Creatinine kit

Manufactured by Cayman Chemical

Sourced in United States

The Creatinine kit is a laboratory reagent used to measure the concentration of creatinine in various biological samples, such as serum, plasma, or urine. Creatinine is a breakdown product of creatine phosphate in muscle and is a commonly used indicator of kidney function.

Automatically generated - may contain errors

Lab products found in correlation

Mouse albumin specific elisa, by Fortis Life Sciences (2 mentions) Sandwich elisa kit, by Cayman Chemical (1 mentions) Dxc800, by Beckman Coulter (1 mentions)

Close spelling variants of this product

Creatinine urinary/serum Colorimetric Assay Kits 96-well Creatinine Assay Kit Mouse/rat creatinine ELISA kit Creatinine (serum) Colorimetric Assay Kit Creatinine assay kit Mouse creatinine kit Creatinine (urinary) Colorimetric Assay Kit Cayman’s Creatinine Assay Kit Creatinine (urinary) assay kit Creatinine Colorimetric Assay Kit

7 protocols using creatinine kit

Oxidative Damage Biomarkers in Urine and WBC

Check if the same lab product or an alternative is used in the 5 most similar protocols

The urinary RNA and DNA oxidative damage products 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyGua), 8-oxo-7,8-dihydroguanine (8-oxoGua), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), and 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodGuo) was simultaneously measured for participants employing electrospray tandem mass spectrometry detection (MS/MS) in multiple reaction monitoring (MRM) mode on a Finnigan TSQ 7000 triple quadrupole mass spectrometer (Thermo Electron Corporation, San Jose, CA). The urinary biomarkers were normalized to the concentration of creatinine, which was assessed using a creatinine kit (Cayman Chemical, Ann Arbor, MI).
RNA and DNA oxidative damage levels to peripheral blood WBC was measured in lysed cells in 4.5 mL of 3 M GTC buffer (0.2 wt.% N-L-Sarcosine, 20 mM tris [pH 7.5]) containing 10 mM of the freshly dissolved metal chelator deferoxamine meylate (DFOM) during homogenization using a Potter-Elvehjem homogenizer. RNA/DNA hydrolysis was performed using Nuclease P1 and alkaline phosphatase, and 8- oxoGuo/ guanosine (Guo) and 8-oxodGuo/2-deoxyguanosine (dGuo) ratios were determined using HPLC-ECD with a Coulochem III electrochemical detector (ESA Inc., Chbelmsford, MA), as described previously[52 (link)].

Extermann M., Leeuwenburgh C., Samiian L., Sehovic M., Xu J., Cubitt C., Jacobsen P.B., Pahor M., Grobmyer S.R, & Manini T.M. (2016). Impact of chemotherapy on medium-term physical function and activity of older breast cancer survivors, and associated biomarkers. Journal of geriatric oncology, 8(1), 69-75.

+ Open protocol

+ Expand

Oxidative Stress Biomarkers in Urine

Check if the same lab product or an alternative is used in the 5 most similar protocols

Urine samples were collected in metabolic cages for 2 h just before killing and frozen at −80°C until analysis. MDA and NOx levels in urine were measured by an enzyme-linked immunosorbent assay (ELISA) kit (Cayman, Ann Arbor, MI, USA) according to manufacturer’s instructions. NOx and MDA levels were expressed as micromoles. Urinary 8-OHdG levels were determined by a sandwich ELISA kit (Cayman). The concentration of creatinine in the urine samples was determined using a creatinine kit purchased from Cayman chemicals. Data were shown as the ratio of 8-OHdG to creatinine (Inano and Onoda, 2002 (link)).

Kim S.H., Lee I.C., Ko J.W., Moon C., Kim S.H., Shin I.S., Seo Y.W., Kim H.C, & Kim J.C. (2015). Diallyl Disulfide Prevents Cyclophosphamide-Induced Hemorrhagic Cystitis in Rats through the Inhibition of Oxidative Damage, MAPKs, and NF-κB Pathways. Biomolecules & Therapeutics, 23(2), 180-188.

+ Open protocol

+ Expand

Urine Collection and Albumin Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

A single point mid-stream specimen of urine was collected from study participants after an overnight fast, between 8:00 am and 10:00 am. After centrifugation to remove any debris, urine was fractionated and stored in polycarbonate tubes at -80°C until required for analyses. Urine was diluted (10-20X) and levels of creatinine determined using the improved Jaffe method using picrate using creatinine (0–15 mg/dL) as a standard (Creatinine kit, # 500701, Cayman Chemical Company, Ann Arbor, MI). Urine albumin was quantified using size exclusion chromatography (HP1050) on a Zorbax GF-250 column (4.6 x 250 mm) using 0.1 PBS (pH 7.0) at a flow rate of 0.5 mL/min. The column was calibrated with thyroglobulin (670 kDa), gamma globulin (158 kDa), ovalbumin (44 kDa), myoglobulin (17 kDa), and vitamin B-12 (1.35 kDa) and levels of albumin calculated (mg/mL).

Castor K.J., Shenoi S., Edminster S.P., Tran T., King K.S., Chui H., Pogoda J.M., Fonteh A.N, & Harrington M.G. (2020). Urine dicarboxylic acids change in pre-symptomatic Alzheimer’s disease and reflect loss of energy capacity and hippocampal volume. PLoS ONE, 15(4), e0231765.

+ Open protocol

+ Expand

CKD Mouse Model via Subtotal Nephrectomy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Male C57BL/6 mice (8 weeks old) underwent subtotal nephrectomy to induce CKD by a method described previously.³² Briefly, mice were anesthetized with ketamine (125 mg/kg body wt) and xylazine (6.4 mg/kg body wt). The left kidney was decapsulated, and approximately three‐quarters was removed. During the recovery, mice received buprenorphine (0.1–2.5 mg/kg body wt). A week later, the right kidney was removed. Sham‐operated control mice were pair‐fed with CKD mice using the same diets (40% protein chow). CKD was confirmed by serum creatinine (average 2.2 ± 0.3 mg/dl in CKD groups) and BUN (average 82.1 ± 6.3 mg/dl in CKD groups). Serum creatinine level was determined using a creatinine kit (Cayman Chemicals). The TA muscles of mice with TLR13 silenced were injected with 0.01 nmol of SMARTpool siRNA targeting TLR13 (GE Dharmacon) in 20 ml of phosphate‐buffered saline; control mice received the same quantity of scrambled siRNA.

Gu L., Wang Z., Zhang Y., Zhu N., Li J., Yang M., Wang L, & Rong S. (2022). TLR13 contributes to skeletal muscle atrophy by increasing insulin resistance in chronic kidney disease. Cell Proliferation, 55(3), e13181.

+ Open protocol

+ Expand

Urinary Albumin-Creatinine Ratio Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice urinary albumin and creatinine were measured using mouse albumin-specific ELISA (Bethyl Laboratories Inc, Montgomery, TX, USA) and creatinine kits (Cayman Chemical, Ann Arbor, MI, USA) respectively, according to the manufacturer's instructions. The results were presented as albumin/creatinine ratio (ACR, μg/mg).

Yu C., Zhang H., Liu S., Li R., Zhao X., Chen Y., Li Z., Ma J., Wang W., Ye Z., Liang X., Zhang L, & Shi W. (2023). Flot2 acts as a novel mediator of podocyte injury in proteinuric kidney disease. International Journal of Biological Sciences, 19(2), 502-520.

+ Open protocol

+ Expand

Plasma Biomarker Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma samples were acquired by centrifugation of the blood samples at 3,000 × g and 4°C, and blood urea nitrogen (BUN) was measured using a Beckman Coulter automated biochemical analyzer (DXC800, Beckman Coulter Inc., Pasadena, CA). Urinary albumin or urinary and serum creatinine (Scr) levels were, respectively, measured using mouse albumin-specific enzyme-linked immunosorbent assay kits (Bethyl Laboratories Inc., Montgomery, TX, USA) and creatinine kits (Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer’s instructions.

Zhang X., Zhang L., Wen Y., Zhang M., Liu S, & Xiao H. (2023). Vitamin D Ameliorates Podocyte Injury by Enhancing Autophagy Activity in Diabetic Kidney Disease. Kidney & Blood Pressure Research, 48(1), 314-325.

+ Open protocol

+ Expand

Murine Diabetic Nephropathy Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Animal care and experiments were performed in accordance with the ARRIVE guidelines,^{40 (link)} and were approved by the Ethics Committee for animal research of Guangdong General Hospital. Six male C57BL/KsJ db/db mice and 5 age-matched wild-type (BKS) mice were purchased from Model Animal Research Center of Nanjing University. Thirty-six male BALB/c mice aged 8 weeks were purchased from Center of Laboratory Animal Science of Guangdong. Mice were anaesthetized (ketamine, 70 mg/kg, intraperitoneally (i.p.)) before killed and kidney tissue were collected. Mice urinary albumin and creatinine were measured using mouse albumin-specific ELISA (Bethyl Laboratories Inc, Montgomery, TX, USA) and creatinine kits (Cayman Chemical, Ann Arbor, MI, USA) respectively, according to the manufacturer's instructions. Proteinuria was expressed as the ratio of albumin to creatinine.

Huang Z., Zhang L., Chen Y., Zhang H., Zhang Q., Li R., Ma J., Li Z., Yu C., Lai Y., Lin T., Zhao X., Zhang B., Ye Z., Liu S., Wang W., Liang X., Liao R, & Shi W. (2016). Cdc42 deficiency induces podocyte apoptosis by inhibiting the Nwasp/stress fibers/YAP pathway. Cell Death & Disease, 7(3), e2142-.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!