Secondary goat antirabbit irdye 680 conjugated antibody

Rabbit polyclonal antibody against phospho-PDGFRβ (catalog n. sc-21902) and goat polyclonal antibody against PDGFRβ (catalog n. sc-1627) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Rabbit monoclonal antibody (EP1255Y) against MMP9, rabbit polyclonal antibody against NG2 (catalog num. ab129051), rabbit monoclonal antibody (E184) against α smooth muscle Actin (α-SMA, catalog n. ab32575), rabbit polyclonal antibody against transforming growth factor beta-1 proprotein (TGF-β1, catalog n. ab66043), and rabbit monoclonal (EPR3776) against vimentin (catalog n. ab92547) were purchased from Abcam (Cambridge, UK). Rabbit polyclonal antibody against fibroblast-specific protein 1 (S100A4) (catalog n. ABF32) was purchased from Millipore (Darmstadt, Germany). Mouse monoclonal antibody (BA3R) against β–actin (catalog n. MA5-15739) was purchased from Invitrogen, Thermo Fisher Scientific (Waltham, MA, USA). Secondary goat antirabbit IRDye 680 conjugated antibody (catalog n. 926-32221) and secondary goat antimouse IRDye 800 conjugated antibody (926-32210) were purchased from LI-COR (Lincoln, NE, USA). Imatinib (Glivec) was obtained from Novartis (Basel, Switzerland). Media, antibiotics, and other reagents for cell culture were obtained from Invitrogen, Thermo Fisher Scientific.

Mouse monoclonal antibody against cPLA2 (catalog n. sc-454) and mouse monoclonal p44/42 MAPK (Erk1/2, catalog sc-135900) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit polyclonal antibody against phospho-cPLA2 (catalog n. 2831S), phospho-p44/42 MAPK (phospho-Erk1/2, catalog n. 9101) were purchased from Cell Signaling Technology (Danvers, MA, USA); rabbit polyclonal antibody against iPLA2 (catalog n. ab23706) and mouse monoclonal antibody against β-actin (catalog n. ab8226) were purchased from Abcam (Cambridge, UK). Secondary goat anti-rabbit IRDye 680 conjugated antibody (catalog n. 926-32221) and secondary goat anti-mouse IRDye 800 conjugated antibody (catalog n. 926-32210) were purchased from LI-COR Biosciences, (Lincoln, NE, USA). VEGF-A was from Peprotech (Rocky Hill, NJ, USA). Arachidonoyl trifluoro-methyl ketone (AACOCF3), a cPLA2 inhibitor, and iPLA2 inhibitor, bromoenol lactone (BEL) were purchased from Calbiochem (La Jolla, CA, USA). Aflibercept (Eylea^®) was from Bayer Pharma (Berlin, Germany). Media, antibiotics, and other reagents for cell cultures were from Invitrogen Thermo Fisher Scientific (Monza, Italy).

VEGF-A was acquired from Peprotech (Rocky Hill, NJ, USA); PGE2 came from Cayman Chemical (Ann Arbor, MI, USA); mouse monoclonal antibodies against cPLA₂ were purchased from Santa Cruz (Santa Cruz, CA, USA); mouse monoclonal antibody against GAPDH was purchased from Abcam (Cambridge, UK); rabbit polyclonal antibodies against phospho-cPLA₂ and COX-2 were purchased from Cell Signaling Technology (Danvers, MA, USA); secondary goat anti-mouse TEXAS RED conjugated antibody was purchased from Calbiochem; and secondary goat anti-rabbit IRDye 680 conjugated antibody was purchased from LI-COR (Lincoln, Dearborn, MI, USA). Cisplatin and dexamethasone were purchased from Sigma-Aldrich (Munich, Germany). Media, antibiotics and other reagents for cell cultures came from Innoprot (Elexalde Derio, Spain).