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Mouse ige elisa kit

Manufactured by Thermo Fisher Scientific
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The Mouse IgE ELISA kit is a quantitative immunoassay used to measure IgE levels in mouse serum or plasma samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify mouse IgE.

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22 protocols using mouse ige elisa kit

1

Quantification of Serum Allergic Markers

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An ELISA for total serum IgE was performed according to the manufacturer’s instructions using a Mouse IgE ELISA kit (#88-50460-88; Invitrogen); serum was applied at a concentration of 1:50. To measure peanut-specific IgE, a clear flat-bottom immuno nonsterile 96-well plate (#442404; Thermo Fisher Scientific) was coated with peanut extract in PBS (10 µg/ml) instead of the anti-IgE capture antibody, and the rest of the assay was performed using Mouse IgE ELISA kit (#88-50460-88; Invitrogen) according to the manufacturer’s instructions; serum was applied at a concentration of 1:50. To measure peanut-specific IgG1, a clear flat-bottom immuno nonsterile 96-well plate (#442404; Thermo Fisher Scientific) was coated with peanut extract in PBS (10 µg/ml) instead of the anti-IgG1 capture antibody, the rest of the assay was performed using a Mouse IgG1 ELISA kit (#88-50410-88; Invitrogen), according to the manufacturer’s instructions; serum was applied at 1:5,000. Serum MCPT-1 levels were measured using an MCPT-1 (mMCP-1) Mouse ELISA Kit (#88-7503-88; Invitrogen) according to the manufacturer’s instructions; serum was applied at a concentration of 1:50.
Tauber M., Basso L., Martin J., Bostan L., Pinto M.M., Thierry G.R., Houmadi R., Serhan N., Loste A., Blériot C., Kamphuis J.B., Grujic M., Kjellén L., Pejler G., Paul C., Dong X., Galli S.J., Reber L.L., Ginhoux F., Bajenoff M., Gentek R, & Gaudenzio N. (2023). Landscape of mast cell populations across organs in mice and humans. The Journal of Experimental Medicine, 220(10), e20230570.
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2

Quantifying Serum Immune Markers in Mice

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An ELISA for total serum IgE was performed according to the manufacturer’s instructions using a Mouse IgE ELISA kit (#88-50460-88; Invitrogen); serum was applied at a concentration of 1:50. To measure peanut-specific IgE, a clear flat-bottom immuno nonsterile 96-well plate (#442404; Thermo Fisher Scientific) was coated with peanut extract in PBS (10 µg/ml) instead of the anti-IgE capture antibody, the rest of the assay was performed using Mouse IgE ELISA kit (#88-50460-88; Invitrogen) according to the manufacturer’s instructions; serum was applied at a concentration of 1:50. To measure peanut-specific IgG1, a clear flat-bottom immuno nonsterile 96-well plate (#442404; Thermo Fisher Scientific) was coated with peanut extract in PBS (10 µg/ml) instead of the anti-IgG1 capture antibody and the rest of the assay was performed using a Mouse IgG1 ELISA kit (#88-50410-88; Invitrogen), according to the manufacturer’s instructions; serum was applied at 1:5,000. Serum MCPT-1 levels were measured using an MCPT-1 (mMCP-1) Mouse ELISA Kit (#88-7503-88; Invitrogen) according to the manufacturer’s instructions; serum was applied at a concentration of 1:50.
Tauber M., Basso L., Martin J., Bostan L., Pinto M.M., Thierry G.R., Houmadi R., Serhan N., Loste A., Blériot C., Kamphuis J.B., Grujic M., Kjellén L., Pejler G., Paul C., Dong X., Galli S.J., Reber L.L., Ginhoux F., Bajenoff M., Gentek R, & Gaudenzio N. (2023). Landscape of mast cell populations across organs in mice and humans. The Journal of Experimental Medicine, 220(10), e20230570.
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3

Measuring Serum IgE Levels by ELISA

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The serum IgE concentrations were determined using a mouse IgE ELISA kit (eBioscience, San Diego, CA, USA) at 450 nm. The difference between the sample absorbance and the mean of the negative control absorbance was expressed as the result.
Lee J., Ree J., Kim H.J., Kim H.J., Kim W.J., Choi T.G., Lee S., Hong Y.K., Hong S.B, & Park Y.I. (2022). Anti-Apoptotic and Anti-Inflammatory Effects of an Ethanolic Extract of Lycium chinense Root against Particulate Matter 10-Induced Cell Death and Inflammation in RBL-2H3 Basophil Cells and BALB/c Mice. Plants, 11(19), 2485.
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4

IgE Quantification in Mouse Sera

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Serum samples were obtained from blood taken during exsanguination of the mice after completing the sensitization, and stored at −80°C until use. Total IgE levels in sera were detected using the Mouse IgE ELISA kit (eBioscience, San Diego, CA, USA) in duplicate. The optical density was measured at 450 nm.
Kim H.J., Lee S.H, & Hong S.J. (2019). Antibiotics-Induced Dysbiosis of Intestinal Microbiota Aggravates Atopic Dermatitis in Mice by Altered Short-Chain Fatty Acids. Allergy, Asthma & Immunology Research, 12(1), 137-148.
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5

Evaluating Immune Responses in Mice

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Both left lung and spleen were removed. The measurement of the immune response was performed using the supernatant of the homogenised lung and spleen. These organs were analysed for IL-5, IL-13, IL-17E (IL-25) and IL-33 cytokines by ELISA DuoSet from R&D Systems (Minneapolis, MN, USA) following the manufacturer’s instructions.
Blood was collected by cardiac puncture and the total serum IgE was measured using the mouse IgE ELISA Kit (Invitrogen), according to the manufacturer’s instructions.
Vélez-del-Burgo A., Sánchez P., Suñen E., Martínez J, & Postigo I. (2021). Purified Native and Recombinant Major Alternaria alternata Allergen (Alt a 1) Induces Allergic Asthma in the Murine Model. Journal of Fungi, 7(11), 896.
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6

Measurement of Serum IgE Levels

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Serum total IgE concentration was measured using mouse IgE ELISA kit (Invitrogen, catalog 88–50460) as previously described.56
Yuan H., Chen J., Hu S., Oriss T.B., Kale S.L., Das S., Nouraie S.M., Ray P, & Ray A. (2021). Early life exposure to house dust mite allergen prevents experimental allergic asthma requiring mitochondrial H2O2. Mucosal immunology, 15(1), 154-164.
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7

Measuring Serum IgE in Mice

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Blood collected from mice was placed in an e-tube and centrifuged at 4 °C and 850× g for 30 min. Following centrifugation, supernatant was stored at −70 °C until measurement. Serum IgE was measured using an enzyme-linked immunosorbent assay (ELISA). Each sample was added onto a 96-well plate treated with the primary antibody using a mouse IgE ELISA Kit (Invitrogen, Waltham, MA, USA), and washed four times with the working solution. After washing, the samples were treated with the secondary antibody, HRP-conjugated goat anti-mouse IgE (1:10,000), for 1 h after which a color development reagent was used. Following color development, the reaction was stopped using a stop solution, and absorbance was measured at 450 nm using a microplate reader (Spectra Max 250, Molecular Devices, Sunnyvale, CA, USA).
Kim S.Y., Yoon T.H., Na J., Yi S.J., Jin Y., Kim M., Oh T.H, & Chung T.W. (2022). Mesenchymal Stem Cells and Extracellular Vesicles Derived from Canine Adipose Tissue Ameliorates Inflammation, Skin Barrier Function and Pruritus by Reducing JAK/STAT Signaling in Atopic Dermatitis. International Journal of Molecular Sciences, 23(9), 4868.
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8

Ovalbumin IgE and IgG1 Antibody Levels

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Total and OVA-specific serum IgE/IgG1 Ab levels at different time-points during the allergen immunization protocol were measured using Mouse IgE ELISA kit (Invitrogen), Mouse anti-OVA IgE assay kit (Chondrex), and anti-Ovalbumin IgG1 ELISA kit (Cayman Chemical), correspondingly. All ELISA plates were read on either iMark (Bio-Rad) or SpectroStar Nano (BMG Labtech) microplate reader using the manufacturer specified wavelengths for each assay.
Chapoval S.P., Gao H., Fanaroff R, & Keegan A.D. (2024). Plexin B1 controls Treg numbers, limits allergic airway inflammation, and regulates mucins. Frontiers in Immunology, 14, 1297354.
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9

Measurement of Serum IgE Levels

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Serum total IgE concentration was measured using mouse IgE ELISA kit (Invitrogen, catalog 88–50460) as previously described.56
Yuan H., Chen J., Hu S., Oriss T.B., Kale S.L., Das S., Nouraie S.M., Ray P, & Ray A. (2021). Early life exposure to house dust mite allergen prevents experimental allergic asthma requiring mitochondrial H2O2. Mucosal immunology, 15(1), 154-164.
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10

Comprehensive Cytokine Profiling in Murine Models

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We used the Th1/Th2 U-PLEX (K15069L-2; Meso Scale Discovery [MSD]) platform to analyze cytokines in protein extract and BAL supernatant. This platform uses a 10-spot U-PLEX plate and unique linkers to analyze samples. Biotinylated capture Abs are assigned to linkers, which correspond to unique spots in the U-PLEX plate. The analytes tested were IFN-γ, IL-1β, IL-2, IL-4, IL-5, KC, IL-10, IL-12p70, TNF-α, and IL-13. The MSD instrument applies a voltage to the plate electrodes, causing the capture labels to emit light. The intensity of the emitted light is measured. The BAL supernatant was at 1:1 dilution, and the protein extraction was at 1:2 dilution. Dilutions were used in the assay and then corrected for in the analysis. Blood was collected via cardiac puncture and stored in K2EDTA tubes. The tubeswere centrifuged at 500 × g for 10 min, and the plasma was collected. We used the Mouse IgE ELISA Kit (EMIGHE; Thermo Fisher Scientific) to analyze for IgE. The plasma was diluted 10,000-fold as suggested by the vendor and corrected for in the analysis. The lung protein was extracted from homogenized lung tissue. We used the IL-17 ELISA Kit (GR3332177–1; Abcam) to analyze for IL-17 cytokine. The lung protein extract was diluted 1:1. The dilution was corrected for in the analysis.
Weiss K., Wanner N., Queisser K., Frimel M., Nunn T., Myshrall T., Sangwan N., Erzurum S, & Asosingh K. (2021). Barrier Housing and Gender Effects on Allergic Airway Disease in a Murine House Dust Mite Model. ImmunoHorizons, 5(1), 33-47.
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