Ix70 inverted microscope

Manufactured by Hamamatsu Photonics

Sourced in Japan

The IX70 inverted microscope is a versatile optical instrument designed for a wide range of applications. It features a inverted configuration, which allows for easy sample access and manipulation. The IX70 provides high-quality imaging and observation capabilities, making it a reliable tool for various research and analysis tasks.

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Lab products found in correlation

Ultra low attachment surface 6 well plate, by Corning (1 mentions) Orca er camera, by Hamamatsu Photonics (1 mentions) Orca er ccd camera, by Hamamatsu Photonics (1 mentions) Image pro plus, by Media Cybernetics (1 mentions) Metamorph software, by Molecular Devices (1 mentions) Openlab software, by PerkinElmer (1 mentions) Orca er ccd digital camera, by Hamamatsu Photonics (1 mentions) Anti nestin, by BD (1 mentions) Orca flash4.0 v2, by Hamamatsu Photonics (1 mentions) Fluo 4 am, by Thermo Fisher Scientific (1 mentions) P35g 0 14 c, by MatTek (1 mentions) Dual optoled power supply, by Cairn Research (1 mentions) Flash 4, by Hamamatsu Photonics (1 mentions)

9 protocols using ix70 inverted microscope

Quantifying Anchorage-Independent Growth

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The ability of anchorage-independent growth was quantified as clonogenicity in 15% methylcellulose-containing full growth medium [28 (link)]. Cells were harvested by routine trypsinisation, dissolved in 15% methylcellulose-containing full growth medium and partitioned in triplicates onto ultra-low attachment surface 6-well plates (Corning); 20,000 cells per well were used. Colonies were counted after four weeks of culture, with supplementation with the normal growth medium every week. Photographic documentation was taken by the Olympus IX 70 inverted microscope equipped with the Hamamatsu Orca-ER camera. Experiments were performed in triplicates and repeated four times with similar results.

Kripnerová M., Parmar H.S., Šána J., Kopková A., Radová L., Sopper S., Biernacki K., Jedlička J., Kohoutová M., Kuncová J., Peychl J., Rudolf E., Červinka M., Houdek Z., Dvořák P., Houfková K., Pešta M., Tůma Z., Dolejšová M., Tichánek F., Babuška V., Leba M., Slabý O, & Hatina J. (2021). Complex Interplay of Genes Underlies Invasiveness in Fibrosarcoma Progression Model. Journal of Clinical Medicine, 10(11), 2297.

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Quantifying PGC Growth and Apoptosis

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SSEA1-positive PGCs with co-localised staining of phosphohistone-H3 and cleaved caspase-3 were counted from every other section of the entire length of the gonadal ridge of E10.5 embryos from each genotype. The percentage values are obtained by manually counting the total positive cells against the total cell counts labelled with DAPI. DAPI-positive cells negative for SSEA1 were counted as somatic cells. The PGC growth curves were generated by counting GFP-positive cells from 10 random fields of GR primary cultures plated on the NIH3T3 feeder layer at 0, 9, 18, 24, 32 and 48 h after plating. The percentage fold was calculated from the total cell count at 0 h. The images were captured using an Olympus IX70 inverted microscope (Hamamatsu C4742–95, Hamamatsu, Japan).

Lee J., Kim Y., Ataliotis P., Kim H.G., Kim D.W., Bennett D.C., Brown N.A., Layman L.C, & Kim S.H. (2023). Coordination of canonical and noncanonical Hedgehog signalling pathways mediated by WDR11 during primordial germ cell development. Scientific Reports, 13, 12309.

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Fluorescent Time-Lapse Imaging of Mitotic Proteins

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Fluorescent time-lapse videos were acquired with an Olympus IX-70 inverted microscope, a focused Xenon lamp, and an OrcaER CCD camera (Hamamatsu Photonics K.K., Massy, France), piloted by the Cell-R hardware and software system (Olympus, Rungis, France). Acquisition times per frame were 100 ms for GFP-Zw10 and 150 ms for RFP-Rod, and images were obtained as stacks of five planes at 1-μm intervals taken every 15 s with a ×60 NA 1.4 oil objective. For Fig. 4, images of a single plane were recorded every 5 s.

Défachelles L., Raich N., Terracol R., Baudin X., Williams B., Goldberg M, & Karess R.E. (2015). RZZ and Mad1 dynamics in Drosophila mitosis. Chromosome Research, 23(2), 333-342.

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Non-directional motility assay for cells

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Non‐directional motility was measured by time‐lapse live imaging using an Olympus IX70 inverted microscope (Hamamatsu C4742–95, Hamamatsu, Japan) at 37°C with 5% CO₂. Cells were serum‐starved for 24 h before treatments with DFM or purmorphamine for 18 h, and cell images captured every 15 min for 20 h were analysed using ImagePro Plus to track the movement (Media Cybernetics, Bethesda, MD). Twenty random cells were tracked in 3–5 independent repeat experiments.

Kim Y., Osborn D.P., Lee J., Araki M., Araki K., Mohun T., Känsäkoski J., Brandstack N., Kim H., Miralles F., Kim C., Brown N.A., Kim H., Martinez‐Barbera J.P., Ataliotis P., Raivio T., Layman L.C, & Kim S. (2017). WDR11‐mediated Hedgehog signalling defects underlie a new ciliopathy related to Kallmann syndrome. EMBO Reports, 19(2), 269-289.

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Visualizing Actin Filament Dynamics

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We generated time-lapse movies of actin filaments using prism-style total internal reflection fluorescence microscopy (TIRFM) on an Olympus IX-70 inverted microscope by collecting images every 10 s with a Hamamatsu C4742-95 CCD (Orca-ER) camera and MetaMorph software (Molecular Devices, Union City, CA). Images were processed with ImageJ software (http://rsbweb.nih.gov/ij/). For each sample, we measured the rates of barbed end elongation of 10-20 filaments, typically over a span of at least 300 s.
Glass flow chambers (Kuhn and Pollard, 2005 (link)) were incubated for 1 min each with 0.5% Tween-80 in high-salt TBS (HS-TBS) (50 mM Tris-HCl, pH 7.5, 600 mM KCl), 250 nM NEM-inactivated skeletal muscle myosin in Hs-TBS, and 10% BSA (w/v) in HS-TBS, with washes of HS-TBS after each incubation step. Polymerization was initiated by mixing 1.5 μM actin monomers (33% labeled with Oregon Green) with or without 5 μM profilin and varying concentrations of formin in standard microscopy buffer (10 mM imidazole, pH 7.0, 50 mM KCl, 1.0 mM EGTA, 1 mM MgCl₂, 1 mM EGTA, 0.3 mM ATP, 15 mM glucose, 50 mM DTT, 0.02 μM CaCl₂, 20 μg/mL catalase, 100 μg/mL glucose oxidase, and 0.5% methylcellulose [4,000 cP at 2% (w/v)]).

Baker J.L., Courtemanche N., Parton D.L., McCullagh M., Pollard T.D, & Voth G.A. (2014). Electrostatic Interactions Between the Bni1p Formin FH2 Domain and Actin Influence Actin Filament Nucleation. Structure (London, England : 1993), 23(1), 68-79.

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Immunocytochemistry of Neural Markers

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Cells were fixed with 4% paraformaldehyde (Electron Microscopy Sciences) for 15 min at room temperature. After rinsing with PBS, cells were incubated with blocking/permeabilizing solution, which consists of PBS with 10% donkey serum and 0.1% Triton X-100 for 1 hr at room temperature. Subsequently, cells were incubated with primary antibodies overnight at 4°C. Primary antibodies used in this study were anti-nestin (1:1000; BD Sciences, USA) and anti-beta-III tubulin (1:500; Covance). In order to visualize the primary antibodies, cells were incubated with fluorescent-labeled secondary antibodies (Alexa 488-Alexa 594-, or Alexa 647-labeled IgG; Life Technologies) for 1 hr at room temperature. Images of stained cells were captured using an Olympus IX-70 inverted microscope, equipped with an Orca-ER CCD digital camera (Hamamatsu) and OpenLab software (Improvision). ImageJ (Wayne Rasband National Institute of Health, USA) was used for image analysis. In addition, images were analyzed by CellProfiler (version 2.1.1; cellprofiler.org) for measuring fluorescent intensity. Three independent fields from each time point were used with matching exposure.

Moon J.I., Han M.J., Yu S.H., Lee E.H., Kim S.M., Han K., Park C.H, & Kim C.H. (2019). Enhanced delivery of protein fused to cell penetrating peptides to mammalian cells. BMB Reports, 52(5), 324-329.

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Calcium Imaging of Neuronal Cultures

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Calcium fluorescence recordings were carried out at 15 DIV using the cell-permeant calcium sensitive dye Fluo4-Acetoxymethyl ester (Fluo4-AM) (Invitrogen, Thermo Fisher), similar to [23 (link)]. Fluo4 1 mM dissolved in DMSO was added to the culture medium to a final concentration of 1 μM and incubated for 20 min at 37 °C. After incubation, cultures were placed in a 35-mm-diameter glass bottom chamber (P35G-0-14-C; MatTek Corporation) for recording. Recordings were carried out in pH-stable (7.4) external medium (EM). EM consisted of HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), 10 mM; NaCl, 128 mM; KCl, 4 mM; glucose, 10 mM; sucrose, 45 mM; CaCl₂, 2 mM; and MgCl₂, 1 mM. The recording chamber was mounted on an Olympus IX70 inverted microscope equipped with a Hamamatsu Orca Flash 4.0 V2 (Digital CMOS camera C11440-22CU) camera and Dual OptoLED power supply (Cairn Research Ltd) as a source of light. Fluorescence images were acquired at 20 frames per second (fps) at room temperature (RT) with a × 5 objective.

Fernández-García S., Orlandi J.G., García-Díaz Barriga G.A., Rodríguez M.J., Masana M., Soriano J, & Alberch J. (2020). Deficits in coordinated neuronal activity and network topology are striatal hallmarks in Huntington’s disease. BMC Biology, 18, 58.

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Monitoring Candida albicans Morphology

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To monitor C. albicans morphology, we used differential interference contrast (DIC) microscopy on an Olympus iX70 inverted microscope and a Hamamatsu FLASH4 complementary metal oxide semiconductor (CMOS) camera at ×40 magnification. For fluorescence microscopy, we used an X-cite series 120 light source with a 4′,6′-diamidino-2-phenylindole (DAPI) filter set. To monitor nuclei, cells were fixed and permeabilized with methanol before the addition of 1 μg/ml Hoechst stain and imaging. Representative images from two biological replicates are shown.

O’Meara T.R, & O’Meara M.J. (2021). DeORFanizing Candida albicans Genes using Coexpression. mSphere, 6(1), e01245-20.

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Magnetic Nanoparticle Uptake Imaging

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Suspension of Alexa Fluor 647-Fe@C NPs (MFMNPs) in distilled water was added to the culture medium until final concentration of 20 µg/ml. After 24 h of co-cultivation gold-plated permanent NdFeB magnet (5 × 5 × 5 mm³ cube, magnetic field 150 mT) was placed in Petri dish for 16 h. Cells were imaged by time-lapse confocal microscopy under 37 °C and 5% of CO₂ for the next 24 h on an Olympus IX70 inverted microscope equipped with CCD-camera Orca-RT+ (Hamamatsu, Japan) and controlled by Micromanager 1.4 software. Illumination conditions (neutral-density filters, lamp voltage, exposure time) were set to minimize photo toxicity.

Garanina A., Kireev I., Zhironkina O., Strelkova O., Shakhov A., Alieva I., Davydov V., Murugesan S., Khabashesku V., Majouga A., Agafonov V, & Uzbekov R. (2019). Long-term live cells observation of internalized fluorescent Fe@C nanoparticles in constant magnetic field. Journal of Nanobiotechnology, 17, 27.

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