Endothelial cell growth medium supplement pack

Manufactured by PromoCell

The Endothelial Cell Growth Medium Supplement Pack is a collection of specialized growth factors and supplements designed to support the culture of endothelial cells in vitro. This pack provides the necessary components for the optimal growth and maintenance of endothelial cells derived from various sources.

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Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) M199 medium, by Thermo Fisher Scientific (1 mentions) D glucose, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Growth supplement (16 citations) Endothelial growth medium (11 citations) Growth Medium Supplement Pack (4 citations)

2 protocols using endothelial cell growth medium supplement pack

Culturing Primary Fibroblasts and HUVECs

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Primary fibroblasts were a kind gift from Søren R. Paludan (Aarhus University) and were cultured in high-glucose Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum (Gibco), 2mM L-glutamine (Gibco) and 1% penicillin/streptomycin (Gibco).
Primary, single-donor early passage HUVECs were kindly provided by Dr. Joanna Kalucka (Department of Biomedicine, Aarhus University, Aarhus, Denmark) and were obtained from Lonza, cultured at 37 °C, 5% CO₂ humidified incubators, and regularly tested for mycoplasma. HUVECs were maintained in M199 medium (1 mg/mL D-glucose) (Thermo Fisher Scientific) supplemented with 20% fetal bovine serum (FBS) (Biochrom BmgH), 2 mM L-glutamine (Thermo Fisher Scientific), Endothelial Cell Growth Supplement (ECGS)/ Heparin (PromoCell), 100 IU/mL penicillin and 100 mg/mL streptomycin (Thermo Fisher Scientific). For performed experiments, HUVECs were seeded in endothelial cell basal medium (EGM2) (PromoCell) supplemented with endothelial cell growth medium supplement pack (PromoCell). In all experiments, HUVECs were always used as single-donor cultures and were used between passages 2 and 4.

Kurmasheva N., Said A., Wong B., Kinderman P., Han X., Rahimic A.H., Kress A., Carter-Timofte M.E., Holm E., van der Horst D., Kollmann C.F., Liu Z., Wang C., Hoang H.D., Kovalenko E., Chrysopoulou M., Twayana K.S., Ottosen R.N., Svenningsen E.B., Begnini F., Kiib A.E., Kromm F.E., Weiss H.J., Di Carlo D., Muscolini M., Higgins M., van der Heijden M., Bardoul A., Tong T., Ozsvar A., Hou W.H., Schack V.R., Holm C.K., Zheng Y., Ruzek M., Kalucka J., de la Vega L., Elgaher W.A., Korshoej A.R., Lin R., Hiscott J., Poulsen T.B., O’Neill L.A., Roy D.G., Rinschen M.M., van Montfoort N., Diallo J.S., Farin H.F., Alain T, & Olagnier D. (2024). Octyl itaconate enhances VSVΔ51 oncolytic virotherapy by multitarget inhibition of antiviral and inflammatory pathways. Nature Communications, 15, 4096.

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HUVEC and Senescent Cells Cultivation

Cited 1 time

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Human umbilical vein endothelial cells (HUVEC) and senescent cells were prepared as previously described [18 (link)]. Briefly, 1×10⁴ cells per cm² were seeded in petri dishes coated with 1% gelatin and cultivated in RPMI-1640 complete medium supplemented with 5% fetal calf serum (FCS), 1% penicillin/streptomycin, and Endothelial Cell Growth Medium SupplementPack (C-39210, Promocell) at 37 °C in a 95% air and 5% CO₂ atmosphere. Cells of passages 1-3 (P1 to P3) were defined as young cells. Young cells were further split in a ratio of 1-3 continuously over the course of several weeks until replicative senescence was observed as determined by SA-ꞵ-gal staining (P9 to P12). In this study, non-senescent endothelial cells are referenced as “young cells” [18 (link)]. The model of S6K1 driving endothelial cells senescence was performed as described by Rajapakse et al. [18 (link)]. Lentivirus was used to transduce cells for gene silencing or overexpression as described previously [18 (link)]. THP-1 cells were grown in RPMI-1640 containing 10% heat-inactivated fetal bovine serum (HIFBS) and 1% penicillin/streptomycin [20 (link)].

Niu F., Li Z., Ren Y., Li Z., Guan H., Li Y., Zhang Y., Li Y., Yang J., Qian L., Shi W., Fan X., Li J., Shi L., Yu Y, & Xiong Y. (2023). Aberrant hyper-expression of the RNA binding protein GIGYF2 in endothelial cells modulates vascular aging and function. Redox Biology, 65, 102824.

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