Dionex utimate 3000 series hplc system

Chromatographic analysis was performed on a Dionex Utimate 3000 Series HPLC system (Thermo Scientific, Shanghai, China) equipped with a quaternary solvent delivery system and a column temperature controller. All the samples were analyzed at a column temperature of 30 °C on a Diamosil-C₁₈ column (4.6 mm × 250 mm, 5 μm, Dema, Beijing, China). The mobile phase consisted of water with 0.1% formic acid solution (eluent B), and acetonitrile (eluent A) using a gradient elution mode of 10% A at 0–3 min, 10–15% A at 3–5 min, 15–26% A at 5–60 min, 26–66% A at 60–110 min, 66% A at 110–120 min. The flow rate was 1.0 mL/min, and the injection volume was 10 μL.
A mass spectrometer (Thermo Scientific, LTQ-Orbitrap XL, Shanghai, China) was applied to MS detection. The operation conditions were as follows: capillary temperature, 350 °C; apci vaporizer temperature, 300 °C; sheath gas flow, 30 L/min; aux gas flow, 10 L/min; source voltage, 3 kV; capillary voltage, −35 V; Tube Lens, −110 V. Mass spectra were recorded across the range of m/z 50–2000. Thermo Workstation Acquisition Software Xcalibur Version 2.2 and UHPLC analysis Software Chromeleon Version 7.0 were utilized for system control, data acquisition and data processing.

Fingerprint analysis and quantitative analysis were conducted on a Dionex Utimate 3000 Series HPLC system (Thermo Fisher, Shanghai, China) equipped with a quaternary solvent delivery system, an ultraviolet detector with full wavelength scanning, and a column temperature controller.
All samples of fingerprint analysis and quantitative analysis were analyzed at a column temperature of 30 °C on a Thermo Scientific-C18 column (4.6 × 250 mm, 5 μm, Thermo Fisher, Shanghai, China). The mobile phase consisted of 0.1% phosphoric acid aqueous solution (eluent A) and acetonitrile (eluent B), the gradient elution mode was set as follows: 2–7% B at 0–6 min, 7–9% B at 6–10 min, 9% B at 10–15 min, 9–13% B at 15–35 min, 13% B at 35–40 min, 13–19% B at 40–54 min, 19–29% B at 54–66 min, 29–80% B at 66–103 min, and 80% B at 103–108 min. The injection volume was 10 μL and the flow rate was 1.0 mL/min.