The samples of the solutions were analyzed via HPLC using the Shimadzu Prominence model LC-20 AD, equipped with a PDA detector and a C-18 column Luna® (150 mm × 4.6 mm i.d., 5 μm particle size, and 100 Å pore size) (Albert-Hahn-Str. 6-10, Duisburg, Germany). The column temperature was 40 °C. Eluent acetonitrile:water at a ratio of 40:60 v/v was used. An isocratic regime at a flow rate of 1 mL·min−1 was applied. The injection volume was 20 μL. CBZ was detected (UV) at 284 nm with a retention time of 4.95 min.
Prominence model lc 20 ad
Manufactured by Shimadzu
Sourced in Germany
The Prominence model LC-20 AD is a high-performance liquid chromatography (HPLC) system manufactured by Shimadzu. It is designed to provide accurate and reliable separation and analysis of various chemical compounds. The core function of the Prominence LC-20 AD is to facilitate the chromatographic separation and detection of analytes in liquid samples.
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HPLC Analysis of Carbamazepine
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HPLC Analysis of Compounds
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The HPLC analyses were performed using a Shimadzu Prominence model LC-20AD equipped with 20 mL injection loop, and a photodiode array detector and CUR detected at 420 nm. Data acquisition and analysis were carried out using LC solution software (1.25 SP2, Shimadzu, Mumbai, India, 2010). A C18 reversed-phase column (250 mm × 4.6 mm, particle size 5 μm) preceded by a C18 guard column (33 mm × 4.6 mm) was used for analysis. The mobile phase consisted of Acetonitrile- 5% acetic Acid (75:25, v/v) was run through the column at flow rate of 1.0 mL/min.
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