5 5 6 6 tetrachloro 1 1 3 3 tetraethylbenzimi dazolylcarbocyanine iodide jc 1

Manufactured by Merck Group

Sourced in United States

5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimi-dazolylcarbocyanine iodide (JC-1) is a fluorescent dye used to assess mitochondrial membrane potential in cells. It exhibits potential-dependent accumulation in mitochondria, indicated by a shift in fluorescence emission from green (monomeric form) to red (J-aggregate form).

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Lab products found in correlation

Ham s f 12k medium, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Tio2 np, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) Fetal bovine serum (fbs), by American Type Culture Collection (1 mentions) Horse serum, by Harvard Bioscience (1 mentions) Propidium iodide stain, by Merck Group (1 mentions) Hanks balanced salt solution (hbss), by Lonza (1 mentions) Fetal bovine serum (fbs), by Harvard Bioscience (1 mentions) 2 deoxy 3h d glucose, by American Radiolabeled Chemicals (1 mentions) 14c palmitate, by American Radiolabeled Chemicals (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Mouse anti bcl 2 sc 7382, by Santa Cruz Biotechnology (1 mentions) Mouse anti β actin sc 47778, by Santa Cruz Biotechnology (1 mentions) Goat anti mouse igg hrp sc 2031, by Santa Cruz Biotechnology (1 mentions)

3 protocols using 5 5 6 6 tetrachloro 1 1 3 3 tetraethylbenzimi dazolylcarbocyanine iodide jc 1

Nanoparticle-Induced Mitochondrial Dysfunction

Cited 1 time

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Fetal bovine serum (FBS) was purchased from American Type Culture Collection (ATCC) (Manassas, VA). Ham’s F-12K medium with added L-glutamine was purchased from Fisher Scientific (Pittsburgh, PA). Trypsin–EDTA (ethylenediaminetetraacetic acid) (0.25%), and 0.1 M phosphate-buffered saline (PBS) were purchased from Life Technology Co. (Carlsbad, CA). Ultra-pure water was generated with a Milli-Q system (Millipore, Bedford, MA). The cetyltrimethylammonium bromide (CTAB), ethyltriethoxysilane (ETEOS), (3-glycidoxypropyl) trimethoxysilane (GPTMS), mitochondria inner membrane potential depolarization reagent CCCP, TiO₂ NPs at 99.0% purity, pH-sensitive dye HPTS, and mitochondrial potential indicator dye 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimi-dazolylcarbocyanine iodide (JC-1) (Perelman et al., 2012 (link)) were purchased from Sigma-Aldrich (Saint Louis, MO).

Yang Q., Zhang X., Song Y., Li K., Shi H., Xiao H, & Ma Y. (2020). Label-Free in Situ pH Monitoring in a Single Living Cell Using an Optical Nanoprobe. Medical devices & sensors, 3(3), e10079.

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Aspalathin and PPAG Cardiomyoblast Analysis

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Aspalathin (ca. 98%, batch SZI-356-54) and PPAG (99% purity; batch: MC1(2)-248–91D) were obtained from High Force Research (Durham, UK). Embryonic ventricular rat heart derived H9c2 cardiomyoblasts (CRL-1446) were from American Type Culture Collection (Manassas, VA, USA); 14C-palmitate and 2-deoxy-[³H]-D-glucose were from American Radiolabeled Chemicals (St. Louis, MI, USA); fetal bovine serum (FBS) and horse serum were from Biochrom (Berlin, Germany); Dulbecco’s Modified Eagle’s Medium (DMEM), Dulbecco’s Phosphate Buffered Saline (DPBS), and Hank’s Balanced Salt Solution (HBSS) were from Lonza (Basel, Switzerland); 2′, 7′-dichlorofluorescein diacetate (DCFH-DA) fluorescent dye was from Cell Biolabs Inc. (San Diego, CA, USA); and 7-amino-4chloromethylcoumarin (CellTracker Blue CMAC) and TRIzol reagent were from Invitrogen Corp. (Carlsbad, CA, USA). All other chemicals, including 5,5′6,6′-tetrachloro-1,1′,3,3-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) and propidium iodide stain were obtained from Sigma-Aldrich (St. Louis, MI, USA).

Dludla P.V., Muller C.J., Louw J., Mazibuko-Mbeje S.E., Tiano L., Silvestri S., Orlando P., Marcheggiani F., Cirilli I., Chellan N., Ghoor S., Nkambule B.B., Essop M.F., Huisamen B, & Johnson R. (2020). The Combination Effect of Aspalathin and Phenylpyruvic Acid-2-O-β-d-glucoside from Rooibos against Hyperglycemia-Induced Cardiac Damage: An In Vitro Study. Nutrients, 12(4), 1151.

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Cytotoxicity and Apoptosis Assay in Cells

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High glucose Dulbecco's modified Eagle's medium (DMEM), Roswell Park Memorial Institute 1640 medium (RPMI), fetal bovine serum (FBS), penicillin-streptomycin were obtained from Bioidea (Iran). Nisin, 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide (MTT), Dimethyl sulfoxide (DMSO) and 5,5',6,6'-tetrachloro-1,1',3,3'tetraethylbenzimidazolylcarbocyanine iodide (JC-1) were provided from Sigma-Aldrich (USA).
Mouse anti-Bcl-2 (sc-7382), mouse anti-β-actin (sc-47778), mouse anti-Bax (sc-20067), and goat anti-mouse IgG-HRP (sc-2031) were provided from Santa Cruz Biotechnology (USA). All other reagents were purchased from Sigma Aldrich (USA) and were of analytical grade. D r a f t 5

Sadri H., Aghaei M, & Akbari V. (2022). Nisin induces apoptosis in cervical cancer cells via reactive oxygen species generation and mitochondrial membrane potential changes. Biochemistry and cell biology = Biochimie et biologie cellulaire, 100(2).

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