinElmer). Next, whole slides were stained overnight with primary antibody against CD8 (1:25, clone C8/144B, DAKO) and a biotinylated antibody against fibronectin (1:50, ab6584, Abcam). Slides were incubated with GaM-AF555 (1:150 Life Technologies) and streptavidin-dylight488 (1:150, Thermo Scientific), counterstained with DAPI (Life Technologies) and mounted in prolong gold mounting medium (Life Technologies). Immunofluorescent slides were scanned using a TissueFaxs imaging system (TissueGnostics, Austria). Processed channels were merged using Adobe Photoshop CS5 (Adobe).

van Gool I.C., Eggink F.A., Freeman-Mills L., Stelloo E., Marchi E., de Bruyn M., Palles C., Nout R.A., de Kroon C.D., Osse E.M., Klenerman P., Creutzberg C.L., Tomlinson I.P., Smit V.T., Nijman H.W., Bosse T, & Church D.N. (2015). POLE proofreading mutations elicit an anti-tumor immune response in endometrial cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 21(14), 3347-3355.

+ Open protocol

+ Expand

Antibody Characterization for SYK Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibodies and sources used in this study were as follows: SYK (D3Z1E, Cell Signaling Technology or B01P, Abnova), phospho-SYK (Tyr525/526, 2711, Cell Signaling Technology), G3BP1 (611,126, BD Biosciences or 07–1801, EMD Millipore), pEIF2α (E90, Abcam), TIA-1(ab2712, Abcam), PABP1 (ab21060, Abcam), GFP (168AT1211, Abgent), GAPDH (6C5, Ambion), pTYR (4G10, EMD Millipore), IBA1 (ab5076, Abcam), GFAP (GF5, Abcam), TDP-43 (10,782–2-AP, Proteintech), NOS2 (ab3523, Abcam), Integrin β1 (4B7R, Santa Cruz Biotech), CD32 (ab197930, Abcam), and Rabbit IgG (Sigma-Aldrich).

Ghosh S, & Geahlen R.L. (2015). Stress Granules Modulate SYK to Cause Microglial Cell Dysfunction in Alzheimer's Disease. EBioMedicine, 2(11), 1785-1798.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!