Sunitinib malate (SU), from Pfizer or LC Laboratories, and its vehicle were formulated as described before (Ebos et al, 2009 (link)). SU was administered by oral gavage (PO), once daily, at a dose of 60 mg/kg mouse weight. Vasculotide (VT): VT synthesized at the Sunnybrook Research Institute (Toronto, ON, Canada) was used in experiments described in Fig2 (A–D) and Supplementary Fig S5(A and B). VT synthesized by American Peptide (Sunnyvale, CA, USA) was used in experiments described in Fig5 . VT synthesized by Bachem UK Ltd was used in experiments described in Figs1 , 2 (E–H), 3 , 4 , 6–9, Supplementary Figs S2, and S5(C and D). Lyophilized product was reconstituted in PBS to 500 μg/500 μl stock aliquots and stored at −80°C. As needed, stock aliquots were diluted with PBS to a 250 ng/50 μl working concentration and stored in 0.4–1 ml aliquots at −80°C. VT (at a standard dose of 250 ng/mouse) or its vehicle (PBS) was administered to mice by intraperitoneal (IP) injections every other day.
Sunitinib malate
Manufactured by Pfizer
Sourced in United States
Sunitinib malate is a small molecule that inhibits multiple receptor tyrosine kinases. It is used as a reference standard in analytical testing and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Visudyne, by Novartis (1 mentions)
Gemcitabine, by Merck Group (1 mentions)
Docetaxel, by Merck Group (1 mentions)
Fluorescein isothiocyanate labeled phalloidin, by Merck Group (1 mentions)
Matrigel, by BD (1 mentions)
Sc 236, by Cayman Chemical (1 mentions)
Mk2206, by Merck Group (1 mentions)
5 protocols using sunitinib malate
1
Sunitinib and Vasculotide Administration
2
Photophysical Properties of Sunitinib and Visudyne
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Stock solutions of the free form of sunitinib (sunitinib malate, Pfizer Inc., New York, NY, USA) were prepared in 100% DMSO (Sigma-Aldrich). Dilutions were made in 0.9% NaCl under continuous stirring. Visudyne (Novartis Pharma Inc.) was dissolved in 5% glucose and diluted in NaCl. Absorption spectra were recorded with a two-beam Varian Cary UV-Vis-NIR 500 Scan spectrophotometer in 1-cm-long quartz cuvettes (Suprasil, Hellma, Müllheim, Germany) between 350 and 800 nm with an average scan speed of 600 nm/min at 20 °C. The steady-state luminescence spectra were recorded with a luminescence spectrometer (Perkin-Elmer, model LS50B, Waltham, MA, USA).
3
Anti-miRNA Oligonucleotides and Chemotherapeutics
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Anti-microRNA oligonucleotides (AMOs) against miR-221, miR-222, miR-21, and miR-10b and scrambled oligonucleotides (control), as well as 5′-fluorescein-labeled oligonucleotides for confocal microscopy and digoxigenin (DIG)-labeled oligonucleotides for miR-21 detection, were all purchased from Exiqon (Vedbaek, Denmark) as miRCURY locked nucleic acids (LNA™).
The chemotherapeutic drugs docetaxel and gemcitabine were purchased from Sigma-Aldrich, and stock solutions were prepared in distilled water and subsequently stored at −20°C and at room temperature, respectively. Sunitinib malate (Sutent®) was kindly offered by Pfizer, Inc. (New York, NY, USA), and the stock solutions were prepared in dimethyl sulfoxide (Sigma-Aldrich) and stored at −20°C.
The chemotherapeutic drugs docetaxel and gemcitabine were purchased from Sigma-Aldrich, and stock solutions were prepared in distilled water and subsequently stored at −20°C and at room temperature, respectively. Sunitinib malate (Sutent®) was kindly offered by Pfizer, Inc. (New York, NY, USA), and the stock solutions were prepared in dimethyl sulfoxide (Sigma-Aldrich) and stored at −20°C.
4
Sunitinib and Peptide HM-3 Combination Protocol
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Sunitinib malate (Sutent, Pfizer) was suspended in solvent 1, which contained carboxymethylcellulose sodium (0.5% w/v), NaCl (1.8% w/v), Tween 80 (0.4%, w/v), benzyl alcohol (0.9 w/v), and deionized water (added to final volume) adjusted to pH 6.0. Peptide HM-3 (Ile-Val-Arg-Arg-Ala-Asp-Arg-Ala-Ala-Val-Pro-Gly-Gly-Gly-Gly-Arg-Gly-Asp) was synthesized by GL Biochem Ltd. (Shanghai, China) and had a purity in excess of 99%. Other reagents included Matrigel (BD Biosciences), Fluorescein Isothiocyanate labeled Phalloidin (Sigma-Aldrich), Rhotekin RBD and PAK-1 PBD agarose conjugates (Millipore). The antibodies used for immunohistochemistry included: anti-CD31 polyclonal antibodies (sc-28188, Santa Cruz Biotech), anti-CD34 polyclonal antibodies (ZA-0550). Docetaxel was from Jiangsu Hengrui Pharmaceutical Co. LTD in China.
5
Preparation and Storage of Pharmacological Agents
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ASA or aspirin (acetylsalicylic acid) was purchased from Sigma and diluted in 0.9% NaCl solution with dimethyl sulfoxide (DMSO). The final concentration of DMSO in the medium was 1/1000 (v/v). The solution was freshly prepared. SC-236 was purchased from Cayman Chemical (Ann Arbor, MI, USA) and was dissolved in ethanol with phosphate buffered saline pH 7.6 (PBS) in a 1:4 solution of ethanol:PBS, which was stored at −20°C. Sunitinib Malate was obtained from Pfizer (Pfizer Inc., New York, NY, USA) and was suspended in carboxymethylcellulose (CMC) solution (0.5% CMC, 1.8% NaCl, 0.4% Tween 80, and 0.9% benzyl alcohol in distilled water) for in vivo experiments. Drug aliquots were prepared once weekly and kept in the dark at 4°C. DC101 was obtained from ImClone Systems (Branchburg, NJ, USA) and was diluted in PBS. Aliquots were stored at −20°C. PGE2 was bought from Cayman Chemical, dissolved in DMSO and stored at −20°C. The Akt inhibitor MK-2206 was purchased from Merck Millipore (Darmstadt, Germany), dissolved in PBS with 20% ethanol and stored at −20°C.
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