Ferrous sulphate heptahydrate

Commercial O. vulgare essential oil (CO) was purchased from Botanicessence (Product of Spain) (Bangkok, Thailand). Carvacrol, α-tocopherol, 2,2′-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6, tripyridyl-s-triazine (TPTZ), hydrochloric acid (HCl), acetic acid (CH₃COOH), linoleic acid, hyaluronidase from bovine testes, sodium chloride (NaCl), collagenase from Clostridium histolyticum, N-[3-(2-furyl)acryloyl]-Leu-Gly-Pro-Ala (FALGPA), elastase from porcine pancreas, N-succinyl-Ala-Ala-Ala-p-nitroanilide (AAAVPN), sodium phosphate monobasic dihydrate (NaH₂PO₄.2H₂O), and sodium phosphate dibasic dihydrate (Na₂HPO₄.2H₂O) were purchased from Sigma-Aldrich (St. Louis, MO, USA); α-Ascorbic acid was purchased from Asia Pacific Specialty Chemicals Limited (New South Wales, Australia). Anhydrous sodium sulfate (Na₂SO₄), ferric chloride hexahydrate (FeCl₃.6H₂O), ferrous sulphate heptahydrate (FeSO₄.7H₂O), and ammonium thiocyanate (NH₄SCN) were purchased from Loba Chemie (Boisar, Tarapur, India). Bovine serum albumin was purchased from Merck (Darmstadt, Germany). Tricine was purchased from Bio-Rad Laboratories (Richmond, CA, USA). Sodium acetate trihydrate (CH₃COONa.3H₂O), calcium chloride dihydrate (CaCl₂.2H₂O), sodium hydroxide (NaOH), dimethyl sulfoxide (DMSO), dichloromethane, methanol, and ethanol were analytical grade and purchased from RCI Labscan Co., Ltd. (Bangkok, Thailand).