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Magnetic 33 plex assay

Manufactured by R&D Systems
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The Magnetic 33-plex assay is a multiplex analysis tool that allows for the simultaneous detection and quantification of up to 33 different analytes in a single sample. It utilizes magnetic beads coated with capture antibodies specific to the target analytes. The assay provides a convenient and efficient method for researchers to analyze multiple biomarkers in a time-saving and cost-effective manner.

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Lab products found in correlation

Ionomycin, by Merck Group (2 mentions) Endotoxin core antibodies igm, by Hycult Biotech (2 mentions) Limulus amebocyte lysate assay, by Hycult Biotech (2 mentions) Soluble cd14 scd14, by R&D Systems (2 mentions) Ifabp, by MyBioSource (2 mentions)

5 protocols using magnetic 33 plex assay

1

Measuring Immune Mediator Production in LPLs

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LPLs (5 × 105/well) were thawed and incubated for 16 h in the absence (unstimulated) or presence (stimulated) of 5 ng/ml PMA and 1 μg/ml Ionomycin (Sigma-Aldrich, St. Louis, MO, United States) at 37 °C in a humidified incubator (5% CO2). At the end of the incubation, cells were centrifuged for 5 min at 2000 rpm. Supernatants were collected to measure the production of immune mediators using a Magnetic 33-plex assay (R&D Systems, Minneapolis, MN, United States) and a MAGPIX instrument (Luminex, Austin, TX, United States). Absolute change of immune mediators was calculated by subtracting the basal level of production from the level post-stimulation in a pairwise fashion. Bubble plots were generated using ggplot2 R package with concentration (pg/ml).
Rhoades N.S., Hendrickson S.M., Prongay K., Haertel A., Gill L., Edwards R.A., Garzel L., Slifka M.K, & Messaoudi I. (2021). Growth faltering regardless of chronic diarrhea is associated with mucosal immune dysfunction and microbial dysbiosis in the gut lumen. Mucosal Immunology, 14(5), 1113-1126.
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2

Multiplex Immune Mediator Profiling

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Plasma levels of immune mediators were assessed using a human 45-plex assay (Thermo Fisher Scientific, Waltham, MA, United States). Immune mediators produced by PBMC in response to PMA/ionomycin stimulation were measured using Magnetic 33-plex assay (R&D Systems, Minneapolis, MN, United States). All samples were analyzed using a MAGPIX instrument (Luminex, Austin, TX, United States).
Rhoades N., Mendoza N., Jankeel A., Sureshchandra S., Alvarez A.D., Doratt B., Heidari O., Hagan R., Brown B., Scheibel S., Marbley T., Taylor J, & Messaoudi I. (2019). Altered Immunity and Microbial Dysbiosis in Aged Individuals With Long-Term Controlled HIV Infection. Frontiers in Immunology, 10, 463.
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3

Immune Mediator Profiling of Stimulated LPLs

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LPLs (5 × 105/well) were thawed and incubated for 16 h in the absence (unstimulated) or presence (stimulated) of 5 ng/ml PMA and 1 μg/ml Ionomycin (Sigma-Aldrich, St. Louis, MO, United States) at 37 °C in a humidified incubator (5% CO2). At the end of the incubation, cells were centrifuged for 5 min at 2000 rpm. Supernatants were collected to measure the production of immune mediators using a Magnetic 33-plex assay (R&D Systems, Minneapolis, MN, United States) and a MAGPIX instrument (Luminex, Austin, TX, United States). Absolute change of immune mediators was calculated by subtracting the basal level of production from the level post-stimulation in a pairwise fashion. Bubble plots were generated using ggplot2 R package with concentration (pg/ml).
Rhoades N.S., Hendrickson S.M., Prongay K., Haertel A., Gill L., Edwards R.A., Garzel L., Slifka M.K, & Messaoudi I. (2021). Growth faltering regardless of chronic diarrhea is associated with mucosal immune dysfunction and microbial dysbiosis in the gut lumen. Mucosal Immunology, 14(5), 1113-1126.
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4

Quantifying Endotoxin-Related Immune Markers

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Levels of Endotoxin-core antibodies IgM (Hycult Biotech, Uden, Netherlands), soluble CD14 (sCD14) (R&D Systems, Minneapolis, MN, United States), and internal fatty acid-binding protein (iFABP) (MyBioSource, San Diego, CA, United States) in plasma samples were measured using an enzyme-immunoassay technique (ELISA) following the manufacturer’s protocol. Plasma levels of free bacterial endotoxin were measured using a chromogenic Limulus amebocyte lysate assay (Hycult Biotech, Uden, Netherlands) following the manufacturer’s protocol. Plasma levels of circulating immune factors were measured using a Magnetic 33-plex assay (R&D Systems, Minneapolis, MN, United States) and a MAGPIX instrument (Luminex, Austin, TX, United States). Bubble plots were generated using ggplot2 R package with concentration (pg/ml).
Rhoades N.S., Hendrickson S.M., Prongay K., Haertel A., Gill L., Edwards R.A., Garzel L., Slifka M.K, & Messaoudi I. (2021). Growth faltering regardless of chronic diarrhea is associated with mucosal immune dysfunction and microbial dysbiosis in the gut lumen. Mucosal Immunology, 14(5), 1113-1126.
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5

Quantifying Endotoxin-Related Immune Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Levels of Endotoxin-core antibodies IgM (Hycult Biotech, Uden, Netherlands), soluble CD14 (sCD14) (R&D Systems, Minneapolis, MN, United States), and internal fatty acid-binding protein (iFABP) (MyBioSource, San Diego, CA, United States) in plasma samples were measured using an enzyme-immunoassay technique (ELISA) following the manufacturer’s protocol. Plasma levels of free bacterial endotoxin were measured using a chromogenic Limulus amebocyte lysate assay (Hycult Biotech, Uden, Netherlands) following the manufacturer’s protocol. Plasma levels of circulating immune factors were measured using a Magnetic 33-plex assay (R&D Systems, Minneapolis, MN, United States) and a MAGPIX instrument (Luminex, Austin, TX, United States). Bubble plots were generated using ggplot2 R package with concentration (pg/ml).
Rhoades N.S., Hendrickson S.M., Prongay K., Haertel A., Gill L., Edwards R.A., Garzel L., Slifka M.K, & Messaoudi I. (2021). Growth faltering regardless of chronic diarrhea is associated with mucosal immune dysfunction and microbial dysbiosis in the gut lumen. Mucosal Immunology, 14(5), 1113-1126.
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