Icw 3 000 water purifier for ion chromatograph

Immediately after euthanasia on day 16 and 29, the gizzard, ileum, and ceca were removed intact from 2 birds per pen. The pH was measured and recorded in duplicate. In brief, a digital pH meter (Mettler-Toledo, UK) with a spear tip piercing pH electrode (Sensorex, Garden Grove, CA) was directly inserted into the digesta in the lumen of the proximal gizzard (proventricular opening), ileum, and ceca of the same bird, while avoiding direct contact of the pH electrode with the gut wall. Once the 2 readings per sample were recorded, the probe was rinsed with ultra-pure water (ICW 3,000 water purifier for ion chromatograph; Millipore, Burlington, MA). The mean of the 2 readings per site of the tract was then calculated and recorded. The digesta of the ileum and ceca from the 2 birds were sampled to determine the digestibility of nutrients and bacterial quantification, respectively.

Immediately posteuthanasia on the day 16, the gizzard, ileum, and ceca were removed intact from 2 birds per pen. A digital pH meter (Mettler-Toledo, UK) with a spear tip piercing pH electrode (Sensorex, Garden Grove, CA) was directly inserted into the digesta in the lumen of the proximal gizzard (proventricular opening), ileum, and ceca of the same bird, while avoiding direct contact of the pH electrode with the gut wall. The pH was measured and recorded in duplicate. Once all the readings were taken, the probe was rinsed with ultra-pure water (ICW 3000 water purifier for ion chromatograph; Millipore, Burlington, MA). The mean of the 2 readings per site of the tract was then calculated and recorded. The digesta of the ileum and ceca from the 2 birds were sampled to determine the digestibility of nutrients and bacterial quantification, respectively.