Anti c fos

Western blotting was performed as previously described,^{35 (link)} using anti-CDK5, anti-FAK, anti-p-FAK (Ser732), anti-PAK1, anti-p-PAK1 (Thr212), anti-ERK5, anti-p-ERK5 (Thr218/Tyr220) (Abcam), anti-p-ERK5 (Thr732), anti-p35 (Cell Signaling Technology, Danvers, MA, USA; anti-p-ERK5 Thr732 was custom-made from CST), anti-c-fos, anti-c-jun (Bioworld Technology, Louis Park, MN, USA), anti-c-myc, anti-VEGFA and anti-MMP1 antibodies (Proteintech, Chicago, IL, USA). Loading control was used with a mouse anti-β-Actin monoclonal antibody (Proteintech).

RAW264.7 cells were obtained from the American Type Culture Collection (Rockville, MD, USA). WKYMVm and WRWWWW (WRW4) with purity > 95% was synthesized by GL Biochem and was dissolved in acetonitrile to prepare stock concentrations of 0.1, 1, 2, 5 and 10 μmol/L. Dulbecco's Modified Eagle's Medium (DMEM), alpha minimum essential medium (α‐MEM) and foetal bovine serum (FBS) were purchased from Invitrogen‐Gibco. RANKL and soluble mouse recombinant M‐CSF were obtained from R&D Systems. The Acid Phosphatase, Leukocyte (TRAP) assay kit and Actin Cytoskeleton and Focal Adhesion (FAK) Staining Kit were purchased from Sigma (Sigma‐Aldrich) and Merck, respectively. The Cell Counting Kit‐8 (CCK‐8) was purchased from Beyotime Biotechnology. Anti‐phospho‐NF‐kBp65, anti‐NF‐kBp65, anti‐STAT3, anti‐phospho‐STAT3 (Ser727), anti‐CTSK, anti‐c‐Fos, anti‐NFATc1, anti‐CD9, anti‐gp130, anti‐IL6 and anti‐ß‐actin antibodies were purchased from Bioworld Technology. Mouse IL‐1β, IL‐6 and TNF‐α ELISA kits were procured from Novus.