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2 protocols using agvpglgvg

1

Synthetic Elastin Peptides for Cell Research

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Synthetic elastin peptides (VGVAPG, AGVPGLGVG, GRKRK and TAMRA-AGVPGLGVG) were purchased from Proteogenix. Mouse anti-MMP-2 and anti-uPA antibodies were from Calbiochem. Y27632, blebbistatin, U0126, PD150606, lactose, chondroitin sulphate, nifedipine and EDTA were from Sigma-Aldrich. EGCG was purchased from Enzo Life Sciences. Rabbit anti-Hsp90, anti-cleaved caspase-3, anti-integrin αV, anti-p-ERM, mouse anti-αvβ3 and anti-αvβ5 integrin antibodies were from Ozyme. Rabbit anti-RPSA, anti-MMP-14, anti-calpain1, anti-ROCK2, anti-myosin light chain kinase, mouse anti-RPSA, anti-RhoA, anti-ROCK1 and mouse IgM isotype control antibodies were purchased from Abcam. Rabbit anti-p-LIMK-and goat anti-cofilin and anti-actin were from Santa Cruz. Anti-integrin αvβ3 antibody was purchased from Millipore. Annexin-5 alexa fluor® 568, CellTrace Calcein Red-Orange, AM and DiOC183 were from Invitrogen.
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2

Hsp90-Targeting Elastin Peptides Modulate

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Synthetic elastin peptides (VGVAPG, AGVPGLGVG and GRKRK) were purchased from Proteogenix (Schiltigheim, France). Matrigel was obtained from Becton-Dickinson (Le Pont de Claix, France). Plasminogen, mouse anti-MMP-2 and anti-uPA antibodies were obtained from Calbiochem (distributed by VWR, Strasbourg, France). Geldanamycin was obtained from Invivogen (Toulouse, France). Radicicol was purchased from Tebu-bio (Le Perray-en-Yvelines, France). Rabbit monoclonal anti-Hsp90 was from Ozyme (Saint Quentin Yvelines, France). Mouse anti-Hsp90 was purchased from Abcam (Paris, France). Goat anti-actin was from Santa Cruz (Heidelberg, Germany). Recombinant Hsp90α protein was from Enzo Life Sciences (Villeurbanne, France).
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