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Glutamine gln

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Glutamine (Gln) is an amino acid that serves as a core building block for proteins. It is a naturally occurring substance and an important component in various cellular processes.

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Lab products found in correlation

Minimal essential medium, by Thermo Fisher Scientific (3 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (3 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (3 mentions) Hek293 cell, by Thermo Fisher Scientific (2 mentions) Vero cells, by Thermo Fisher Scientific (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Cocl2 6h2o, by Merck Group (1 mentions) Dithiothreitol (dtt), by GoldBio (1 mentions) Tris hcl, by Avantor (1 mentions) Tris 2 carboxyethyl phosphine tcep, by GoldBio (1 mentions) Guanidine hcl, by Avantor (1 mentions) Pet his6 sumo tev lic cloning vector, by Addgene (1 mentions) Sephadex g 25 resin, by Cytiva (1 mentions) Gentamicin, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions)

5 protocols using glutamine gln

1

Cell Culture Maintenance Protocol

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Vero cells (ATCC number CCL-81) were maintained at 37°C/5 % CO2 in minimal essential medium (Life Technologies) with 7 % heat-inactivated foetal bovine serum (FBS; Life Technologies), 1 % penicillin/streptomycin (PS; 5000 U.ml−1 and 5000 μg.ml−1; Life Technologies) and 1% Glutamine (Gln; 200 mmol.l−1; Life Technologies). HEK-293 cells (ATCC number CCL-1573) were maintained at 37°C/ 5 % CO2 in the same medium supplemented with 1 % non-essential amino acids (Life Technologies).
Mohamed Ali S., Vega-Rúa A., Driouich J.S., de Lamballerie X., Failloux A.B, & Nougairède A. (2018). Comparison of chikungunya viruses generated using infectious clone or the Infectious Subgenomic Amplicons (ISA) method in Aedes mosquitoes. PLoS ONE, 13(6), e0199494.
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2

Culturing Vero and HEK-293 Cells

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Vero ATCC cells (derived from the kidney of an African green monkey; ATCC number CCL-81) were cultured at 37 °C with 5 % CO2 in minimal essential medium (Life Technologies) with 7 % heat-inactivated fetal bovine serum (FBS; Life Technologies), 1 % penicillin/streptomycin (PS; 5000 U ml−1 and 5000 µg ml−1; Life Technologies) and 1% glutamine (Gln; 200 mmol l−1; Life Technologies). HEK-293 cells (ATCC number CCL-1573) were cultured at 37 °C with 5 % CO2 in the same medium used for the Vero ATCC cells supplemented with 1 % non-essential amino acids (Life Technologies).
Driouich J.S., Ali S.M., Amroun A., Aubry F., de Lamballerie X, & Nougairède A. (2018). SuPReMe: a rapid reverse genetics method to generate clonal populations of recombinant RNA viruses. Emerging Microbes & Infections, 7, 40.
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3

Synthesis and Purification of Proteins

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Co(Cl)2·6H2O, CH3I, and iPrBr were obtained from Sigma-Aldrich; Co(OAc)2·3H2O was obtained from Mallinckrodt; 4-methylpyridine (pyMe) and 4-vinylpyridine (pyv) were obtained from Oakwood Chemicals; and all deuterated solvents were purchased from Cambridge Isotope Laboratory. Dry solvents were obtained from a Jorg. C. Meyer solvent purification system. Ni-NTA resin was obtained from Cube BioTech and Thermo Fisher Scientific, while Sephadex G25 resin was obtained from Cytiva. Guanidine HCl and Tris HCl were purchased from VWR. Dithiothreitol (DTT) and tris(2-carboxyethyl)phosphine (TCEP) were purchased from Gold-Bio. Isopropylthio-β-D-galactoside (IPTG), 5,5-dithio-bis(2-nitrobenzoic acid) (DTNB), and glutamine (Gln) were purchased from Thermo Fisher Scientific. All chemicals were used as ordered without further purification. All buffers were prepared using 18.2 MΩ cm resistivity water from a Milli-Q water purification system. The pET His6 SUMO TEV LIC cloning vector was purchased from Addgene, and E. coli BL21 (DE3) cells were purchased from Novagen. TEV protease was expressed and purified according to literature protocols.
Neuwirth C., Madec S., Siebor E., Pechinot A., Duez J.M., Pruneaux M., Fouchereau-Peron M., Kazmierczak A, & Labia R. (2001). TEM-89 β-Lactamase Produced by a Proteus mirabilis Clinical Isolate: New Complex Mutant (CMT 3) with Mutations in both TEM-59 (IRT-17) and TEM-3. Antimicrobial Agents and Chemotherapy, 45(12), 3591-3594.
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4

Isolation of Human Whole Blood Leukocytes

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Blood was collected from healthy human volunteers (3 volunteers, Etablissement Français du Sang, EFS, Clermont-Ferrand, France). Donors gave their written informed consent for the use of blood samples for research purposes under EFS contract no. 16-21-62 (in accordance with articles L1222-1, L1222-8, L1243-4 and R1243-61 of the French Public Health Code). Whole blood leukocytes were obtained via hemolytic shock using ammonium chloride solution (NH4Cl 115 µM; NaHCO3 12 µM, EDTA 0.01 µM), followed by centrifugation (1300 rpm, 10 min), and they were washed and suspended in Roswell Park Memorial Institute 1640 Medium (RPMI-1640, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and supplemented with fetal bovine serum (FBS, 10%) (Eurobio Scientific, Saclay, France), gentamicin (50 μg/mL), and glutamine (Gln, 2 mM) (Thermo Fisher Scientific).
Chervet A., Nehme R., Decombat C., Longechamp L., Habanjar O., Rousset A., Fraisse D., Blavignac C., Filaire E., Berthon J.Y., Delort L, & Caldefie-Chezet F. (2023). Exploring the Therapeutic Potential of Ampelopsis grossedentata Leaf Extract as an Anti-Inflammatory and Antioxidant Agent in Human Immune Cells. International Journal of Molecular Sciences, 25(1), 416.
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5

Culture and Maintenance of Vero and HEK-293 Cells

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Vero cells (derived from an African green monkey kidney; ATCC number CCL-81) were grown at 37 °C with 5% CO2 in a minimal essential medium (Thermo Scientific, Waltham, MA, USA) with 7% heat-inactivated fetal bovine serum (FBS; Thermo Scientific), 1% penicillin/streptomycin (PS; 5000 U mL−1 and 5000 µg mL−1; Thermo Scientific), and 1% glutamine (Gln; 200 mmol L−1; Thermo Scientific). HEK-293 cells were grown at 37 °C with 5% CO2 in the same medium as the Vero cells, supplemented with 1% non-essential amino acids (Thermo Scientific).
Three week old C57Bl/6J female mice were provided by Charles River laboratories.
Driouich J.S., Moureau G., de Lamballerie X, & Nougairède A. (2019). Reverse Genetics of RNA Viruses: ISA-Based Approach to Control Viral Population Diversity without Modifying Virus Phenotype. Viruses, 11(7), 666.
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