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Cocktail of proteases inhibitors

Manufactured by Merck Group
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Sourced in United States

The Cocktail of proteases inhibitors is a laboratory product that contains a mixture of compounds that inhibit the activity of proteases, which are enzymes that break down proteins. This product is commonly used in research settings to preserve the integrity of protein samples during extraction, purification, and analysis.

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Lab products found in correlation

Bicinchoninic acid kit, by Thermo Fisher Scientific (1 mentions) Ripa buffer, by Merck Group (1 mentions)

Close spelling variants of this product

Cocktail of protease inhibitors Inhibitor of proteases cocktail Cocktails of protease inhibitors and phosphatase inhibitors Cocktails of Protease inhibitors Cocktails of protease and phosphatase inhibitors Proteases inhibitor cocktail Protease inhibitor cocktail Proteases Inhibitors Cocktail Protease inhibitors cocktail Protease inhibitors

2 protocols using cocktail of proteases inhibitors

1

Quantifying Alzheimer's Peptide in Flies

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Five fly heads from our line GMR/Aβ42 carrying two copies of the transgene and 5 heads from the GMR/Hj2.12 line were homogenized and proteins extracted in 50 mM Tris–HCl, pH 8 containing 5 M guanidine and a cocktail of proteases inhibitors (Sigma St. Louis, MO). After centrifugation at 10,000 × g, proteins in the supernatant were quantified using a bicinchoninic acid kit (Thermo Scientific, Rockford, IL). Aβ42 concentration was determined using a commercial capture ELISA kit (Invitrogen Frederick, MD) following the manufacturer’s instructions.
Marcora M.S., Fernández-Gamba A.C., Avendaño L.A., Rotondaro C., Podhajcer O.L., Vidal R., Morelli L., Ceriani M.F, & Castaño E.M. (2014). Amyloid peptides ABri and ADan show differential neurotoxicity in transgenic Drosophila models of familial British and Danish dementia. Molecular Neurodegeneration, 9, 5.
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2

Spinal Cord Injury: Neurotrophic Factor Analysis

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Five animals in the CytoCon group were sacrificed at 3 days after SCI, then five animals each from the CytohUCB group and CytoCon group were sacrificed at 7, 14, 21 and 28 days after SCI. Following sacrifice, the spinal cord (T7–T12) was extracted and homogenized with a cocktail of proteases inhibitors (Sigma, USA) in RIPA buffer (Millipore, USA), then centrifuged at 10,000 × g and 4℃. Supernatants were harvested and quantitative analysis of nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) was conducted using an ELISA kit (Boster Biological Technology, China).
Chung H.J., Chung W.H., Lee J.H., Chung D.J., Yang W.J., Lee A.J., Choi C.B., Chang H.S., Kim D.H., Suh H.J., Lee D.H., Hwang S.H., Do S.H, & Kim H.Y. (2016). Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats. Journal of Veterinary Science, 17(1), 97-102.
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