The following mAbs and reagents were used in this study: fluorescein isothiocyanate (FITC)-conjugated anti-CD3, phycoerythrin (PE)-Cy5-conjugated anti-CD161, FITC-conjugated anti-TCR γδ and PE-conjugated 6B11 (all from Becton Dickinson, San Diego, CA, USA), allophycocyanin (APC)-conjugated anti-TCR Vα7.2 (BioLegend, San Diego, CA, USA) and APC-Alexa Fluor 750-conjugated anti-CD3 (Beckman Coulter, Marseille, France). Cells were stained with combinations of appropriate mAbs for 20 minutes at 4°C. Stained cells were analyzed on a Navios flow cytometer using Kaluza software (Beckman Coulter, Brea, CA, USA).
Fitc conjugated anti tcr γδ
Manufactured by BD
Sourced in United States
The FITC-conjugated anti-TCR γδ is a laboratory reagent used to detect and identify gamma-delta T cells in flow cytometry analysis. It consists of a fluorescein isothiocyanate (FITC) molecule conjugated to an antibody that specifically binds to the gamma-delta T cell receptor (TCR γδ) on the surface of T cells.
Automatically generated - may contain errors
Lab products found in correlation
Pe cy5 conjugated anti cd161, by BD (4 mentions)
Navios flow cytometer, by Beckman Coulter (4 mentions)
Fitc conjugated anti cd3, by BD (3 mentions)
Pe conjugated 6b11, by BD (2 mentions)
Allophycocyanin apc conjugated anti tcr vα7, by BioLegend (2 mentions)
Apc alexa fluor 750 conjugated anti cd3, by Beckman Coulter (2 mentions)
Kaluza software, by Beckman Coulter (2 mentions)
Pe conjugated anti il 17, by BD (2 mentions)
Fitc conjugated anti ifn γ, by BD (2 mentions)
Pe cy7 conjugated anti tnf α, by BD (2 mentions)
Fitc conjugated mouse igg isotype, by BD (2 mentions)
Pe conjugated mouse igg isotype, by BD (2 mentions)
Apc conjugated anti tcr vα7, by BioLegend (2 mentions)
Pe conjugated anti ccr6, by BD (1 mentions)
Fitc conjugated anti cd107a, by BD (1 mentions)
Pe cy7 conjugated mouse igg isotype control, by BD (1 mentions)
Apc cy7 conjugated anti cd3, by BD (1 mentions)
Fitc conjugated annexin 5, by BD (1 mentions)
Pe conjugated anti cd3, by BD (1 mentions)
Pe conjugated anti cd69, by BD (1 mentions)
4 protocols using fitc conjugated anti tcr γδ
1
Flow Cytometry Immunophenotyping Panel
2
Multiparameter Flow Cytometry of Immune Cells
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The following mAbs and reagents were used in this study: fluorescein isothiocyanate (FITC)-conjugated anti-CD3, phycoerythrin (PE)-Cy5-conjugated anti-CD161, FITC-conjugated anti-TCR γδ and PE-conjugated 6B11 (all from Becton Dickinson, San Diego, CA, USA), allophycocyanin (APC)-conjugated anti-TCR Vα7.2 (BioLegend, San Diego, CA, USA) and APC-Alexa Fluor 750-conjugated anti-CD3 (Beckman Coulter, Marseille, France). Cells were stained with combinations of appropriate mAbs for 20 min at 4℃. Stained cells were analyzed on a Navios flow cytometer using Kaluza software (Beckman Coulter, Brea, CA, USA).
3
Multiparametric Flow Cytometry of Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following mAbs and reagents were used in this study: Allophycocyanin (APC)-Alexa Fluor 750-conjugated anti-CD3, phycoerythrin (PE)-Cy5-conjugated anti-CD161, fluorescein isothiocyanate (FITC)-conjugated anti-TCR γδ, FITC-conjugated anti-IFN-γ, PE-conjugated anti-IL-17, PE-Cy7-conjugated anti-TNF-α, PE-conjugated anti-CCR6, PE-conjugated anti-CCR9, PE-conjugated anti-CXCR3, PE-conjugated anti-CXCR6, FITC-conjugated anti-perforin, FITC-conjugated anti-granzyme, FITC-conjugated anti-CD107a, FITC-conjugated mouse IgG isotype, PE-conjugated mouse IgG isotype and PE-Cy7-conjugated mouse IgG isotype control (all from Becton Dickinson, San Diego, CA) and APC-conjugated anti-TCR Vα7.2 (BioLegend, San Diego, CA). Cells were stained with combinations of appropriate mAb for 20 minutes at 4°C. Stained cells were analyzed on a Navios flow cytometer using Kaluza software (Beckman Coulter, Brea, CA).
4
Immunophenotyping of T Cell Subsets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following mAbs and reagents were used in this study: Allophycocyanin (APC)-Cy7-conjugated anti-CD3, phycoerythrin (PE)-Cy5-conjugated anti-CD161 and fluorescein isothiocyanate (FITC)-conjugated anti-TCR γδ, FITC-conjugated anti-CD3, FITC-conjugated anti-IFN-γ, FITC-conjugated annexin V, PE-conjugated anti-CD3, PE-conjugated anti-IL-17, PE-Cy7-conjugated anti-TNF-α, PE-conjugated anti-CD69, FITC-conjugated mouse IgG isotype, PE-conjugated mouse IgG isotype and PE-Cy7-conjugated mouse IgG isotype control (all from Becton Dickinson, San Diego, CA); PE-conjugated anti-programmed death-1 (anti-PD-1; eBioscience, San Diego, CA) and APC-conjugated anti-TCR Vα7.2 (BioLegend, San Diego, CA). Cells were stained with combinations of appropriate mAbs for 20 minutes at 4°C. Stained cells were analyzed on a Navios flow cytometer using Kaluza software (version 1.1; Beckman Coulter, Brea, CA).
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