1480 wizard 3

Manufactured by PerkinElmer

Sourced in United States, Finland

The 1480 Wizard 3 is a gamma counter designed for the detection and quantification of radioactive samples. It is a compact and automated instrument that provides accurate and reliable results for various applications, such as radioimmunoassay, receptor binding studies, and cell proliferation assays.

Automatically generated - may contain errors

Lab products found in correlation

Multiscreen filter plate, by Merck Group (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Prism software, by GraphPad (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Vivotag 800, by PerkinElmer (1 mentions) Dneasy blood tissue kit, by Qiagen (1 mentions) Low molecular weight chitosan, by Merck Group (1 mentions) 68ge 68ga generator, by Eckert & Ziegler (1 mentions) Icr mice, by Orient Bio (1 mentions) Lsc 5100, by Hitachi (1 mentions) 3h h2o, by PerkinElmer (1 mentions) Cell harvester, by Brandel Inc (1 mentions) Prism 8, by GraphPad (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) μbondapak c18 column, by Waters Corporation (1 mentions) Lc 10ad, by Shimadzu (1 mentions) C57bl 6, by Janvier Labs (1 mentions) 3000hs zetasizer equipment, by Malvern Panalytical (1 mentions)

30 protocols using 1480 wizard 3

Tissue Distribution of Radiolabeled Ser-PLL

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Ser-PLL was labeled with ¹¹¹In using DTPA according to a previously published method to determine the tissue distribution of Ser-PLL [19 (link),20 (link)]. The ¹¹¹In-labeled Ser-PLL was intravenously injected at 1 mg/kg dose into the tail vein of each ddY mouse. At appropriate time points after the injection, blood was collected from the abdominal vena cava under isoflurane anesthesia. The liver, kidneys, spleen, heart, and lungs were excised, rinsed with saline, blotted dry, and weighed. The blood was centrifuged at 2000× g for 5 min to obtain the plasma. The organ samples and 100 μL plasma were transferred to counter tubes and their radioactivity levels were measured with a gamma counter (1480 WizardTM 3′; PerkinElmer, Boston, MA, USA). The tissue distribution of PLL was determined as previously described, using ¹¹¹In-labeled PLL as a control.

Katsumi H., Kitada S., Yasuoka S., Takashima R., Imanishi T., Tanaka R., Matsuura S., Kimura H., Kawashima H., Morishita M, & Yamamoto A. (2022). L-Serine-Modified Poly-L-Lysine as a Biodegradable Kidney-Targeted Drug Carrier for the Efficient Radionuclide Therapy of Renal Cell Carcinoma. Pharmaceutics, 14(9), 1946.

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In Vivo Biodistribution of Radiolabeled and NIR-Labeled Ser-PAMAM-Cys Dendrimer

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Ser-PAMAM-Cys was radiolabeled with ¹¹¹In by a previous method [19 (link)]. ¹¹¹In-labeled Ser-PAMAM-Cys was intravenously administered at 1 mg kg⁻¹. At predetermined times post-injection, blood and major tissues were collected under isoflurane anesthesia. Radioactivity was measured with a gamma counter (1480WizardTM3”, PerkinElmer, Inc., Waltham, MA, USA) as previously described [14 (link)]. To visualize its distribution, Ser-PAMAM-Cys was labeled with the near-infrared fluorescence (NIR) VivoTag^® 800 (PerkinElmer, Inc., Waltham, MA, USA) according to the manufacturer’s instructions. NIR-labeled Ser-PAMAM-Cys was intravenously administered to ddY mice. Under isoflurane anesthesia, the mice were perfused with 10 mL saline through the left ventricle to flush out dendrimers in the blood or loosely bound to tissues. The liver, kidneys, spleen, heart, and lungs were excised and rinsed with saline. Ex vivo fluorescence images were acquired with an IVIS 60 min after intravenous injection as previously described [14 (link)].

Matsuura S., Katsumi H., Suzuki H., Hirai N., Takashima R., Morishita M., Sakane T, & Yamamoto A. (2018). l-Cysteine and l-Serine Modified Dendrimer with Multiple Reduced Thiols as a Kidney-Targeting Reactive Oxygen Species Scavenger to Prevent Renal Ischemia/Reperfusion Injury. Pharmaceutics, 10(4), 251.

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Cellular Uptake of Radiolabeled IUdR

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After incubation with ¹²³IUdR, the cells were washed twice in PBS. The cells were collected by centrifugation and the radioactivity of the cell pellet was measured in a gamma counter (1480 Wizard TM3, Perkin Elmer, Rodgau, Germany). To quantify the cellular uptake, cell numbers were determined with a cell counter (Casy Counter, Schärfe System, Reutlingen, Germany). The total accumulated decays per cell for a specific point in time were calculated using Excel (Microsoft, Redmond, USA), taking into account the half-life and time of measurement.

Unverricht-Yeboah M., Giesen U, & Kriehuber R. (2018). Comparative gene expression analysis after exposure to 123I-iododeoxyuridine, γ- and α-radiation—potential biomarkers for the discrimination of radiation qualities. Journal of Radiation Research, 59(4), 411-429.

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DNA Extraction from Radiolabeled Cells

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After incubation with ¹²³IUdR (50 kBq/ml), the cells were washed twice in PBS. Approximately 4 × 10⁵ cells were used for DNA purification using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). DNA was extracted according to the manufacturer’s protocol, except that the DNA elution step was done three times to increase the overall DNA yield. Additionally, the optional step using RNase (100 mg/ml, Sigma-Aldrich) to get RNA-free genomic DNA was performed. Radioactivity in the DNA was measured in a gamma counter (1480 Wizard^{TM 3}, Perkin Elmer).

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Radiolabeled RGD Peptide PET Imaging

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During the [⁶⁸Ga]NODAGA-RGD PET imaging, blood samples (2 ml) were obtained into heparinized tubes from the femoral artery 2, 5, 10, 20, 30, 40, 50 and 60 min after the injection of [⁶⁸Ga]NODAGA-RGD for the measurements of total radioactivity, radiometabolites (proportion of authentic tracer), and plasma-to-blood ratio. The radioactivities of whole blood and blood plasma were measured using a gamma counter (1480 Wizard 3″; PerkinElmer, Turku, Finland).

Grönman M., Tarkia M., Kiviniemi T., Halonen P., Kuivanen A., Savunen T., Tolvanen T., Teuho J., Käkelä M., Metsälä O., Pietilä M., Saukko P., Ylä-Herttuala S., Knuuti J., Roivainen A, & Saraste A. (2017). Imaging of αvβ3 integrin expression in experimental myocardial ischemia with [68Ga]NODAGA-RGD positron emission tomography. Journal of Translational Medicine, 15, 144.

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Quantitative Analysis of Tumor Uptake and Angiogenesis

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One hour and twenty minutes after injection of ¹⁸F-RGD-K5 and one hour after injection of ⁶⁸Ga-RGD, mice were sacrificed and dissected. The organs and tumours were removed, weighed and counted in a gamma-counter (1480 Wizard 3, Perkin Elmer). Uptake of tumours and tissues was expressed as mean ± SD percentage of injected activity per gram of tissue (%ID/g), corrected for ¹⁸F or ⁶⁸Ga decay.
All tumours were embedded in paraffin according to standard procedures and different stains were performed: haematoxylin-eosin (HE) for evaluating tissue appearance, tumour-proliferating antigen Ki67 for the tumour aggressiveness and CD31 for expressing the relative microvessel density (MVD) (%). The tumour slices were observed with an optical microscope in transmitted light (Nikon Eclipse, France). All quantitative analyses of the immunostaining were expressed as the percentage of positive area over the area of the entire section; they were measured by using Image J software.

Provost C., Prignon A., Rozenblum-Beddok L., Bruyer Q., Dumont S., Merabtene F., Nataf V., Bouteiller C, & Talbot J.N. (2018). Comparison and evaluation of two RGD peptides labelled with 68Ga or 18F for PET imaging of angiogenesis in animal models of human glioblastoma or lung carcinoma. Oncotarget, 9(27), 19307-19316.

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Radiolabeling and Characterization of Chitosan-TiO2 Nanocomposite

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Low-molecular weight chitosan (MW 50,000–190,000 Da, cP 20–300) and P25 (titanium(IV) oxide, 21 nm) were obtained (Sigma-Aldrich, Saint Lousis, MO, USA). For the labeling study, DOTA and NOTA chelator were obtained from Macrocyclic^TM. All optima grade acid solutions were purchased from Fisher Scientific (Waltham, MA, USA) and used without further purification. The ⁶⁸Ge-chloride source was obtained from Eckert & Ziegler (Berlin, Germany). ⁶⁸Ge/⁶⁸Ga generator (Eckert & Ziegler, Berlin, Germany) was used for a distribution coefficient of ⁶⁸Ga with 0.1 M HCl of an eluent. The activities of ⁶⁸Ge and ⁶⁸Ga were measured by a dose calibrator (Atomlab^TM, Biodex, New York, NY, USA). For measurement of ⁶⁸Ge breakthrough, a gamma counter (1480 WIZARD-3, Perkin Elmer, Trenton, NJ, USA) was used, and an in vivo study was conducted using a bench-top PET/X-ray system (Genesis 4, Perkin Elmer, Trenton, NJ, USA).

Lee J.Y., Choi P.S., Yang S.D, & Park J.H. (2021). TiO2 Decorated Low-Molecular Chitosan a Microsized Adsorbent for a 68Ge/68Ga Generator System. Molecules, 26(11), 3185.

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Binding Affinity of 18F-Exendin-4 to GLP-1R

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Example 4

Methods: INS-1 cells (stably transfected cells containing human proinsulin gene) were trypsinized and resuspended in RPMI 1640 medium (Life Technologies, Grand Island, N.Y.). Incubation was conducted with 96-well MultiScreen filter plates (Millipore, Mass.). Each well had a reaction volume of 200 μL containing 2×10⁵cells, 200 nCi (7.4 kBq) of ¹⁸F-Exendin-4 and 0-500 nM of unlabeled Exendin-4 or EB-Exendin-4. The reaction was incubated for 45 min on a shaker at room temperature. After incubation, cells were washed three times with RPMI medium buffer. Cell bound membranes were dried and isolated. The radioactivity was measured using a gamma counter (1480 Wizard 3, Perkin-Elmer). Binding results were expressed as percent of total counts, IC₅₀values were calculated using Prism software (GraphPad Software Inc., La Jolla, Calif.).

FIG. 1 shows the results of the above cell binding assay of Exendin-4 and EB-Exendin-4 with INS-1 cells using ¹⁸F-Exendin-4 as the radioligand. The IC₅₀values were determined to be 0.21±0.08 nM and 0.18±0.06 nM, respectively. As shown in FIG. 1, compared with Exendin-4, EB-Exendin-4 had similarly high binding affinity for GLP-1R (0.21±0.08 vs. 0.18±0.06 nM), indicating that EB conjugation did not comprise the binding affinity of the Exendin-4 peptide.

US10709790B2. Chemical conjugates of Evans Blue derivatives and their use in the production of long-acting therapeutics (2020-07-14). THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES [US]. Inventors: Xiaoyuan Chen [US], Lixin Lang [US], Gang Niu [US].

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Tissue Radioactivity Measurement Protocol

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Tissues of tumors and selected organs were weighed and collected into pre-weighed gamma counter tubes. The radioactivity of the tissues were counted using a gamma counter (1480 Wizard 3; PerkinElmer Life and Analytical Sciences) and counts per minute were decay-corrected. Results are expressed as a percentage injected dose per gram of wet tissue (%ID/g). Total activities injected per animal were calculated by evaluating the difference between pre-injection syringe counts and remaining syringe counts after injection.

Kim M.H., Kim S.G, & Kim D.W. (2022). A novel dual-labeled small peptide as a multimodal imaging agent for targeting wild-type EGFR in tumors. PLoS ONE, 17(2), e0263474.

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Biodistribution of Radioiodinated Compounds

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For a biodistribution
study, 25 male ICR mice (6 weeks old) were purchased from Orientbio
Co., Ltd (Jeonbuk, Korea Republic). The ICR mice were divided into
five groups so that each group contained five animals. Each mouse
was injected with an aqueous solution of [¹²⁵I] 9 or [¹²⁵I] 10 (100 μL, 1 μCi) through the
tail vein. After 0.5, 3, 6, 24, and 36 h post injection, a group of
mice were sacrificed under isoflurane dose; the organs of interest
(thyroid, lungs, stomach, heart, liver, kidneys, spleen, large intestines,
and small intestine) and blood were collected. The collected blood
and organs were weighed and the accumulated radioactivity was determined
using a 1480 wizard 3, (PerkinElmer, USA) gamma counter. The final
biodistribution data were reported in terms of the percentage injected
dose per gram of organ or blood (% ID/g). All animal experimental
procedures were approved by the Institutional Animal Ethical Committee
and performed according to the guidelines prescribed by the committee.

Mushtaq S., Nam Y.R., Kang J.A., Choi D.S, & Park S.H. (2018). Efficient and Site-Specific 125I-Radioiodination of Bioactive Molecules Using Oxidative Condensation Reaction. ACS Omega, 3(6), 6903-6911.

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