Human colorectal carcinoma cell lines DLD1 and LoVo were obtained from the ATCC (USA). DLD1 cells were cultured in RPMI 1640 GlutaMax medium (Gibco, USA) supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin (Biosera, France). LoVo cells were cultured in F-12K medium (Bioconcept, Switzerland) supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin (Biosera, France). Cells were cultivated at 37°C in a HF90 humidified incubator (Heal Force, China) containing 5% CO2. Experiments were performed three times.
Hf90 humidified incubator
Manufactured by Heal Force
Sourced in China
The HF90 humidified incubator is a laboratory equipment designed to provide a controlled environment for cell and tissue culture applications. The core function of this device is to maintain a consistent temperature and humidity level within the incubation chamber, creating an optimal environment for the growth and development of biological samples.
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Lab products found in correlation
2 protocols using hf90 humidified incubator
1
Cultivation and Maintenance of Colorectal Cancer Cell Lines
2
Cultivation and Maintenance of Colorectal Cancer Cell Lines
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Human colorectal carcinoma cell lines DLD1 and LoVo were obtained from the ATCC (USA). DLD1 cells were cultured in RPMI 1640 GlutaMax medium (Gibco, USA) supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin (Biosera, France). LoVo cells were cultured in F-12K medium (Bioconcept, Switzerland) supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin (Biosera, France). Cells were cultivated at 37°C in a HF90 humidified incubator (Heal Force, China) containing 5% CO2. Experiments were performed three times.
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