Y 1196

CP-724714 HER2 inhibitor was purchased from Selleckchem (Houston, TX, USA). Lapatinib and isotype control human IgG were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trastuzumab was purchased from Roche (Basel, Switzerland). Mouse monoclonal anti-human HER2 (9G6) and (A-2), anti-human EGFR (A-10), and anti-human HER3 (RTJ.2) antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Rabbit polyclonal anti-human phospho-HER2 Y-1005, Y-1112, Y-1127, Y-1139, Y-1196, and Y-1248 antibodies were purchased from FroggaBio (Toronto, ON, Canada). All other chemicals were purchased from Sigma-Aldrich.

HER2 inhibitor CP-724714 was from Selleckchem (Houston, TX, USA). Normal human IgG were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trastuzumab was from Roche (Basel, Switzerland). Mouse monoclonal antibodies to human EGFR (1005), HER2 (A-2) (sc-393712), human EGFR (1005), Akt1/2 (H-136), Erk1/2 (k-23), pT202/Y204 Erk1/2 were from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Rabbit monoclonal antibodies to phospho-Akt (Ser473) and phosphor(Thr308), and rabbit monoclonal antibody to HER2/ErbB2 (29D8) were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit polyclonal antibodies to phospho-HER2 Y-1005, Y-1112, Y-1127, Y-1139, Y-1196, and Y-1248 were from FroggaBio (Toronto, ON, Canada). Secondary antibodies used for Western analysis, including Anti-rabbit and anti-mouse RDye^® 800 CW and RDye^® 650, were from LI-COR biotechnology Inc. (Lincoln, NE, USA). Invitrogen™ Cholera Toxin Subunit B (Recombinant) and Alexa Fluor™ 488 Conjugate were purchased from Fisher Scientific (Ottawa, ON, Canada). All other chemicals were purchased from Sigma-Aldrich (Oakville ON, Canada).