Bio-Rad Bio-Plex Luminex 200 multiplex assay system (Bio-Rad) was used to determine the levels of specific proteins of the insulin-like growth factor 1 (IGF-1)/Akt pathway (IGF-1, p70S6K, and p-p70S6K), with actin as a reference. For the analysis, 10 µg of skeletal muscle lysate protein was used. The analysis procedure was performed according to 'manufacturer's recommendations. Data were collected and processed using the Bio-Plex Manager software (Bio-Rad). The results were normalized to the amount of actin in the samples.
Bio plex luminex 200 multiplex assay system
Manufactured by Bio-Rad
The Bio-Plex Luminex 200 multiplex assay system is a laboratory instrument designed for the simultaneous detection and quantification of multiple analytes in a single sample. The system utilizes flow cytometry technology and magnetic microspheres coated with specific capture antibodies to allow for the rapid and efficient analysis of a wide range of biomolecules, including proteins, cytokines, and nucleic acids.
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Lab products found in correlation
2 protocols using bio plex luminex 200 multiplex assay system
1
Multiplex Analysis of IGF-1/Akt Pathway
2
Murine and Human Serum Cytokine Analysis
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Murine blood samples were collected using the submandibular bleeding method and serum samples were subsequently isolated. Human and murine serum samples were immediately stored at ≤ −20 °C for later use. In order to analyze cytokine (human and murine IL-6, murine IL-23A, and IL-17A) serum levels, the MILLIPLEX® MAP Kit (Merck Millipore) using Bio-Plex® Luminex 200 multiplex assay system (Bio-Rad) was employed according to the manufacturer’s protocol. Human serum levels of sIL-6 and sIL-6Rα were measured using Enzyme-Linked Immuno Sorbent Assay (ELISA) kit (Duoset and Quantikine; R&D systems) according to the manufacturer’s protocol.
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