Fitc conjugated anti mouse h 2kb antibody

OVA⁺ EO771 and NY-ESO-1⁺ MDA-MB-468 cells were seeded at 5 × 10³ cells/well in the 8-well slide (Millicell EZ slide, R8AA09916) and treated with 1 μM drug (BML-210, CUDC-101 or GSK-LSD1) for 48 h. FITC-conjugated anti-mouse H-2Kb antibody (Biolegend, dilution 1:50) and FITC-conjugated anti-HLA-A2 (Biolegend, dilution 1:50) were used to stain OVA⁺ EO771 and NY-ESO-1⁺ MDA-MB-468 cells, respectively, for 2 h. For cell nucleus staining, cells were incubated with 0.5% Triton X-100/PBS for 10 min and stained with DAPI (Sigma-Aldrich, dilution 1:100) for 15 min. The slides were rinsed in PBS twice and mounted with ProLong Gold anti-fade mountant (Invitrogen, P36930). Fluorescence images were captured using Leica TCS SP8 (upright high-speed multiphoton) confocal imaging system. 3-D images were analyzed by the Imaris x64 8.1.2 software.