Siphb2

Manufactured by RiboBio

Sourced in China

SiPHB2 is a laboratory instrument designed for small RNA sequencing. It utilizes a silica-based approach to efficiently extract and purify small RNA molecules from a variety of sample types. The core function of SiPHB2 is to enable accurate and reproducible small RNA analysis for research applications.

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Lab products found in correlation

Normal rabbit igg, by ABclonal (1 mentions) Rabbit anti ev a71 vp1, by GeneTex (1 mentions) Rt qpcr, by Thermo Fisher Scientific (1 mentions) Mouse anti β actin, by Proteintech (1 mentions) Rapamycin, by Selleck Chemicals (1 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions) Rabbit anti lc3b, by Cell Signaling Technology (1 mentions) Rabbit anti flag, by Proteintech (1 mentions) 3 methyladenine 3 ma, by Selleck Chemicals (1 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions)

2 protocols using siphb2

Investigating Autophagy Regulation via PHB2

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The following antibodies were used: rabbit anti-LC3B (Cell Signaling Technology, Boston, MA, USA, 3868), rabbit anti-PHB2 (Cell Signaling Technology, 14085), rabbit anti-SQSTM1/p62 (ABclonal Technology, Wuhan, China, A77580), normal rabbit IgG (ABclonal Technology, AC005), mouse anti-PHB2 (Proteintech, Wuhan, China, 66424-1-Ig), mouse anti-β-actin (Proteintech, 66009-1-Ig), rabbit anti-flag (Proteintech, 20543-1-AP), and rabbit anti-EV-A71 VP1 (Genetex, Irvine, CA, USA, GTX132339).
The following reagents were used: 3-methyladenine (3-MA; Selleck, Shanghai, China, S2767), rapamycin (Selleck, S1039), nontargeting siRNA (SiNC) and siRNA targeting PHB2 (SiPHB2) and ATP6AP2 (SiATP6AP2) (RiboBio, Guangzhou, China), Lipofectamine 3000 Reagent (Invitrogen, Carlsbad, CA, USA, L3000015), Lipofectamine RNAiMAX Reagent (Invitrogen, 13778-150), and primers for PCR and real-time quantitative PCR (RT-qPCR) (Invitrogen).

Su W., Huang S., Zhu H., Zhang B, & Wu X. (2020). Interaction between PHB2 and Enterovirus A71 VP1 Induces Autophagy and Affects EV-A71 Infection. Viruses, 12(4), 414.

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Transfection of lung cancer cell lines

Cited 1 time

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H1299, H460, A549, PC9, H1915, H2170, and human bronchial epithelial cells (HBE) were cultured as described previously [9 (link)]. Cells were transfected with si-PHB2 (RiboBio, Guangzhou, China), negative control, and PHB2-overexpressed plasmid (IbsBio, Shanghai, China) as described previously [9 (link)].

Zhang H., Yin C., Liu X., Bai X., Wang L., Xu H., Ju J, & Zhang L. (2020). Prohibitin 2/PHB2 in Parkin-Mediated Mitophagy: A Potential Therapeutic Target for Non-Small Cell Lung Carcinoma. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 26, e923227-1-e923227-10.

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