Cd38 pecf594

Manufactured by BD

CD38-PECF594 is a fluorescently-labeled antibody that binds to the CD38 surface antigen. It is designed for use in flow cytometry applications.

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Lab products found in correlation

Clinimacs, by Miltenyi Biotec (3 mentions) Cd34 bv421, by BioLegend (3 mentions) Cd135 pe, by BioLegend (3 mentions) Cd45ra bv711, by BioLegend (3 mentions) Lineage cocktail bv510, by BD (3 mentions) Cd36 percpcy5, by BD (3 mentions) Cd110 apc, by BD (3 mentions) Cd41a apch7, by BD (3 mentions) Human insulin, by Merck Group (1 mentions) Human transferrin, by Merck Group (1 mentions) Iscove s modified dulbecco s medium, by Thermo Fisher Scientific (1 mentions)

4 protocols using cd38 pecf594

Isolation and Characterization of Hematopoietic Progenitor Cells

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Human granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood was CD34+ enriched (CliniMACS; Miltenyi), stained with Lineage cocktail-BV510 (BD), CD34-BV421 (Biolegend), CD38-PECF594(BD), CD45Ra-BV711, CD135-PE (Biolegend), CD36-PerCPCy5.5 (BD), CD110-APC (BD) and CD41a-APCH7(BD) antibodies. Human MEP (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38^midCD110⁺CD36⁻-CD41a⁻), ErP (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38^highCD110⁻), MkP (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38^midCD110⁺CD36⁻CD41a⁺) and CMP (Lin⁻CD34⁺CD45Ra⁻CD135⁺) were sorted on a FACSAria, as previously described (Sanada et al., 2016 (link)).

Xavier-Ferrucio J., Ricon L., Vieira K., Longhini A.L., Lazarini M., Bigarella C.L., Franchi G J.r., Krause D.S, & Saad S.T. (2017). Hematopoietic defects in response to reduced Arhgap21. Stem cell research, 26, 17-27.

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Isolation and Characterization of Hematopoietic Progenitor Cells

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Isolation of Hematopoietic Progenitor Subsets

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Human granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood was CD34⁺ enriched (CliniMACS; Miltenyi) and stained with Lineage cocktail-BV510 (BD Biosciences [BD]), CD34-BV421 (Biolegend), CD38⁻ PECF594 (BD), CD45Ra-BV711 (Biolegend), CD135-PE (Biolegend), CD36-PerCPCy5.5 (BD), CD110-APC (BD), and CD41a-APCH7 (BD) antibodies. Human MEPs (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38midCD110⁺CD36⁻CD41a⁻), MkPs (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38midCD110⁺CD36⁻CD41a⁺), and ErPs (Lin⁻CD34⁺CD45Ra⁻CD135⁻CD38hiCD110⁻) were sorted on a FACSAria, as previously described^{3 (link)}.

Scanlon V.M., Thompson E.N., Lawton B.R., Kochugaeva M., Ta K., Mayday M.Y., Xavier-Ferrucio J., Kang E., Eskow N.M., Lu Y.C., Kwon N., Laumas A., Cenci M., Lawrence K., Barden K., Larsuel S.T., Reed F.E., Peña-Carmona G., Ubbelohde A., Lee J.P., Boobalan S., Oppong Y., Anderson R., Maynard C., Sahirul K., Lajeune C., Ivathraya V., Addy T., Sanchez P., Holbrook C., Van Ho A.T., Duncan J.S., Blau H.M., Levchenko A, & Krause D.S. (2022). Multiparameter analysis of timelapse imaging reveals kinetics of megakaryocytic erythroid progenitor clonal expansion and differentiation. Scientific Reports, 12, 16218.

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Plasma Cell Differentiation of Memory B Cells

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The sorted gp140-specific memory B cells (2 × 10⁴/ml) from all LTNPs (10 R-ADCC, 3 NR-ADCC LTNPs) and total memory B cells from three HCs were cultured in a 96-well plate for 10 days at 37°C and 5% CO₂ in Iscove’s modified Dulbecco’s medium (Thermo Fisher) and 10% FBS along with human insulin (5 μg/ml) and human transferrin (50 μg/ml) (Sigma-Aldrich) and with different combinations of cytokines (detailed in Supplementary Table 1). On the 4th, 7th, and 10th day of culture, the supernatants were collected and stored at −20°C for further analysis. On the 10th day of culture, the cells were washed and stained for plasma cell markers: anti-human CD20 APC Cy7, CD38 PECF594, and CD138 PerCp Cy5·5 (BD Biosciences) and violet amine-reactive dye live-dead APC (Life Technologies) for 30 min at room temperature. The cells were acquired on BD FACSAria™ Fusion after fixing and analyzed by BD FlowJo (version 10.0) software. The gating strategy for the identification of plasma cells is depicted in Figure 2D.

Dhande J.R., Bagul R.D, & Thakar M.R. (2022). HIV-gp140-Specific Antibodies Generated From Indian Long-Term Non-Progressors Mediate Potent ADCC Activity and Effectively Lyse Reactivated HIV Reservoir. Frontiers in Immunology, 13, 844610.

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