Polyethylene glycol 1450 solution peg 1450

All the strains used in this study were from Fujian Agriculture and Forestry University (Fujian, China). The anti-His 6 tag mAb, anti-GST tag mAb and horseradish peroxidase (HRP) labeled goat anti-mouse IgG were purchased from ZSGB-Bio Co., Ltd (Beijing, China). D2-40 antibody and all paraffin-fixed tissue sections were supported by Fuzhou Maixin Biotech Co., Ltd (Fuzhou, China). Hypersensitivity ECL chemiluminescence detection kit was purchased from Wuhan Sanying Co., Ltd (Wuhan, China). Hypoxanthine, aminopterin and thymidine supplement (HAT), hypoxanthine and thymidine supplement (HT) and polyethylene glycol 1450 solution (PEG 1450) were purchased from Sigma. RPMI medium 1640 powder and fetal bovine serum (FBS) were purchased from Gibco.

The anti-6 × His tag monoclonal antibody and HRP-labeled goat anti-mouse IgG were purchased from ZSGB-Bio Co., Ltd. (Beijing, China). The cocktail of LK2H10 and PHE5 antibodies, and MaxVisionTM/HRP IHC kit were provided by Fuzhou Maixin Biotech Co., Ltd. (Fuzhou, China). SP12 antibody was from Thermo Fisher Scientific (Shanghai, China). Hypersensitivity ECL chemiluminescence detection kit was purchased from Wuhan Sanying Co., Ltd. (Wuhan, China). Myeloma cells SP2/0 were stored in our laboratory. Hypoxanthine, aminopterin and thymidine supplement (HAT), hypoxanthine and thymidine supplement (HT) and polyethylene glycol 1450 solution (PEG 1450) were purchased from Sigma-Aldrich (Shanghai, China). RPMI medium 1640 powder and fetal bovine serum (FBS) were purchased from Thermo Fisher (Shanghai, China). Balb/c mice were from Wushi Animal Laboratory (Shanghai, China). QuickAntibody-Mouse5W adjuvant (Quickadjuvant) was purchased from Beijing Biodragon Immunotechnologies Co., Ltd. (Beijing, China). All animal experiments obeyed the protocols approved by the Animal Ethics Committee of the Fujian Agriculture and Forestry University.