The resulting cell suspension was then separated at 300 × g for 5 min at 4°C and resuspended in 90 mL of MBB along with 10 mL of anti-CD45 magnetic beads-conjugated antibody (Miltenyi) for each 10ˆ7 cell sample. The cell suspension was incubated for 15 min at 4°C, then diluted with 2 mL of MBB and centrifuged. The resulting cell pellet was resuspended in 500 mL of MBB, applied onto hydrated MS-columns (Miltenyi), washed three times with 500 mL of MBB, and collected with 1 mL of MBB through piston elution.
Anti cd45 magnetic beads conjugated antibody
Anti-CD45 magnetic beads-conjugated antibody is a laboratory reagent used for the isolation and enrichment of CD45-positive cells from complex biological samples. The antibody is coupled to magnetic beads, allowing for efficient separation of the target cells using a magnetic field.
2 protocols using anti cd45 magnetic beads conjugated antibody
CD45+ Cell Isolation from Samples
The resulting cell suspension was then separated at 300 × g for 5 min at 4°C and resuspended in 90 mL of MBB along with 10 mL of anti-CD45 magnetic beads-conjugated antibody (Miltenyi) for each 10ˆ7 cell sample. The cell suspension was incubated for 15 min at 4°C, then diluted with 2 mL of MBB and centrifuged. The resulting cell pellet was resuspended in 500 mL of MBB, applied onto hydrated MS-columns (Miltenyi), washed three times with 500 mL of MBB, and collected with 1 mL of MBB through piston elution.
Isolation of Stromal Vascular Cells and Mitochondria
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