Mouse anti human glyceraldehydes 3 phosphate dehydrogenase gapdh

Reagents. Fetal bovine serum (FBS) and DMEM were purchased from Gibco (San Francisco, CA, USA). Primary antibodies: rabbit anti-human RSK2 was from Bioworld (USA). Rabbit anti-human total c-Fos, rabbit anti-human total AKT, rabbit anti-human total mTOR, and phosphorylated ERK, phosphorylated AKT, phosphorylated mTOR were from Cell Signaling Technology (Boston, MA, USA). Rabbit antihuman Bax, Bcl2, caspase-3, rabbit anti-human Ki67 nuclear antigen, mouse anti-human PCNA, mouse anti-human β-actin and mouse anti-human glyceraldehydes-3-phosphate dehydrogenase (GAPDH) were from Santa Cruz Biotechnology (San Francisco, CA, USA). Horseradish peroxidase-conjugated goat anti-rabbit and goat anti-mouse secondary antibodies were from Zhong Shan Golden Bridge Biotechnology (Beijing, China).
shRNA. The RSK2 shRNA were designed and synthesized by Genechem Co. Ltd. (Shanghai, China) and the sequences targeting RSK2 were 5'-TGCCACAATACCAACTAAA-3'. A non-specific scrambled shRNA with a sequence of 5'-TTCTC CGAACGTGTCACGT-3' was used as a negative control.