We collected peritoneal effluents from EPS patients at the diagnosis of EPS and at the time of the peritoneal equilibrium test (PET) in control patients. The samples were collected after a 4 h dwell time and were frozen at a temperature of −80°C within 24 h. The peritoneal effluents were sent to a central laboratory for MMP-2 measurements using the Human MMP-2 ELISA kit (Invitrogen: #KHC3081) according to the manufacturer's protocol. Effluent MMP-2 levels (μg/mg) were standardized for per mg/dl of total protein in the effluent. None of the dialysate MMP-2 levels was below the detection limit. There were no missing data.
Human mmp 2 elisa kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Human MMP-2 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human matrix metalloproteinase-2 (MMP-2) levels in biological samples. The kit utilizes a specific antibody coated on a microplate to capture MMP-2 from the sample, which is then detected using a horseradish peroxidase (HRP)-conjugated detection antibody and a colorimetric substrate.
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Lab products found in correlation
3 protocols using human mmp 2 elisa kit
1
Measuring Peritoneal MMP-2 in EPS
2
MMP-2 Detection in Culture Supernatants
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Culture supernatants were collected, neither concentrated nor diluted, and assayed for MMP-2 detection by using a Human MMP-2 ELISA Kit (Invitrogen, Camarillo, CA), following manufacturer's instructions.
3
Quantifying MMP-2 and MMP-9 Levels
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The concentrations of MMP-2 and MMP-9 were further evaluated in both cell lines. Cell culture supernatants were collected at 48 h after transfection. MMP-2 and MMP-9 concentrations were measured using human MMP-2 ELISA Kit and human MMP-9 ELISA Kit (Invitrogen Corporation, Carlsbad, United States) according to the manufacturer’s instructions. The optical density (OD) value at 450 nm was record using a microplate reader.
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