Genomic dna screentape 5067 5366

Where possible, up to 10 mL of peripheral blood was collected into Cell-Free DNA Collection Tubes (Streck, La Vista). Samples were centrifuged twice for 10 min, first at 1600 g and at up to 16,000 g to remove cellular contents and/or debris. Samples were stored at −80°C until cfDNA extraction. Local protocols to isolate plasma and CSF were used for the remaining liquid biopsies cases, collected from different sources. cfDNA isolation from plasma and CSF supernatant was performed using the QIAamp circulating nucleic acid kit (Qiagen, 55114) following quantification using the Qubit fluorometer (ThermoFisher Scientific, dsDNA HS Assay kit, Q32854) and fragment analysis by 2200 and 4200 TapeStation (Agilent, Genomic DNA ScreenTape 5067–5366).

Up to 10 mL of peripheral blood was collected into Cell-Free DNA Collection Tubes (Streck). Samples were centrifuged twice for 10 minutes—first at 1,600 × g and at up to 16,000 × g to remove cellular contents and/or debris. Cell-free DNA isolation from plasma was performed using the QIAamp circulating nucleic acid kit (Qiagen, 55114) following quantification using the Qubit fluorometer (Thermo Fisher Scientific, dsDNA HS Assay kit, Q32854) and fragment analysis by 2200 and 4200 TapeStation (Agilent, Genomic DNA ScreenTape 5067–5366).