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Phospho merlin ser518

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Phospho-Merlin (Ser518) is a specific antibody that recognizes the phosphorylated form of the Merlin protein at serine 518. Merlin is a tumor suppressor protein that plays a role in regulating cell growth and motility. The phosphorylation of Merlin at serine 518 is an important regulatory event that can impact its function.

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Lab products found in correlation

Anti ctgf, by Santa Cruz Biotechnology (1 mentions) Phospho p38 thr180 tyr182, by Cell Signaling Technology (1 mentions) Vinculin, by Merck Group (1 mentions) Sorbitol, by Thermo Fisher Scientific (1 mentions) P yap ser127, by Cell Signaling Technology (1 mentions) Lats1, by Cell Signaling Technology (1 mentions) N cadherin, by BD (1 mentions) 2 deoxy d glucose 2 dg, by Merck Group (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Sp600125, by Bio-Techne (1 mentions) Yap taz, by Santa Cruz Biotechnology (1 mentions) Phospho mk2 thr334, by Cell Signaling Technology (1 mentions) Merlin nf2, by Cell Signaling Technology (1 mentions) Pi 4 5 p2, by Echelon Biosciences (1 mentions) Latrunculin b lat b, by Abcam (1 mentions) Sb203580 1202, by Bio-Techne (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions)

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Merlin (6 citations)

3 protocols using phospho merlin ser518

1

E-cadherin Immunostaining Protocol

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DECMA-1, a mAb recognizing the extracellular domain of E-cadherin (provided by Rolf Kemler, Max-Planck Institute for Immunobiology) was used to detect E-cadherin. A mouse mAb recognizing FLAG (DYKDDDDK) was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Mouse mAbs recognizing β-catenin and TAZ were purchased from BD Biosciences (Lexington, KY, USA); mAbs recognizing vinculin, actin, and pan-cadherin were obtained from Sigma-Aldrich Japan (Tokyo, Japan). Anti-Ki-67 was from Dako Japan (Tokyo, Japan). The rabbit mAbs, p44/42 MAPK (Erk1/2), phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204), Akt, phospho-Akt (Ser473), phospho-Merlin (Ser518), YAP, and the rabbit polyclonal antibody, phospho-Merlin (Ser518) were from Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-Merlin (NF2) and anti-CTGF (connective tissue growth factor) were from Santa Cruz Biotechnology (Dallas, TX, USA). Rabbit anti-cyclin D1 was obtained from MBL Japan (Nagoya, Japan). All secondary antibodies were obtained from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA).
, & Ozawa M. (2015). The N-cadherin cytoplasmic domain confers anchorage-independent growth and the loss of contact inhibition. Scientific Reports, 5, 15368.
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2

Immunoblotting and Microscopy Protocols

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The following antibodies were purchased from Cell Signaling and used at the indicated dilution for Western blot analysis: Merlin (NF2) (1:1000; 6995), phospho-Merlin Ser518 (1:1000; 9163), YAP (1:1000; 14074), pYAP-Ser127 (1:1000; 4911), LATS1 (1:1000; 3477), phospho-LATS HM (1:1000; 8654), phospho-MK2 Thr334 (1:1000; 3007), phospho-p38 Thr180/Tyr182 (1:1000; 4511), and HA HRP-conjugated (1:10,000; 2999). GAPDH (1:2000) and YAP/TAZ (1:500) were obtained from Santa Cruz Biotechnology (sc-25778 and sc-101199, respectively). Vinculin (1:5000) and Flag HRP conjugated (1:10,000) were from Sigma (V9131 and A8592, respectively. N-Cadherin (1:1000) was from BD Biosciences (610920).
The following antibodies were used for immunofluorescent microscopy experiments at the indicated dilutions: GFP (1:200) was from Abcam (ab6673), PI(4,5)P2 (1:200) was from Echelon Biosciences (Z-G045), Flag (1:500) and HA (1:500) were from Cell Signaling (14793 and 3724, respectively), and YAP (1:200) was from Santa Cruz Biotechnology (sc-101199).
Secondary antibodies Alexa fluor 488 and 555 and phalloidin were from Invitrogen and used in 1:1000 dilution.
Chemicals sorbitol was from Fisher Scientific (S459-500), latrunculin B (Lat B) was from Abcam (ab144291), 2-deoxy-D-glucose (2-DG) was from Sigma (D8375), and p38 inhibitor SB203580 (1202) and JNK inhibitor SP600125 (1496) were from Tocris.
Hong A.W., Meng Z., Plouffe S.W., Lin Z., Zhang M, & Guan K.L. (2020). Critical roles of phosphoinositides and NF2 in Hippo pathway regulation. Genes & Development, 34(7-8), 511-525.
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3

Western Blot Analysis of Merlin and Pak1/2

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Lysates from additional inflamed and non-inflamed animals (generated using the protocol described earlier) were run on 4–12% Novex tris-gly gels (Invitrogen). After transfer, membranes were probed with primary antibodies as follows: phospho-Merlin (Ser518) from Cell Signaling (13281) used at 1:1,000; phospho-Pak1/2 Ser144/Ser141 from Cell Signaling (2606) used at 1:1,000; Actin from Santa Cruz Biotechnology (sc-1616) used at 1:10,000; Gapdh used at 1:10,000 from Cell Signaling (D16H11). Secondary antibodies were anti-mouse and anti-rabbit IgG HRP-linked as appropriate (Cell Signaling 7076, 7074; used at 1:5,000).
Lyons J., Brubaker D.K., Ghazi P.C., Baldwin K.R., Edwards A., Boukhali M., Strasser S.D., Suarez-Lopez L., Lin Y.J., Yajnik V., Kissil J.L., Haas W., Lauffenburger D.A, & Haigis K.M. (2018). Integrated in vivo multi-omics analysis identifies p21-activated kinase signaling as a driver of colitis. Science signaling, 11(519), eaan3580.
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