6540 q tof instrument

Manufactured by Agilent Technologies

The 6540 Q-TOF instrument is a high-resolution mass spectrometer designed for accurate mass measurement and identification of unknown compounds. It combines a quadrupole mass analyzer with a time-of-flight (TOF) mass analyzer to provide precise mass data and fragmentation information.

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Lab products found in correlation

Am 500 spectrometer, by Bruker (1 mentions)

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6540 Q-TOF (13 citations) Agilent 6540 Q-TOF instrument (2 citations) 1290 UPLC/6540 Q-TOF mass instrument (2 citations)

2 protocols using 6540 q tof instrument

Targeted LC-MS/MS Quantification of Peptides

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Targeted LC-MS/MS was run on an Agilent 6540 Q-TOF instrument with AdvanceBio peptide Map column (2.1×150 mm 2.7 μm). 6 peptides (SALVNEYNVDASR, GPLDAAPAYGVK, VTTSGFDGSK, VEENIGQPLSPYAITK, AATMIGLTPQAPS and LFNATAGGYAK) were quantified. The internal control spike was approximately 10 pmol. The collision energy and other MS parameters were optimized before the sample run. The MS/MS acquisition time was set to 400 ms for all ions. Further information about the targeted LC-MS/MS analysis is provided in the online supplemental file that accompanies this article.

Wang H., Drake S.K., Yong C., Gucek M., Tropea M., Rosenberg A.Z., Dekker J.P, & Suffredini A.F. (2016). A Novel Peptidomic Approach to Strain Typing of Clinical Acinetobacter baumannii Isolates Using Mass Spectrometry. Clinical chemistry, 62(6), 866-875.

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Synthesis and Characterization of Organic Compounds

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All the reagents and solvents were purchased from commercial suppliers (Beijing InnoChem Science & Technology Co., Ltd., Beijing, China and Sinopharm Chemical Reagent Co., Ltd., Beijing, China). All reactions were monitored using thin-layer chromatography (TLC) run on silica gel glass plates (Qingdao Broadchem Industrial, Qingdao, China). ¹H and ¹³C NMR spectroscopy were recorded using a Bruker AM-500 spectrometer with temperature control at 21–23 °C, using DMSO-d6 or CDCl₃ as the solvent and tetramethyl silane (TMS) as the internal reference. In the spectra, the chemical shifts (δ) were given in parts per million (ppm). High-resolution mass spectra (HRMS) were determined with an Agilent 6540 QTOF instrument.

Feng T., Liu Q., Xu Z.Y., Li H.T., Wei W., Shi R.C., Zhang L., Cao Y.M, & Liu S.Z. (2023). Design, Synthesis, Herbicidal Activity, and Structure–Activity Relationship Study of Novel 6-(5-Aryl-Substituted-1-Pyrazolyl)-2-Picolinic Acid as Potential Herbicides. Molecules, 28(3), 1431.

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