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Pnase

Manufactured by Merck Group
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PNase is a laboratory instrument used for the purification and isolation of nucleic acids, such as DNA and RNA. It functions by breaking down phosphodiester bonds in the nucleic acid backbone, facilitating the separation and extraction of these biomolecules from complex samples.

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Lab products found in correlation

Chabc, by Merck Group (2 mentions) Chabc, by Seikagaku (1 mentions)

3 protocols using pnase

1

Stereotaxic Chondroitinase Delivery in Rat Perirhinal Cortex

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Protease-free ChABC (Seikagaku, Japan) or penicillinase (P-nase; Sigma-Aldrich, UK) as a non-relevant control enzyme was dissolved in 0.1% BSA to 50 U/ml of concentration and filtered through a 0.2 micron filter. Six injections in the PRh (3 per hemisphere, 0.5 μl with a speed of 0.2 μl/min) were performed stereotaxically under isoflurane anesthesia with a 10 μl Hamilton syringe and a 33 gauge needle at the following sites (in mm from bregma and the surface of skull): 1. anterior–posterior (AP): − 1.8; lateral (L): ± 4.6; ventral (V): − 4.4, 2. AP: − 2.8; L: ± 4.8; V: − 4.3 and 3. AP: − 3.8; L: ± 4.8; V: − 3.8. The needle remained in place at the injection site for 3 min before being slowly withdrawn over 2 min.
Yang S., Cacquevel M., Saksida L.M., Bussey T.J., Schneider B.L., Aebischer P., Melani R., Pizzorusso T., Fawcett J.W, & Spillantini M.G. (2015). Perineuronal net digestion with chondroitinase restores memory in mice with tau pathology. Experimental Neurology, 265, 48-58.
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2

Retrograde Labeling of CA2 Granule Cells

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To label granule cells projecting to the CA2, unilateral injections of the retrograde virus rAAV-EF1a-mCherry-WPRE-hGHpA virus (AAV2-retro, made by the Princeton Viral Core, titer: 1×1013) were made in the CA2. To explore whether CA2 PNNs participate in social memory, bilateral injections of chondroitinase ABC (chABC, 50 U/ml, Sigma), an enzyme that temporarily degrades PNNs by digesting the sulfated sugar chains responsible for PNN glycoprotein function, or the control enzyme penicillinase (PNase, 50 U/ml, Sigma) were made in the CA2. CA2 coordinates were −1.82 AP, +/−2.15 ML, and −1.7 DV for control mice and −1.7 AP, +/−2.6 ML, and −1.4 DV for BTBR mice. See Supplementary Methods.
Cope E.C., Zych A.D., Katchur N.J., Waters R.C., Laham B.J., Diethorn E.J., Park C.Y., Meara W.R, & Gould E. (2021). Atypical perineuronal nets in the CA2 region interfere with social memory in a mouse model of social dysfunction. Molecular psychiatry, 27(8), 3520-3531.
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3

Retrograde Labeling of CA2 Granule Cells

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To label granule cells projecting to the CA2, unilateral injections of the retrograde virus rAAV-EF1a-mCherry-WPRE-hGHpA virus (AAV2-retro, made by the Princeton Viral Core, titer: 1×1013) were made in the CA2. To explore whether CA2 PNNs participate in social memory, bilateral injections of chondroitinase ABC (chABC, 50 U/ml, Sigma), an enzyme that temporarily degrades PNNs by digesting the sulfated sugar chains responsible for PNN glycoprotein function, or the control enzyme penicillinase (PNase, 50 U/ml, Sigma) were made in the CA2. CA2 coordinates were −1.82 AP, +/−2.15 ML, and −1.7 DV for control mice and −1.7 AP, +/−2.6 ML, and −1.4 DV for BTBR mice. See Supplementary Methods.
Cope E.C., Zych A.D., Katchur N.J., Waters R.C., Laham B.J., Diethorn E.J., Park C.Y., Meara W.R, & Gould E. (2021). Atypical perineuronal nets in the CA2 region interfere with social memory in a mouse model of social dysfunction. Molecular psychiatry, 27(8), 3520-3531.
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