Integrated laser engine

Images were acquired using an Andor Dragonfly spinning disk confocal microscope built on a Nikon Eclipse Ti fluorescence microscope. Images were collected using an Andor iXon Ultra 888 EMCCD camera through either a 20× air objective or Plan Apo Lambda 60× (NA1.4) oil objective. The system includes an Andor Integrated Laser Engine containing 100 mW 405 nm, 150 mW 488 nm, 150 mW 561 nm, and 140 mW 637 nm solid state lasers. The Nikon Perfect-Focus system was used to maintain focus in the z-plane during time-lapse imaging.

Images were acquired using an Andor Dragonfly spinning disk confocal microscope built on a Nikon Eclipse Ti fluorescence microscope. Images were collected using an Andor iXon Ultra 888 electron multiplying charge-coupled device camera through either an SF Apo TIRF 100× [numerical aperture (NA), 1.49] or Plan Apo Lambda 60× (NA, 1.4) oil objective. The system includes an Andor Integrated Laser Engine containing 100-mW 405-nm, 150-mW 488-nm, 150-mW 561-nm, and 140-mW 637-nm solid-state lasers. The Nikon Perfect-Focus system was used to maintain focus in the z plane during time-lapse imaging.

As described previously (15 ), images were acquired using an Andor Dragonfly spinning disk confocal platform together with a Nikon Eclipse Ti Fluorescence microscope. Images were taken using an Andor iXon 888 electron multiplying charge-coupled device camera through a Plan Apo Lambda 60× (numerical aperture = 1.4 oil objective), except where otherwise stated. The light source is an Andor Integrated Laser Engine containing 150 mW 488 nm, 150 mW 561 nm, and 140 mW 637 nm solid state lasers. Emission filters include 525/50, 600/50, and 700/75 nm. The Nikon perfect focus system was used to maintain focus during time-lapse experiments. Andor Fusion software (Oxford Instruments) was used to control the system and acquire images.