Axioskop observer z1 microscope

Manufactured by Zeiss

The Axioskop Observer.Z1 is a high-performance microscope designed for advanced scientific and research applications. It features a modular design, allowing for customization to suit diverse research needs. The microscope offers exceptional image quality, with advanced optics and illumination systems to support detailed analysis and observation.

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Lab products found in correlation

Mouse anti myc, by Santa Cruz Biotechnology (1 mentions) Duolink in situ fluorescence kit, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Polysciences (1 mentions) Axio imager m2 microscope, by Zeiss (1 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions) E cadherin, by R&D Systems (1 mentions) Axio scan z1, by Zeiss (1 mentions) Gtx102425, by GeneTex (1 mentions) M7019, by Agilent Technologies (1 mentions) Af748, by R&D Systems (1 mentions) Prolong gold antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions)

3 protocols using axioskop observer z1 microscope

Proximity Ligation Assay in Transfected HeLa Cells

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Transfected HeLa cells, plated onto glass coverslips, were fixed with 4% PFA (PolySciences Europe) for 15 min at RT. Fixed cells were permeabilized with 0.1% Triton X-100 for 10 min, followed by blocking in PBS containing 10% horse serum for 1 h at RT. Cells were incubated with rabbit anti-p85 (1:1000, Millipore) and mouse anti-Myc (1:200, Santa Cruz Biotechnology) antibodies overnight at 4 °C. PLA was conducted using Duolink^® In Situ-Fluorescence kit (Sigma), following the instructions of the manufacturer. Images were captured using a Zeiss Axioskop Observer Z.1 microscope equipped with epifluorescence, with laser excitation filters at 548–572 nm and emission lines at 590–624 nm. Deconvoluted images were further analyzed with ImageJ software (NIH, Bethesda).

Vauthier V., Roujeau C., Chen P., Sarkis C., Migrenne S., Hosoi T., Ozawa K., Rouillé Y., Foretz M., Mallet J., Launay J.M., Magnan C., Jockers R, & Dam J. (2016). Endospanin1 affects oppositely body weight regulation and glucose homeostasis by differentially regulating central leptin signaling. Molecular Metabolism, 6(1), 159-172.

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Binucleate Superficial Cells in Bladder

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Binucleate superficial cells in convalescent bladders were examined by two methods. First, paraffin-embedded sections were stained and visualized on a Zeiss Axioskop Observer.Z1 microscope as described above. To determine the percentage of binucleate superficial cells, nuclei were enumerated in all superficial cells (TRP63⁻, keratin 5⁻, uroplakin IIIa⁺, keratin 20⁺ (weak/patchy staining observed in sensitized mice), with a basolateral band of E-cadherin). Adult naive bladders (n = 3) had 69–77 superficial cells, sensitized bladders (n = 6) had 135–202 superficial cells, and resolved bladders (n = 3) had 81–94 superficial cells, reflecting the smaller superficial cell size in sensitized and resolved mice. Next, binucleate cells were imaged in bladder whole mounts as described by Blango et al.^{49 (link)} with the following modifications: bladders were aseptically collected from adult naive, sensitized and resolved mice (n = 3 bladders per group), bisected, splayed, fixed for 20 min in 4% paraformaldehyde and washed 3 × 5 min in 1× PBS. Bladders were incubated for 10 min in 1:5,000 DAPI in 1× PBS with 0.002% saponin, rinsed 3 × 5 min in PBS and mounted in ProLong Gold antifade reagent (Thermo Fisher) and imaged on a Zeiss Axio Imager M2 microscope.

O’Brien V.P., Hannan T.J., Yu L., Livny J., Roberson E.D., Schwartz D.J., Souza S., Mendelsohn C.L., Colonna M., Lewis A.L, & Hultgren S.J. (2016). A mucosal imprint left by prior Escherichia coli bladder infection sensitizes to recurrent disease. Nature microbiology, 2, 16196.

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Immunofluorescence Analysis of Bladder Tissue

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Bladders were aseptically harvested and fixed overnight in methacarn (60% methanol, 30% chloroform, 10% glacial acetic acid), bisected to give two halves per bladder, paraffin-embedded and sectioned. For histopathological examination, slides were stained with hematoxylin and eosin and imaged with a Zeiss Axio Scan Z.1 brightfield slide scanner. Immunofluorescence experiments were conducted as previously described [15 (link)]. Primary antibodies used were uroplakin IIIa (mouse monoclonal, 10R-U103a, Fitzgerald), Trp63 (rabbit polyclonal, GTX102425, GeneTex), E-cadherin (goat polyclonal IgG, AF748, R&D Systems), cytokeratin 5 (chicken polyclonal, 905901, BioLegend) and cytokeratin 20 (mouse monoclonal, M7019, DAKO). Samples were mounted in ProLong Gold Antifade Mountant with DAPI (ThermoFisher Scientific) and fluorescence was visualized on a ZEISS Axioskop Observer.Z1 microscope.

O’Brien V.P., Dorsey D.A., Hannan T.J, & Hultgren S.J. (2018). Host restriction of Escherichia coli recurrent urinary tract infection occurs in a bacterial strain-specific manner. PLoS Pathogens, 14(12), e1007457.

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