Ff01 357 44 25

Manufactured by IDEX Corporation

Sourced in United Kingdom

The FF01-357/44-25 is a lab equipment product manufactured by IDEX Corporation. It is a precision instrument designed for specialized laboratory applications. The core function of this product is to perform specific tasks within a controlled laboratory environment.

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Lab products found in correlation

Ff01 280 20 25, by IDEX Corporation (2 mentions) Aca254 200 uv, by Thorlabs (2 mentions) C9100 13, by Hamamatsu Photonics (1 mentions) Ff01 520 35 25, by IDEX Corporation (1 mentions) Q505lp, by Chroma Technology (1 mentions) Lmu 10x uvb, by Thorlabs (1 mentions)

3 protocols using ff01 357 44 25

Quantifying Microbial Cell Dynamics

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Microdroplet generation was monitored through an objective (UPlanApo 20 ×/0.70 NA, Olympus) by a high-speed camera (LRH2500XE, Digimo) mounted on an inverted microscope (IX-71, Olympus).
Microbial cells were observed using an inverted microscope (IX71, Olympus) with an oil-immersion objective (UPlanApo 40 ×/1.00 NA Oil Iris, Olympus), a xenon lamp and filter sets to observe fluorescence from 4′,6-diamidino-2-phenylindole (DAPI) [excitation filter, FF01-357/44-25 (Semrock); dichroic mirror, FF409-Di03-25 × 36 (Semrock); emission filter, FF02-447/60-25 (Semrock)] and fluorescein [excitation filter, FF01-472/30-25 (Semrock); dichroic mirror, Q505LP (Chroma Technology); emission filter, FF01-520/35-25 (Semrock)]. The bright-field and fluorescence images were captured using an electron multiplying CCD camera (C9100-13, Hamamatsu Photonics). Microbial cell concentrations were estimated by DAPI staining (3 μg/mL, Polysciences) and direct epifluorescence microscopic counting.

Nakamura K., Iizuka R., Nishi S., Yoshida T., Hatada Y., Takaki Y., Iguchi A., Yoon D.H., Sekiguchi T., Shoji S, & Funatsu T. (2016). Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform. Scientific Reports, 6, 22259.

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Microfluidic Fluorescence Detection Setup

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The schematic of the optical layout is shown in Fig. 2b. The sample was excited using either a 280 nm LED (Thorlabs M280L3, UK) or a 365 nm LED (Thorlabs M365L2, UK) light source with a flip mirror used to switch between the two sources. The light from either of the LEDs was passed through an aspherical lens of focal length 20 mm to get a collimated output beam. The beam was passed through a dichroic filter cube, which consisted of an excitation filter (Semrock FF01-280/20-25) and a dichroic mirror (Semrock FF310-Di01-25 × 36). The light reflected by the dichroic mirror was then focussed onto the sample flowing in the microfluidic device by an infinity corrected UV objective lens (Thorlabs LMU-10X-UVB, UK) of numerical aperture NA = 0.25.The emitted fluorescent light from the sample was collected through the same objective and an emission filter (Semrock FF01-357/44-25 for a characteristic tryptophan, FF01-302/10-25 for a characteristic tyrosine and FF01-452/45-25 for a characteristic lysine signal) with an air-spaced achromatic doublet lens of focal length 20 mm (Thorlabs ACA254-200-UV) focussing it onto the camera (Rolera EMC2). All the optics used in the set-up were made out of fused silica for high transmission in the UV region.^{28 (link)}

Zhang Y., Wright M.A., Saar K.L., Challa P., Morgunov A.S., Peter Q.A., Devenish S., Dobson C.M, & Knowles T.P. (2021). Machine learning-aided protein identification from multidimensional signatures. Lab on a Chip, 21(15), 2922-2931.

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Fluorescence Detection in Microfluidics

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The schematic of the optical layout is shown in Figure 2b. The sample was excited using either a 280 nm LED (Thorlabs M280L3, UK) or a 365 nm LED (Thorlabs M365L2, UK) light source with a flip mirror used to switch between the two sources. The light from either of the LEDs was passed through an aspherical lens of focal length 20 mm to get a collimated output beam. The beam was passed through a dichroic filter cube, which consisted of an excitation filter (Semrock FF01-280/20-25) and a dichroic mirror (Semrock FF310-Di01-25x36). The light reflected by the dichroic mirror was then focussed onto the sample flowing in a microfluidic chip by an infinity corrected UV objective lens (Thorlabs LMU-10X-UVB, UK) of numerical aperture NA = 0.25. The emitted fluorescent light from the sample was collected through the same objective and an emission filter (Semrock FF01-357/44-25 for a charactersitic tryptophan, FF01-302/10-25 for a charactersitic tyrosine and FF01-452/45-25 for a charactersitic lysine signal) with an air-spaced achromatic doublet lens of focal length 20 mm (Thorlabs ACA254-200-UV) focussing it onto the camera (Rolera EMC2). All the optics used in the set-up were made out of fused silica for high transmission in the UV region. [28, 29] 3/13

Zhang Y., Wright M.A., Saar K.L., Challa P.K., Morgunov A.S., Peter Q., Devenish S.R.A., Dobson C.M., & Knowles T.P.J. (2020). Machine learning aided top-down proteomics on a microfluidic platform.

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