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2 protocols using 4 chlorodiazepam

1

Pharmacological Agents Protocol

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The following drugs were purchased from Sigma-Aldrich Chemie GmbH, Germany: isoproterenol hydrochloride, 4′-Chlorodiazepam, L-NAME hydrochloride (Nω-Nitro-l-arginine methyl ester hydrochloride). Acepromazine (NeurotranqR) was obtained from Alfasan International B.V., JA Woerden, The Netherlands, and ketamine hydrochloride (Ketamidor 10%R) from Richter Pharma AG, Wels, Austria.
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2

Quantitative Analysis of Benzodiazepines

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4'-chlorodiazepam, alprazolam, clonazepam, desalkylflurazepam, diazepam, flunitrazepam, nitrazepam, oxazepam, prazepam and temazepam were obtained from Sigma-Aldrich (Dorset, UK). 3-hydroxyphenazepam, deschloroetizolam, diclazepam, etizolam, flubromazepam, flubromazolam, meclonazepam, phenazepam and pyrazolam were obtained from Chiron (Trondheim, Norway). All compounds were received as powdered solids.
Dimethyl sulfoxide (DMSO), methanol, phosphoric acid, sodium hydrogen phosphate heptahydrate, sodium dihydrogen phosphate, disodium hydrogen phosphate, acetic acid, sodium acetate trihydrate, boric acid, sodium hydroxide, hydrochloric acid, sodium chloride and octan-1-ol were purchased from Fisher Scientific (Leicestershire, UK). Phosphate buffered saline (PBS) tablets were purchased from Sigma-Aldrich (Dorset, UK).
Human plasma (pooled, from three male donors and three female donors) was obtained from Seralab (West Sussex, UK). Plasma was received frozen with sodium citrate as an anticoagulant.
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