Gta 120

Manufactured by Agilent Technologies

Sourced in Australia

The GTA-120 is a laboratory equipment product offered by Agilent Technologies. It is a device designed for specific core functions without further interpretation or extrapolation on its intended use.

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Lab products found in correlation

Vga 77, by Agilent Technologies (2 mentions) Spectra aa 240z, by Agilent Technologies (1 mentions) Aa280z, by Agilent Technologies (1 mentions) Aa280fs, by Agilent Technologies (1 mentions) Varian 240fs, by Agilent Technologies (1 mentions)

7 protocols using gta 120

Analytical Determination of Mercury and Cadmium

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Samples were analyzed using an Atomic absorption Spectrophotometer (Varian 240 FS, Mulgrave, Australia). THg was analyzed using the cold vapor generation accessory (Varian VGA-77) and Cd was analyzed with the support of a graphite tube atomizer (Varian GTA-120). Standard solutions were prepared from 1000 mg/L stock solutions (Fluka, Switzerland) with appropriate dilution. All of the instrumental parameters were set according to the manufacturer's recommended procedure.
Duplicate sample analysis and certified Quality Control Samples (CQM) were used to verify the analytical method. Canned fish offal, T/07243, and canned crab meat, T/07279QC from the Food Analysis Performance Assessment Scheme, FAPAS, Sand Hutton, York, UK were used, and the results are given in Table 1. The Limit of Quantification (LOQ) was 0.07 mg/kg and 0.006 mg/kg wet weight for THg and Cd, respectively. In the THg and Cd analysis, the precision is expressed as the relative standard deviation of three replicate samples and the value was maintained at less than 10%.Table 1

Obtained ±SD and certified concentrations (μg/kg, wet weight) in certified quality control materials (CQM).

Table 1

CQM		THg	Cd
T/07243	Certified	707 (469–946)	800 (535–1065)
T/07243	Obtained	722.55 ± 50.58	782.40 ± 70.41
T/07279	Certified	106 (59–152)	7.55 (5.76–9.33)∗
T/07279	Obtained	107.48 ± 6.45	7.40 ± 0.59∗

∗

mg/kg, SD – standard deviation.

Jinadasa B.K., Chathurika G.S., Jayasinghe G.D, & Jayaweera C.D. (2019). Mercury and cadmium distribution in yellowfin tuna (Thunnus albacares) from two fishing grounds in the Indian Ocean near Sri Lanka. Heliyon, 5(6), e01875.

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Trace Metal Analysis in Propolis

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Reference materials, reagent blanks, and propolis mineralized samples were analysed by Graphite Furnace Atomic Absorption Spectrometry (GFAAS) with Zeeman background correction (Spectra AA 240Z and GTA-120 Varian, Mulgrave, Australia) to determine Cd, Pb, Se, and As and Flame Atomic Absorption Spectrometry (FAAS) (Varian Model 55B, Palo Alto, USA) to determine Cu and K. The detection of metals in both equipment occurred according to the analytical parameters and temperature program indicated in the equipment manual for each metal analysed. The program temperature for each metal analysed by GFAAS is detailed in Table S1. Each sample was analysed in duplicate, and the spectrometer performed two readings of each sample, calibrator, and reference material. The average metal levels were measured in the reagent blank and subtracted from the metal content measured in the samples and reference material [25 (link)]. The resulting concentrations of each metal in the 19 propolis samples were expressed in micrograms (As, Cd, Pb, and Se) or milligrams (Cu and K) of metal per gram (dry weight), and then, the average metal concentration per sample was calculated.

Hodel K.V., Machado B.A., Santos N.R., Costa R.G., Menezes-Filho J.A, & Umsza-Guez M.A. (2020). Metal Content of Nutritional and Toxic Value in Different Types of Brazilian Propolis. The Scientific World Journal, 2020, 4395496.

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Hair Manganese Measurement Protocol

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Hair samples (

\sim 20 - 30

strands) were collected from the occipital region, within

2 mm

from the scalp, at one or two pregnancy visits (conducted at the same time as the pregnancy interviews and the urine sample collection). Samples were stored at 20–25°C (room temperature) and were shipped to the Federal University of Bahia, Brazil. The one-centimeter closest to the scalp from each hair sample was cleaned as described elsewhere (Menezes-Filho et al. 2009 (link)) and was analyzed for Mn using electrothermal atomic spectroscopy with Zeeman background correction (GTA-120; Varian, Inc.). Processed hair samples and reference materials were analyzed in duplicate and had CVs that ranged between 1.5 and 7.3%. Only two hair samples had Mn concentrations below the analytical LOD (

0.1 μ g / L

); their values were set at

LOD / \sqrt 2

Mora A.M., Córdoba L., Cano J.C., Hernandez-Bonilla D., Pardo L., Schnaas L., Smith D.R., Menezes-Filho J.A., Mergler D., Lindh C.H., Eskenazi B, & van Wendel de Joode B. (2018). Prenatal Mancozeb Exposure, Excess Manganese, and Neurodevelopment at 1 Year of Age in the Infants’ Environmental Health (ISA) Study. Environmental Health Perspectives, 126(5), 057007.

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Prenatal Manganese Exposure Assessment

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Hair samples (~ 20-30 strands) were collected from the occipital region, within 2 mm from the scalp, at enrollment (median = 19, interquartile range = 14-24 weeks gestation) and in the latter half of pregnancy (median = 31, interquartile range = 26-34 weeks gestation). Samples were stored in plastic bags at room temperature and shipped in batches to the Federal University of Bahia, Brazil. The one-centimeter closest to the scalp from each hair sample was cleaned as described elsewhere (Menezes-Filho et al. 2009 (link)) and analyzed for Mn using electrothermal atomic spectroscopy with Zeeman background correction (GTA-120, Varian Inc.). Mn was measured in 708 hair samples collected from 380 study participants; 328 women provided two hair samples during pregnancy and 52 provided only one (at enrollment). Only three hair samples had Mn concentrations below the limit of detection (LOD = 0.01 μg/g) and their values were set at LOD/2.

Mora A.M., van Wendel de Joode B., Mergler D., Córdoba L., Cano C., Quesada R., Smith D.R., Menezes-Filho J.A, & Eskenazi B. (2014). Maternal blood and hair manganese concentrations, fetal growth, and length of gestation in the ISA cohort in Costa Rica. Environmental research, 136, 47-56.

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Assessing Plant Fitness Traits and Zn Translocation

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At the peak of the growing season, after bait-lamina strips insertion in soil and along with soil sampling for Biolog^® ECO plates measurements, we scored a set of fitness traits as estimates of plant performance. These included: number of inflorescences, length of the tallest inflorescence (cm), height of the rosette (cm), and leaf area (cm²). Leaf area was analyzed using a nondestructive technique based on digital photographs analyzes with EASY LEAF AREA software (Easlon & Bloom, 2014 (link)). At the end of the experiment, plants from one experimental plot were harvested, their shoots ad roots washed in deionized water, and dried. To determine Zn concentration in shoots and roots ~0.3 g of the respective dry-ground plant tissue samples was mixed with 6 ml of HNO₃ (69%–70%) and HClO₄ (70%–72%) (4:1 v/v), left for 24 h, and boiled on a hot plate (Digestor 40 Auto, Foss Tecator, Sweden) at 282–284°C for 1.5–2 hr. Zinc concentration was determined using atomic absorption spectrometers (AAS; AA280FS, Varian; AA280Z, GTA 120, Varian, Australia) and the results ascertained using certified Standard Reference Material 1570a–spinach leaves (National Institute of Standards & Technology). The Zn translocation factor (TF_Zn) was calculated as the ratio between metal concentration in shoots and roots.

Hayakawa T., Satta Y., Gagneux P., Varki A, & Takahata N. (2001). Alu-mediated inactivation of the human CMP- N-acetylneuraminic acid hydroxylase gene. Proceedings of the National Academy of Sciences of the United States of America, 98(20), 11399-11404.

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Quantifying Manganese in Hair Samples

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Hair samples (~20–30 strands) were collected from the occipital region, cutting from 2 mm from the scalp, stored in plastic sampling bags at room temperature (20–25°C) and sent to the Federal University of Bahia Brazil for analysis (Mora et al., 2014 (link)). Briefly, the one centimeter of strands closest to the scalp were analyzed thought to represent last three-weeks’ excess Mn. Mn was measured using electrothermal atomic spectroscopy with Zeeman background correction (GTA-120; Varian, Inc. (Menezes-Filho et al., 2009 (link)). Samples below the L D (0.1 μg/g, n=2) were set at LOD/2.

Vargas A.C., Castañeda J.P., Liljedahl E.R., Mora A.M., Menezes-Filho J.A., Smith D.R., Mergler D., Reich B., Giffin A., Hoppin J.A., Lindh C.H, & van Wendel de Joode B. (2021). Exposure to common-use pesticides, manganese, lead, and thyroid function among pregnant women from the Infants’ Environmental Health (ISA) study, Costa Rica. The Science of the total environment, 810, 151288.

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Trace Metal Analysis in Food Samples

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Samples were grind using a domestic food blender and sieved, then 0.5 g of each sample mineralized in Teflon digestion vessels in a closed microwave accelerated system (CEM, MARS 6, USA) using 10 mL nitric acid (AR, Sigma, USA) as a reagent. Atomic absorption spectrophotometer (AAS) (Varian 240 FS, Varian Inc., Australia) was used to determine the mercury (Hg), cadmium (Cd), lead (Pb), arsenic (As), in the prepared samples. Vapour generation accessory (Varian VGA-77) with the closed-end cell was used for Hg determination. A graphite tube atomizer (Varian GTA-120) was used for Cd, As, and Pb. The calibration curves for all the metals were obtained with a series of standard solutions, which were prepared using the above mentioned standard solutions. All the samples were replicated thrice.

Jayasinghe G., Jinadasa B., & Sadaruwan N.A.G. (2022). Pathway of sodium alginate synthesis from marine brown algae, Sargassum wightii from Sri Lanka. Discover Food, 2(1).

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