The long bones and hips of moribund quaternary transplant leukemic mice were harvested, cleaned, crushed, and sequentially passed through 100 μM and 70 μM filters (Falcon). Following red blood cells lysis (Qiagen), leukemic bone marrow cells were stained with APC-conjugated anti-c-Kit (clone 2B8; eBioscience) and Hoechst 33258 (H21491, Invitrogen) in PBS with 2% FBS (Omega). LSC-enriched populations were isolated by sorting Hoechst−, dsRed+, c-Kithigh (top 10–20 percent) cells (FACS Aria II, Becton Dickinson). In the quaternary transplant MLL-AF9 model, the c-Kithigh fraction is sufficiently enriched for leukemia-initiating cells, as described previously (Hartwell et al., 2013 (link); Miller et al., 2013 (link)). Quaternary transplant leukemia cells were used for experiments presented in Figures 1A–G , 2B–H , and 5C .
Apc conjugated anti c kit clone 2b8
Manufactured by Thermo Fisher Scientific
The APC-conjugated anti-c-Kit (clone 2B8) is a flow cytometry reagent used for the detection and identification of c-Kit-expressing cells. The c-Kit protein is a tyrosine kinase receptor that plays a crucial role in the development and regulation of various cell types. The APC (Allophycocyanin) fluorochrome is conjugated to the anti-c-Kit antibody, providing a bright signal for flow cytometric analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using apc conjugated anti c kit clone 2b8
1
Isolation of Leukemia-Initiating Cells
2
Enrichment and Generation of MLL-AF9 Leukemia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal experiments were conducted with an Institutional Animal Care and Use Committee–approved protocol. Mouse MLL-AF9 leukemias in a dsRed transgenic background (6051; The Jackson Laboratory) were generated as previously described (Miller et al., 2013 (link)). To enrich for LSCs, femurs and tibias from leukemic mice were first harvested, crushed, and filtered, and red blood cells were lysed using lysis buffer (BD). Then BM cells were stained with APC-conjugated anti–c-Kit (clone 2B8; eBioscience) and dsRed+c-Kithigh cells (20% high) were sorted (FACSAria II; BD). MLL-AF9–driven Tp53−/− leukemias were generated from c-Kit–enriched BM cells from Tp53−/− mice (002101; The Jackson Laboratory).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!