Cell culture media and its supplements were purchased from Lonza, Inc. (Walkersville, MD, USA). The major enzymes such as α-amylase and proteinase K were attained from Sigma-Aldrich, St. Louis, MO, USA. The primary antibodies such as anti-phospho-NF-κB p65, anti-phospho-c-Jun N-terminal kinase (JNK), anti-phospho-extracellular signal-regulated kinase (ERK1/2), and anti-phospho p38, and secondary antibody (horseradish peroxidase-conjugated anti-rabbit antibody) were obtained from Abcam, Cambridge, UK. The antibodies for flow cytometry analysis such as anti-CD40-APC (1C10) and anti-CD11b (M1/70) were procured from ThermoFisher Scientific, Waltham, MA, USA.
Anti phospho nf κb p65
Manufactured by Abcam
Sourced in United Kingdom
Anti-phospho-NF-κB p65 is a laboratory reagent that can be used to detect the phosphorylated form of the NF-κB p65 subunit. NF-κB p65 is a key transcription factor involved in cellular responses to various stimuli, including inflammation, immune response, and cell growth and survival.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase conjugated anti rabbit antibody, by Abcam (2 mentions)
Proteinase k, by Merck Group (2 mentions)
α amylase, by Merck Group (2 mentions)
Cell culture media, by Lonza (2 mentions)
Anti phospho c jun n terminal kinase jnk, by Abcam (2 mentions)
Anti phospho extracellular signal regulated kinase erk1 2, by Abcam (2 mentions)
Anti phospho p38, by Abcam (2 mentions)
Anti cd11b m1 70, by Thermo Fisher Scientific (1 mentions)
Protease and phosphatase inhibitor mixture, by Merck Group (1 mentions)
Anti β actin, by Beyotime (1 mentions)
Anti phospho akt ser473, by Cell Signaling Technology (1 mentions)
Anti ikkα β, by Cell Signaling Technology (1 mentions)
Anti phospho erk1 2, by Cell Signaling Technology (1 mentions)
Anti phospho p38, by Cell Signaling Technology (1 mentions)
3 protocols using anti phospho nf κb p65
1
Molecular Signaling Pathways Profiling
2
Cell Culture Reagents and Antibodies
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Cell culture media and its supplements were purchased from Lonza Inc. (Walkersville, MD, USA). The major enzymes such as α-amylase and proteinase K were attained from Sigma-Aldrich, St. Louis, MO, USA. The primary antibodies such as anti-phospho-NF-κB p65, anti-phospho-c-Jun N-terminal kinase (JNK), anti-phospho-extracellular signal-regulated kinase (ERK1/2), and anti-phospho p38, and secondary antibody (horseradish peroxidase-conjugated anti-rabbit antibody) were obtained from Abcam, Cambridge, United Kingdom. The antibodies for ow cytometry analysis such as Anti-CD40-APC (1C10) and anti-CD11b (M1/70) were procured from ThermoFisher Scienti c, Waltham, MA, USA.
3
Immunoblotting Analysis of Key Signaling Pathways
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BMMφs were lysed in RIPA lysis buffer with freshly added protease and phosphatase inhibitor mixtures (Sigma-Aldrich). Lysates from VVH-treated BMMφs were resolved by SDS-PAGE, transferred to a nitrocellulose membrane, and subjected to immunoblotting analysis as described previously. Antiphospho-p38, anti-phospho-Erk1/2, anti-IKKα/β, and anti-phospho-Akt Ser473 were obtained from Cell Signaling Technology. Anti-phospho-NF-κB p65 was procured from Abcam, while anti-β-actin was obtained from Beyotime.
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