Hematoxylin h8070

The bile duct tissues of each group were immersed in 10% neutral formaldehyde solution, and paraffin sections were prepared. The specimens were fixed with 10% neutral formaldehyde solution, dehydrated in alcohol, cleared twice with xylene, immersed in wax, embedded in paraffin and cut into 4-µm sections. Thereafter, the paraffin sections were sliced continuously and placed in an oven at 80°C for 1 h. Following cooling, the sections were dehydrated in conventional gradient alcohol, cleared with xylene and then washed with PBS. The sections were stained with hematoxylin (H8070; 5 g; Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) for 3 min, removed and washed. The sections were then differentiated with hydrochloric acid alcohol for 10 sec, washed and soaked for 5 min, and reconverted to blue with ammonia for 10 min, followed by staining with eosin solution (cat. no. PT001; Shanghai Bogoo Biological Technology Co., Ltd., Shanghai, China) for a few seconds. Thereafter, the sections were dehydrated with gradient alcohol for 1 min/time and cleared twice with xylene (1 min/time). In the ventilator, following mounting of the sections with neutral gum, pathological changes were observed under an optical microscope (DMM-300D; Shanghai Caikon Optical Instrument Co., Ltd., Shanghai, China).