Rxi xlb gc

Acetonitrile, ethyl acetate, n-hexane (all GC grade), and sodium chloride (analytical grade) were supplied by Merck KGaA, (Darmstadt, Germany); AA (purity ≥ 99.9%), and the internal standard (IS) AA⁻¹³C₃, hydrobromic acid (48% w/w aq.), sodium thiosulfate pentahydrate, potassium bromide (both analytical grade) were purchased from Sigma Aldrich (St. Louis, MO, USA); bulk C18 (size 125 Å 55–105 µm) supplied by Waters (Milford, MA, USA); saturated bromine solution was supplied by Titolchimica (Pontecchio Polesine, RO, Italy). Agilent 7890A GC-MS system coupled to an Agilent 5975C mass selective detector (MSD) (Agilent Technologies, Santa Clara, CA, USA); the column was a Restek Rxi^®-XLB GC (proprietary phase; length × I.D.: 30 m × 0.25 mm; df: 0.25 µm) (Restek, Bellefonte, PA, USA).

All of the reagents that were used in this research were GC grade and were supplied by Merck KGaA, (Darmstadt, Germany), namely n-hexane, ethyl acetate, and acetonitrile (ACN). The salts and acids used in this study were supplied by Sigma Aldrich (St. Louis, MO, USA) and were analytical grade: potassium bromide, sodium chloride, hydrobromic acid (48% w/w), and sodium thiosulfate pentahydrate. The standards AA (purity ≥ 99.9%), and the internal standard (IS) AA−¹³C₃, were purchased from Supelco (Supelco Inc., Bellefonte, PA, USA); preparative C18 (125 Å 55–105 µm) was provided by Waters (Milford, MA, USA) and bromine water (3% w/v) was purchased from Titolchimica (Pontecchio Polesine, Italy). An Agilent (Model number 7890A) GC-MS system paired with an Agilent (Model number 5975C) selective mass detector (MSD) (Agilent Technologies, Santa Clara, CA, USA); the capillary column was a Restek Rxi^®-XLB GC (length × ID.: 30 m × 0.25 mm; df: 0.25 µm), supplied by Restek, Bellefonte, PA, USA).