Phosphorylated her2

(3-(4, 5-dimethyl-thiazol-2-yl)-2, 5-dipheny-ltetrazolium bromide (MTT), fluorescein isothiocyanate (FITC) and isoprophyl-β-D-thiogalactopranoside (IPTG) were purchased from Sigma Aldrench Chemical Inc. (St. Louis, MO, USA). All the antibodies including phosphorylated-HER2, -EGFR, -AKT, -extracellular regulated protein kinase (ERK), -p38 mitogen-activated protein kinase (MAPK), -c-Jun N-terminal kinase (JNK) monoclonal antibodies and anti-EGFR, -HER2, -AKT, -ERK, -p38MAPK, -JNK, -caspase 3, -caspase 7, -cleaved PARP antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-β-actin antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit/mouse antibodies were also purchased from by Cell Signaling Technology.

The whole-cell protein lysates or animal tumor tissue specimens were prepared as previously described [47 (link)] and analyzed by western blot with the following primary antibodies: phosphorylated STAT3 (p-STAT3, Tyr705), phosphorylated AKT (p-AKT, Ser473), phosphorylated MAPK (p-MAPK, Thr202/Tyr204), phosphorylated HER2 (p-HER2, Tyr1221/1222), phosphorylated EGFR (p-EGFR, Tyr1068), phosphorylated HER3 (p-HER3, Tyr1289), phosphorylated IGF-IR (p-IGF-IR, Tyr1316), STAT3, AKT, MAPK, EGFR, HER2, HER3, IGF-IR, PTEN, caspase 3 and 7 from Cell Signaling Technology (Denvers, MA, USA); FN, IL-6, IL-10, LIF, EGF, MUC1 (clone EP1024Y) and MUC4 (clone 8G-7) from Abcam (Cambridge, MA, USA).